Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Biochemistry & Molecular Biology
Kuo-An Chu, Chang-Ching Yeh, Chun-Hsiang Hsu, Chien-Wei Hsu, Fu-Hsien Kuo, Pei-Jiun Tsai, Yu-Show Fu
Summary: This study compared the effectiveness of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) versus adipose tissue-derived mesenchymal stem cells (ADMSCs) for reversing pulmonary fibrosis in rats. The results showed that HUMSCs had better effects on lung function improvement and collagen degradation, as well as alveolar regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Hana Drobiova, Sardar Sindhu, Rasheed Ahmad, Dania Haddad, Fahd Al-Mulla, Ashraf Al Madhoun
Summary: Accumulating evidence suggests that the therapeutic potential of primary Wharton's jelly mesenchymal stem cells (WJ-MSCs) mainly lies in their ability to modulate the microenvironment by releasing bioactive molecules and factors, collectively known as secretome. These bioactive molecules and factors can be released directly into the microenvironment or be carried by extracellular vesicles such as exosomes and microvesicles. The secretome of WJ-MSCs contains various types of bioactive molecules and factors that have immunomodulatory, tissue replenishment, cellular homeostasis, anti-inflammatory, and anti-fibrotic effects. This review highlights the current research advances and potential clinical applications of the WJ-MSCs' secretome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Veterinary Sciences
Min-Soo Seo, Kyung-Ku Kang, Se-Kyung Oh, Soo-Eun Sung, Kil-Soo Kim, Young-Sam Kwon, Sungho Yun
Summary: Wharton's jelly is a known source of mesenchymal stem cells in various species, but there have been no reports on cats. This study isolated and characterized mesenchymal stem cells from the Wharton's jelly of cats, demonstrating their differentiation potential and therapeutic applications in feline medicine.
VETERINARY SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hong Thi Nguyen, Kasem Theerakittayakorn, Sirilak Somredngan, Apichart Ngernsoungnern, Piyada Ngernsoungnern, Pishyaporn Sritangos, Mariena Ketudat-Cairns, Sumeth Imsoonthornruksa, Juthaporn Assawachananont, Nattawut Keeratibharat, Rangsirat Wongsan, Ruttachuk Rungsiwiwut, Chuti Laowtammathron, Nguyen Xuan Bui, Rangsun Parnpai
Summary: This study reveals that WJ-MSCs have the potential to differentiate into CECs, which would be beneficial for further applications in LSCD treatment therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Stefanska, Lucie Nemcova, Malgorzata Blatkiewicz, Wojciech Pienkowski, Marcin Rucinski, Maciej Zabel, Paul Mozdziak, Marzenna Podhorska-Okolow, Piotr Dziegiel, Bartosz Kempisty
Summary: Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) possess multilineage differentiation potential and specific surface markers. In this study, RNA sequencing analysis revealed differentially expressed genes associated with apoptosis and identified upregulation of ZBTB16 and FOXO1, as well as downregulation of TGFA, in all differentiated groups. Additionally, several novel marker genes related to WJ-MSCs differentiation were identified. This study sheds light on the molecular mechanisms involved in long-term culture and differentiation of WJ-MSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Marta Dymowska, Aleksandra Aksamit, Katarzyna Zielniok, Monika Kniotek, Beata Kaleta, Aleksander Roszczyk, Michal Zych, Filip Dabrowski, Leszek Paczek, Anna Burdzinska
Summary: Although both BM-MSCs and WJ-MSCs have the ability to interact reciprocally with macrophages, the source of MSCs could slightly but significantly modify the response under clinical settings. WJ-MSCs secreted significantly more IL-6 than BM-MSCs and showed higher responsiveness to M1-derived chemotactic signals.
