Article
Biochemistry & Molecular Biology
Steffen Dickopf, Can Buldun, Vedran Vasic, Guy Georges, Carina Hage, Klaus Mayer, Matthias Forster, Uwe Wessels, Kay-Gunnar Stubenrauch, Jorg Benz, Andreas Ehler, Matthias E. Lauer, Philippe Ringler, Sebastian Kobold, Stefan Endres, Christian Klein, Ulrich Brinkmann
Summary: The field of multi-specific antibody derivatives is rapidly growing, with domain exchange reactions being able to generate hybrid antibodies under physiological conditions for potential therapeutic applications.
BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Chi Ho Wai, Jessica Jin, Marek Cyrklaff, Christel Genoud, Charlotta Funaya, Julia Sattler, Aleksandra Maceski, Stephanie Meier, Sabine Heiland, Michael Lanzer, Friedrich Frischknecht, Jens Kuhle, Martin Bendszus, Angelika Hoffmann
Summary: This study assessed whether serum levels of neurofilament light chain (NfL) can indicate the severity of cerebral malaria. The results showed that although NfL plasma levels were elevated during the disease, they were not able to reliably predict the final outcomes.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Craig Fenwick, Priscilla Turelli, Dongchun Ni, Laurent Perez, Kelvin Lau, Cecile Herate, Romain Marlin, Erica Lana, Celine Pellaton, Charlene Raclot, Line Esteves-Leuenberger, Jeremy Campos, Alex Farina, Flurin Fiscalini, Nathalie Dereuddre-Bosquet, Francis Relouzat, Rana Abdelnabi, Caroline S. Foo, Johan Neyts, Pieter Leyssen, Yves Levy, Florence Pojer, Henning Stahlberg, Roger LeGrand, Didier Trono, Giuseppe Pantaleo
Summary: A monoclonal antibody named P2G3 isolated from a previously infected vaccinated donor shows strong neutralizing activity against the SARS-CoV-2 Omicron variant and provides complete prophylactic or therapeutic protection when used alone or in combination with another antibody. The unique epitope of P2G3 may have important implications for combating the Omicron variant.
NATURE MICROBIOLOGY
(2022)
Article
Clinical Neurology
Nour Majbour, Jan Aasly, Ilham Abdi, Simona Ghanem, Daniel Erskine, Wilma van de Berg, Omar El-Agnaf
Summary: This study demonstrated the quantification of aSyn aggregates in PD patients through a combination of seed amplification assay and ELISA, showing that this method is more effective than using SAA alone and can reflect the disease stage of patients.
Article
Biochemistry & Molecular Biology
Jaroslaw Sedzicki, Dongchun Ni, Frank Lehmann, Na Wu, Renato Zenobi, Seunho Jung, Henning Stahlberg, Christoph Dehio
Summary: Polysaccharides play critical roles in bacteria and their transport is mediated by ATP-binding cassette (ABC) transporters. This study used cryoelectron microscopy to elucidate the structures of Cgt from Brucella abortus and revealed the binding mode and substrate release mechanism, providing insights into the translocation of large substrates and protein-polysaccharide interactions.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Babatunde Ekundayo, Davide Torre, Bertrand Beckert, Sergey Nazarov, Alexander Myasnikov, Henning Stahlberg, Dongchun Ni
Summary: The CRISPR-guided caspase (Craspase) complex consists of Cas7-11, CRISPR RNA (crRNA), and TPR-CHAT protein. It shows potential as a tool for gene therapy and biomedical research, but its regulation is not well understood. In this study, cryoelectron microscopy was used to determine the structures of the Dm Craspase complex to understand its regulation mechanism. It was found that DmTPR-CHAT stabilizes crRNA-bound DmCas7-11 in a closed conformation through interactions mediated by DmTPR-CHAT N-terminal domain, DmCas7-11 insertion finger, and Cas11-like domain, resulting in reduced target RNA accessibility and cleavage.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Correction
Biology
Valerie Biou, Ricardo Jorge Diogo Adaixo, Mohamed Chami, Pierre-Damien Coureux, Benoist Laurent, Veronique Yvette Ntsogo Enguene, Gisele Cardoso de Amorim, Nadia Izadi-Pruneyre, Christian Malosse, Julia Chamot-Rooke, Henning Stahlberg, Philippe Delepelaire
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Pablo Gainza, Sarah Wehrle, Alexandra Van Hall-Beauvais, Anthony Marchand, Andreas Scheck, Zander Harteveld, Stephen Buckley, Dongchun Ni, Shuguang Tan, Freyr Sverrisson, Casper Goverde, Priscilla Turelli, Charlene Raclot, Alexandra Teslenko, Martin Pacesa, Stephane Rosset, Sandrine Georgeon, Jane Marsden, Aaron Petruzzella, Kefang Liu, Zepeng Xu, Yan Chai, Pu Han, George F. Gao, Elisa Oricchio, Beat Fierz, Didier Trono, Henning Stahlberg, Michael Bronstein, Bruno E. Correia
Summary: Physical interactions between proteins are crucial for biological processes. A geometric deep-learning framework is used to generate fingerprints on protein surfaces to describe the key aspects of molecular recognition. Using this approach, several de novo protein binders were designed and successfully bound to different protein targets.
