期刊
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
卷 1853, 期 3, 页码 671-684出版社
ELSEVIER
DOI: 10.1016/j.bbamcr.2014.12.032
关键词
Kinetochore; Meiosis; Budding yeast; Ctf3; Ctf19; Spindle pole body
资金
- DST [SR/SO/BB-57/2009]
- DBT [BT/PR13962/BRB/10/798/2010]
- CSIR [38(1267)/10/EMR-II, EU-IV/2008/JUNE/327214, 20-6/2009(i)EU-IV/329667]
- SEED Grant from IITB of Govt. of India [09IRCC002]
During mitosis and meiosis, kinetochore, a conserved multi-protein complex, connects microtubule with the centromere and promotes segregation of the chromosomes. In budding yeast, central kinetochore complex named Ctf19 has been implicated in various functions and is believed to be made up of three biochemically distinct subcomplexes: COMA, Ctf3 and Iml3-Chl4. In this study, we aimed to identify whether Ctf3 and COMA subcomplexes have any unshared function at the kinetochore. Our data suggests that both these subcomplexes may work as a single functional unit without any unique functions, which we tested. Analysis of severity of the defects in the mutants suggests that COMA is epistatic to Ctf3 subcomplex. Interestingly, we noticed that these subcomplexes affect the organization of mitotic and meiotic kinetochores with subtle differences and they promote maintenance of Cse4 at the centromeres specifically during meiosis which is similar to the role of Mis6 (Ctf3 homolog) in fission yeast during mitosis. Interestingly, analysis of cY3 Delta and ctf19 Delta, mutants revealed a novel role of Ctf19 complex in regulation of SPB cohesion and duplication in meiosis. (C) 2015 Elsevier B.V. All rights reserved.
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