Article
Cell & Tissue Engineering
Beilei Ma, Tengkai Wang, Juan Li, Qian Wang
Summary: This study found that ECM derived from Wharton's Jelly-derived mesenchymal stem cells could improve the angiogenesis ability of HUVECs in vitro in a time-dependent manner. Mechanically, WJ-MSCs ECM activated the FAK/P38 signaling pathway via integrin alpha V beta 3 to promote c-Myc expression, subsequently increasing VEGF expression through P300-mediated histone acetylation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Karolina Salwierak-Glosna, Pawel Piatek, Malgorzata Domowicz, Mariola Swiderek-Matysiak
Summary: Multiple sclerosis (MS) is an autoimmune neurological disorder. Experimental therapies using mesenchymal stem cells (MSCs) have shown promising immunomodulatory potential. In this study, we investigated the effect of cerebrospinal fluid (CSF) obtained from MS patients on the secretory activity of Wharton's jelly-derived MSCs (WJ-MSCs), and further explored the interactions between WJ-MSCs and human oligodendroglia cell line (OLs). Our results demonstrate the diverse immunomodulatory properties of WJ-MSCs and how these effects can be influenced by the transplantation milieu.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maria Jose Gonzalez-Riquelme, Fernando Lucas-Ruiz, Caridad Galindo-Romero, Raquel Boia, Antonio Francisco Ambrosio, Manuel Vidal-Sanz, Ana Raquel Santiago, Marta Agudo-Barriuso
Summary: This study compares retinal organotypic cultures (ROCs) with in vivo models to study retinal ganglion cell (RGC) death and glial activation. The results show that there are significant differences in glial activation between ROCs and in vivo models, with earlier and different morphological activation in ROCs. Additionally, microglial cell density in ROCs is consistently lower than in vivo. ROCs can be used for proof-of-concept studies on neuroprotection, but long-term experiments should be performed in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Penghong Chen, Shijie Tang, Ming Li, Dezhi Wang, Caixiang Chen, Yiqun Qiu, Zhuoqun Fang, Haoruo Zhang, Hangqi Gao, Haiyan Weng, Kailun Hu, Jian Lin, Qingxia Lin, Yi Tan, Shirong Li, Jinghua Chen, Liangwan Chen, Xiaosong Chen
Summary: This study systematically analyzed the characteristics of Wharton's jelly mesenchymal stem cells (WJ-MSCs) using single-cell and spatial transcriptome sequencing. Four subpopulations of WJ-MSCs were identified, and their transcriptomes, cellular heterogeneity, and cell-state trajectories were characterized. The biofunctional-type MSCs showed promising wound repair properties. Additionally, it was found that a specific subpopulation derived from the fetal segment of the umbilical cord might have potential as an ideal therapeutic agent for wound healing.
Article
Polymer Science
Perng Yang Puah, Pak Yan Moh, Coswald Stephen Sipaut, Ping Chin Lee, Siew Eng How
Summary: Peptide-conjugated multilayer graphene oxide film was successfully fabricated and used for culturing human Wharton's jelly-derived mesenchymal stem cells. It was found that the peptide conjugate on the film supported the growth of WJ-MSCs and enhanced their osteogenic differentiation compared to the parent m-GO film, indicating its potential in bone tissue regeneration.
Article
Biochemistry & Molecular Biology
Monika Sypecka, Aleksandra Bzinkowska, Dorota Sulejczak, Filip Dabrowski, Anna Sarnowska
Summary: Wharton's jelly (WJ) from the umbilical cord (UC) is a good source of mesenchymal stem/stromal cells (MSCs) for therapy. Protocols for UC transportation, cell isolation, cultivation, and banking should be unified and optimized. WJ-MSCs showed higher levels of cytokines and chemokines secretion compared to WJ bioptats, and exhibited neural markers when exposed to cerebrospinal fluid (CSF). A protocol for manufacturing an advanced-therapy medicinal product was developed based on the findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Armando Lopez-Guillermo, Miguel Angel Canales, Ivan Dlouhy, Santiago Mercadal, Javier Briones, Alejandro Martin Garcia-Sancho, Juan Manuel Sancho, Jose Maria Moraleda, Maria Jose Terol, Antonio Salar, Luis Palomera, Santiago Gardella, Isidro Jarque, Secundino Ferrer, Joan Bargay, Andres Lopez, Carlos Panizo, Anna Muntanola, Carlos Montalban, Eulogio Conde, Miguel T. Hernandez, Alfons Soler, Jose A. Garcia Marco, Guillermo Deben, Julian Marin, Jose Francisco Tomas
Summary: In this study, no significant differences were found between consolidation and maintenance in FL patients responding to R-CHOP, although consolidation therapy was associated with higher late toxicity, requiring closer monitoring.
