Article
Biochemical Research Methods
Shuang Fu, Wei Shi, Tingdan Luo, Yingchuan He, Lulu Zhou, Jie Yang, Zhichao Yang, Jiadong Liu, Xiaotian Liu, Zhiyong Guo, Chengyu Yang, Chao Liu, Zhen-li Huang, Jonas Ries, Mingjie Zhang, Peng Xi, Dayong Jin, Yiming Li
Summary: We propose a deep-learning method for precise localization of spatially variant point emitters, covering the full chip of a modern sCMOS camera. By using a graphic processing unit-based vectorial point spread function fitter, we accurately model the spatially variant PSF of a high numerical aperture objective in the entire field of view. Combined with deformable mirror-based optimal PSF engineering, we demonstrate high-accuracy three-dimensional single-molecule localization microscopy over a large volume, enabling imaging of mitochondria and nuclear pore complexes in entire cells without hardware scanning, with a 100-fold increase in throughput compared to existing techniques.
Article
Microbiology
Maximilian W. D. Raas, Thiago P. Silva, Jhamine C. O. Freitas, Lara M. Campos, Rodrigo L. Fabri, Rossana C. N. Melo
Summary: The study utilized whole slide imaging (WSI) to visualize Candida biofilm formation, staining accumulated biofilms with fluorescent markers and scanning in both bright-field and fluorescence modes using a WSI digital scanner. WSI allowed for clear assessment of biofilm size and structural features, with quantitative analysis showing reductions in biofilm-covered surface area upon antifungal exposure. At the single-cell level, WSI proved to be adequate for evaluating morphometric parameters, demonstrating its reliability in visualizing Candida biofilms and making it an important addition to microscopic tools for fungal biofilm growth analysis.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Analytical
Jun-Hao Wan, Chen Qian, Gang Wu, Xian-Wei Liu
Summary: Label-free imaging of nanoscale targets with intrinsic properties is crucial for unveiling the underlying mechanisms of chemistry, physics, and life science. Plasmonic imaging techniques provide real-time imaging and insights into nanoscale detection and nanocatalysis. In this study, a high-resolution plasmonic imaging method is presented, demonstrating its capability to image various nanomaterials and accurately track the interfacial dynamics of nanoparticles. With the simplicity and capacity for label-free and real-time imaging, this approach serves as a promising platform for characterizing nanomaterials at the single-particle level.
ANALYTICAL CHEMISTRY
(2023)
Article
Plant Sciences
Keith E. Duncan, Kirk J. Czymmek, Ni Jiang, August C. Thies, Christopher N. Topp
Summary: This study presents technical advances in using lab-based X-ray microscopy for high-resolution 3D imaging of plant samples at multiple scales. Serial imaging and improved sample preparation methods allow for the generation of sub-micron 3D volumes co-registered with lower magnification scans, providing explicit contextual reference. This method bridges the imaging gap between light and electron microscopy and can be applied to various economically and scientifically important plant systems.
Article
Multidisciplinary Sciences
Yuechuan Lin, Nichaluk Leartprapun, Justin C. Luo, Steven G. Adie
Summary: Quantitative characterization of micro-scale mechanical properties of the extracellular matrix (ECM) and dynamic cell-ECM interactions can significantly enhance fundamental discoveries and their translational potential in the rapidly growing field of mechanobiology. The authors introduce photonic force optical coherence elastography that uses a light-sheet for parallelised and localised mechanical loading. They demonstrate the combination of 3D imaging of extracellular matrix mechanics with cellular-scale resolution and dynamic monitoring of cell-mediated changes.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Gregory J. Hunt, Mark A. Dane, James E. Korkola, Laura M. Heiser, Johann A. Gagnon-Bartsch
Summary: In this study, we propose a general approach that leverages an experimental design with multiple levels of replication to remove technical artifacts from high-throughput image data. By applying this approach to microenvironment microarrays (MEMAs), we are able to eliminate unwanted spatial artifacts and enhance the biological signal. This method has broad applicability in various biological assays.
Article
Optics
Taeseong Woo, Hye Yun Kim, Su Yeon Kim, Byungjae Hwang, Cheolwoo Ahn, Seok-Kyu Kwon, Jae-Ick Kim, Jung-Hoon Park
Summary: Recent advances in biochemistry and optics have made it possible to observe faint signals from single molecules. However, the detection range is limited by the dynamic range of current detectors. To overcome this limitation, structured illumination high dynamic range (SI-HDR) imaging is introduced, enabling real-time HDR imaging with a single measurement. The method demonstrates wide applicability in various applications.
