期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 11, 页码 2514-2517出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00295
关键词
encapsulin; nanocompartments; protein engineering
资金
- Emory University Research Committee
- U.S. National Science Foundation [CBET-1706891]
Encapsulins are robust and engineerable proteins that form hollow, nanosized, icosahedral capsids, making them attractive vehicles for drug delivery, scaffolds for synthetic bionanoreactors, and artificial organelles. A major limitation of native encapsulins is the small size of pores in the protein shell. At 3 angstrom diameter, these pores impose significant restrictions on the molecular weight and diffusion rate of potential substrates. By redesigning the pore-forming loop region in encapsulin from Thermotoga maritima, we successfully enlarged pore diameter up to an estimated 11 angstrom and increased mass transport rates by 7-fold as measured by lanthanide ion diffusion assay. Our study demonstrates the high tolerance of encapsulin for protein engineering and has created a set of novel, functionally improved scaffolds for applications as bionanoreactors.
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