LEUKEMIA & LYMPHOMA
(2022)
Article
Critical Care Medicine
Sara Fernandez, Ana B. Moreno-Castano, Marta Palomo, Julia Martinez-Sanchez, Sergi Torramade-Moix, Adrian Tellez, Helena Ventosa, Ferran Segui, Gines Escolar, Enric Carreras, Josep M. Nicolas, Edward Richardson, David Garcia-Bernal, Carmelo Carlo-Stella, Jose M. Moraleda, Paul G. Richardson, Maribel Diaz-Ricart, Pedro Castro
Summary: COVID-19 patients exhibit increased circulating biomarkers of endothelial damage, complement activation, and fibrinolytic dysregulation, associated with disease severity. COVID-19 endotheliopathy differs from sepsis syndrome, in which endothelial damage is also a critical feature of pathobiology.
Article
Biochemistry & Molecular Biology
Marina Martinez-Carmona, Fernando Lucas-Ruiz, Alejandro Gallego-Ortega, Caridad Galindo-Romero, Maria Norte-Munoz, Maria Jose Gonzalez-Riquelme, Francisco J. Valiente-Soriano, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: Ly6c can serve as a specific marker of mouse vasculature, particularly of the retinal vascular plexuses. In injured retinas, Ly6c expression increases, correlating with vascular formation and classical monocyte/macrophage activation. This study provides a new tool for qualitative and quantitative assessment of vascular changes in health and disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Maria Luisa Molina, David Garcia-Bernal, Maria Dolores Salinas, Gonzalo Rubio, Pedro Aparicio, Jose M. Moraleda, Salvador Martinez, Rut Valdor
Summary: The study demonstrates the crucial role of chaperone-mediated autophagy (CMA) in the progression of glioblastoma (GB). The ablation of CMA in pericytes (PC) is identified as a key target mechanism for successful GB treatment. Additionally, new target markers related to tumor immune responses are identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Medicine, General & Internal
Maria Sanchez-Villalobos, Miguel Blanquer, Jose M. Moraleda, Eduardo J. Salido, Ana B. Perez-Oliva
Summary: In this article, the main pathophysiological pathways involved in beta-thalassemia are reviewed, with a focus on the development of new therapies directed at new therapeutic targets.
FRONTIERS IN MEDICINE
(2022)
Review
Anatomy & Morphology
Caridad Galindo-Romero, Maria Norte-Munoz, Alejandro Gallego-Ortega, Kristy T. Rodriguez-Ramirez, Fernando Lucas-Ruiz, Maria Josefa Gonzalez-Riquelme, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: This article summarizes the main topographical and functional characteristics of the rat retina, focusing on retinal ganglion cells and photoreceptors. Understanding the normal state of these neurons is crucial for studying neuronal survival and protection.