Article
Nanoscience & Nanotechnology
Masashi Ugawa, Sadao Ota
Summary: This study introduces a high-throughput 3D-imaging flow cytometry technique based on light-sheet microscopy, which can rapidly screen a large number of cells and obtain subcellular structural information.
Article
Chemistry, Multidisciplinary
Marti Checa, Ruben Millan-Solsona, Adrianna Glinkowska Mares, Silvia Pujals, Gabriel Gomila
Summary: Mapping the dielectric properties of cells with nanoscale spatial resolution is crucial for nanomedicine and nanotoxicity analysis, complementing structural and mechanical measurements. This study demonstrates the feasibility of obtaining dielectric constant maps of fixed cells in liquid environments, which helps preserve cell structure while accessing local dielectric properties under fully hydrated conditions. Further developments are needed to address the nanoscale dielectric imaging of living cells.
Article
Biochemical Research Methods
Tingting Zhu, Jun Nie, Tingting Yu, Dan Zhu, Yanyi Huang, Zaozao Chen, Zhongze Gu, Jiang Tang, Dongyu LI, Peng Fei
Summary: Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that improves efficiency. However, noticeable aberrations induced by the complex refractive indices in the chip limit microchip-enhanced light-sheet microscopy. This study introduces a droplet microchip with a polymer index matched to water, enabling large-scale culture of 3D spheroids and time-lapse imaging with high resolution and throughput.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Biochemical Research Methods
Lucy M. Collinson, Carles Bosch, Anwen Bullen, Jemima J. Burden, Raffaella Carzaniga, Cheng Cheng, Michele C. Darrow, Georgina Fletcher, Errin Johnson, Kedar Narayan, Christopher J. Peddie, Martyn Winn, Charles Wood, Ardan Patwardhan, Gerard J. Kleywegt, Paul Verkade
Summary: Volume electron microscopy (vEM) is a set of techniques that can reveal the 3D structure of cells and tissues at depths of at least 1 micrometer. An emerging grassroots community effort is rapidly showcasing the impact of vEM technology in life sciences and clinical research.
Article
Optics
Weisong Zhao, Shiqun Zhao, Zhenqian Han, Xiangyan Ding, Guangwei Hu, Liying Qu, Yuanyuan Huang, Xinwei Wang, Heng Mao, Yaming Jiu, Ying Hu, Jiubin Tan, Xumin Ding, Liangyi Chen, Changliang Guo, Haoyu Li
Summary: High-throughput super-resolution (SR) imaging is attractive for rapid and high-precision profiling in biomedical applications. The proposed SACD method reduces the number of frames required for SR imaging and enables four-dimensional imaging of live cells and events. SACD improves accessibility to SR imaging and facilitates high-throughput and low-cost biological studies.
Article
Multidisciplinary Sciences
Jianian Lin, Zongyue Cheng, Guang Yang, Meng Cui
Summary: The authors develop an optical gearbox for accelerating laser scanning microscopes, and validate its technology through in vivo functional imaging of mouse brains. They demonstrate high spatiotemporal resolution functional imaging in mammalian brains by using this optical gearbox. This versatile add-on module expands the capability of existing multiphoton imaging systems for versatile high-throughput imaging applications.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Electrical & Electronic
Ishtiaq Ahmad, Khoa D. Nguyen, Nick Letzepis, Gottfried Lechner, Vahid Joroughi
Summary: This paper investigates the application of zero-forcing (ZF) precoding in reducing interbeam interference in the forward link. By designing a ZF precoder with partial CSI and analyzing its performance, the study considers the impact of rain fading attenuation on the link, providing valuable insights and numerical analysis of the precoder's robustness to CSI estimation errors.
IEEE TRANSACTIONS ON VEHICULAR TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Ke Zhang, Xianglan Lu, Xuxin Chen, Roy Zhang, Kar-Ming Fung, Hong Liu, Bin Zheng, Shibo Li, Yuchen Qiu
Summary: This study initially verified the feasibility of utilizing FPM to develop a high-resolution and wide-field chromosome sample scanner.
JOURNAL OF BIOMEDICAL OPTICS
(2022)