Article
Cell Biology
Carmela Conte, Angela Ingrassia, John Breve, John J. J. Bol, Evelien Timmermans-Huisman, Anne-Marie van Dam, Tommaso Beccari, Wilma D. J. van de Berg
Summary: This study found an increased expression of Toll-like receptor 4 (TLR4) in the brains of Parkinson's disease (PD) patients, which colocalized with phosphorylated α-synuclein (pSer129-aSyn) at the site of damage, suggesting a potential role in mediating the neuroinflammatory response in PD.
Article
Neurosciences
Elvira S. Amaral Gomes, Odile A. van den Heuvel, Marc B. Rietberg, Vincent De Groot, Mark A. Hirsch, Wilma D. J. van de Berg, Richard T. Jaspers, Chris Vriend, Tim Vanbellingen, Erwin E. H. Van Wegen
Summary: This pilot study aimed to quantify individual response patterns of motor symptoms, non-motor functioning, and blood biomarkers in Parkinson's disease patients to different exercise modalities. The results showed that engaging in physical exercise can reduce motor symptoms, but there is significant variability in individual response patterns among participants.
Article
Biochemistry & Molecular Biology
Dagmar H. Hepp, Thecla A. van Wageningen, Kirsten L. Kuiper, Karin D. van Dijk, Linda P. Oosterveld, Henk W. Berendse, Wilma D. J. van de Berg
Summary: An altered immune response has been found in Parkinson's disease, with specific blood proteins associated with disease severity. Over time, the expression levels of inflammation-associated proteins may increase and are correlated with the severity of motor and cognitive symptoms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Galina Limorenko, Meltem Tatli, Rajasekhar Kolla, Sergey Nazarov, Marie-Theres Weil, David C. Schondorf, Daniela Geist, Peter Reinhardt, Dagmar E. Ehrnhoefer, Henning Stahlberg, Laura Gasparini, Hilal A. Lashuel
Summary: The authors present a method to produce co-factor-free fibrils from all full-length Tau isoforms, which allows for the reconstitution of pathology resembling Tau fibrils and the screening of Tau aggregation-modifying compounds for targeted therapies and PET tracers.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Dongchun Ni, Xuhang Lu, Henning Stahlberg, Babatunde Ekundayo
Summary: Short prokaryotic argonaute (pAgo) and toll/interleukin-1 receptor/resistance protein (TIR)-analog of PAZ (APAZ) form a heterodimeric SPARTA complex that provides immunity to its prokaryotic host through an abortive infection mechanism. Monomeric SPARTA senses foreign RNA/DNA duplexes to assemble an active tetramer resulting in cell death by NAD depletion via an unknown mechanism. Our findings provide detailed structural and mechanistic insights into activating a short argonaute defense system.
Meeting Abstract
Biochemistry & Molecular Biology
Alexandra Van Hall-Beauvais, Pablo Gainza, Sarah Wehrle, Andreas Scheck, Anthony Marchand, Zander Harteveld, Casper Goverde, Dongchun Ni, Tan Shuguang, Priscilla Turelli, Didier Trono, Henning Stahlberg, Geroge Gao, Michael Bronstein, Bruno Correia
Article
Chemistry, Physical
Luca Rima, Michael Zimmermann, Andri Fraenkl, Thomas Clairfeuille, Matthias Lauer, Andreas Engel, Hans-Andreas Engel, Thomas Braun
Summary: This article presents an amended microfluidic sample preparation method that significantly improves the quality of the sample by avoiding paper blotting and protecting the air-water interface.
FARADAY DISCUSSIONS
(2022)