FRONTIERS IN NEUROANATOMY
(2022)
Meeting Abstract
Ophthalmology
Francisco M. Nadal-Nicolas
ACTA OPHTHALMOLOGICA
(2022)
Meeting Abstract
Ophthalmology
Manuel Vidal-Sanz, B. Vidal-Villegas, A. Gallego-Ortega, G. Rovere, F. M. Nadal-Nicolas, J. Di Pierdomenico, M. Aviles-Trigueros, M. Agudo-Barriuso, M. P. Villegas-Perez
ACTA OPHTHALMOLOGICA
(2022)
Article
Pharmacology & Pharmacy
Jose Luis Sanz, Sergio Lopez-Garcia, Leopoldo Forner, Francisco Javier Rodriguez-Lozano, David Garcia-Bernal, Sonia Sanchez-Bautista, Clara Puig-Herreros, Vicent Rosell-Clari, Ricardo E. Onate-Sanchez
Summary: This study assessed the influence of eucalyptol, chloroform, and Endosolv on the proliferative capability, cell viability, and migration rates of human periodontal ligament stem cells in vitro. The results showed that chloroform and eucalyptol exhibited higher cytotoxicity on the cells compared to the control group, while Endosolv showed similar cytotoxicity levels. The cytotoxicity of the tested solvents increased in a dose-dependent manner.
Article
Biochemistry & Molecular Biology
Maria Jose Gonzalez-Riquelme, Fernando Lucas-Ruiz, Caridad Galindo-Romero, Raquel Boia, Antonio Francisco Ambrosio, Manuel Vidal-Sanz, Ana Raquel Santiago, Marta Agudo-Barriuso
Summary: This study compares retinal organotypic cultures (ROCs) with in vivo models to study retinal ganglion cell (RGC) death and glial activation. The results show that there are significant differences in glial activation between ROCs and in vivo models, with earlier and different morphological activation in ROCs. Additionally, microglial cell density in ROCs is consistently lower than in vivo. ROCs can be used for proof-of-concept studies on neuroprotection, but long-term experiments should be performed in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Jose Maria Cabrera-Maqueda, Raquel Boia, Fernando Lucas-Ruiz, Maria Jose Gonzalez-Riquelme, Antonio Francisco Ambrosio, Ana Raquel Santiago, Manuel Vidal-Sanz, Marta Agudo-Barriuso, Caridad Galindo-Romero
Summary: The main purpose of this study is to analyze the effects of unilateral optic nerve crush on the gene expression of pro- and anti-inflammatory mediators, and gliosis markers in injured and contralateral retinas. The results showed that both sham surgery and optic nerve crush changed the profile of inflammatory and gliosis markers in the injured and contralateral retinas, with more pronounced changes in optic nerve crush.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Oncology
Eduardo Ramos Elbal, Jose Luis Fuster, Jose Antonio Campillo, Ana Maria Galera, Mar Bermudez Cortes, Maria Esther Llinares, Irene Jimenez, Mercedes Plaza, Helios Martinez Banaclocha, Jose Antonio Galian, Miguel Blanquer Blanquer, Maria Victoria Martinez Sanchez, Manuel Muro, Alfredo Minguela
Summary: In this study, three MRD monitoring methods (MFC, FISH, and PCR) were used to anticipate relapse of childhood AML. However, preemptive therapies were not effective in preventing disease progression. Therefore, more sensitive MRD monitoring methods and more effective preemptive therapies are needed.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2023)
Article
Zoology
Francisco M. Nadal-Nicolas, Caridad Galindo-Romero, Fernando Lucas-Ruiz, Nicholas Marsh-Amstrong, Wei Li, Manuel Vidal-Sanz, Marta Agudo-Barriuso
Summary: Univocal identification of retinal ganglion cells (RGCs) is necessary for studying their degeneration and neuroprotection. Retrograde tracing of retinorecipient areas is an invasive technique used in the past, but not feasible in higher mammals. This review analyzed nine markers used to identify RGCs in rats, mice, and macaques, and found BRN3A and RBPMS to be the best markers in terms of specificity and labeling proportion.
ZOOLOGICAL RESEARCH
(2023)
Meeting Abstract
Hematology
Francisco Jose Lopez-Jaime, Olga Benitez, Nuria Caballero, Ruben Berrueco, Eva Alvarez, Ihosvany Fernandez-Bello, Miguel Blanquer Blanquer, Adrian Montano