Review
Cell Biology
Kate Dicker, Aino I. Jarvelin, Manuel Garcia-Moreno, Alfredo Castello
Summary: RNA viruses rely on cellular RNA-binding proteins to facilitate replication and spread, with a pool of RBPs typically incorporated into viral particles. These RBPs play crucial roles in viral particle formation and infectivity, suggesting broader implications for host RBPs in virus infection than previously thought.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Review
Microbiology
Jim Baggen, Els Vanstreels, Sander Jansen, Dirk Daelemans
Summary: This review summarizes proviral host factors required for SARS-CoV-2 infection, important cellular processes in SARS-CoV-2 replication, and host factors that could be targeted for antiviral therapies for COVID-19.
NATURE MICROBIOLOGY
(2021)
Article
Cell Biology
Ubaldo Gioia, Sara Tavella, Pamela Martinez-Orellana, Giada Cicio, Andrea Colliva, Marta Ceccon, Matteo Cabrini, Ana C. Henriques, Valeria Fumagalli, Alessia Paldino, Ettore Presot, Sreejith Rajasekharan, Nicola Iacomino, Federica Pisati, Valentina Matti, Sara Sepe, Matilde I. Conte, Sara Barozzi, Zeno Lavagnino, Tea Carletti, Maria Concetta Volpe, Paola Cavalcante, Matteo Iannacone, Chiara Rampazzo, Rossana Bussani, Claudio Tripodo, Serena Zacchigna, Alessandro Marcello, Fabrizio d'Adda di Fagagna
Summary: SARS-CoV-2 causes DNA damage and alters the DNA damage response, leading to inflammation and cellular senescence.
NATURE CELL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Elodie Beaumont, Vincent Larochette, Laurence Preisser, Charline Miot, Pascale Pignon, Simon Blanchard, Bjorn-Thore Hansen, Jonathan Dauve, Caroline Poli, Minna M. Poranen, Patricia Lamourette, Marc Plaisance, Alain Morel, Helmut Fickenscher, Pascale Jeannin, Philippe Roingeard, Yves Delneste
Summary: IL-26 plays a crucial role in protecting against HCV infection in the liver of patients with chronic hepatitis C, exerting direct antiviral activity by inhibiting viral replication and interfering with RNA polymerase activity.
JOURNAL OF HEPATOLOGY
(2022)
Review
Virology
Rongrong He, Yinzi Li, Mark A. Bernards, Aiming Wang
Summary: Viruses infect all cellular life forms and cause various diseases and significant economic losses worldwide. They induce altered membrane structures in infected host cells, forming viral replication organelles or complexes. These organelles provide an optimum environment for robust viral replication and can also be mobile, reaching neighboring cells via plasmodesmata through the endomembrane-cytoskeleton network.
Review
Virology
Jessica E. Packard, Jill A. Dembowski
Summary: DNA replication in HSV-1 involves a combination of viral and cellular factors, such as UL9, ICP8, UL30, UL42, and UL5/UL8/UL52 proteins, as well as accessory factors to coordinate viral DNA replication with other processes.
Article
Microbiology
Meisam Yousefi, Wai Suet Lee, Biaoguo Yan, Liang Cui, Cythia Lingli Yong, Xin Yap, Kwan Sing Leona Tay, Wenjie Qiao, Dewei Tan, Nur Insyirah Nurazmi, Martin Linster, Gavin J. D. Smith, Yie Hou Lee, Jan E. Carette, Eng Eong Ooi, Kuan Rong Chan, Yaw Shin Ooi
Summary: TMEM41B and VMP1 are ER-associated lipid scramblases that play a crucial role in autophagosome formation and cellular lipid metabolism. They are essential host dependency factors for dengue virus and human coronavirus OC43, but not chikungunya virus. TMEM41B deficiency leads to upregulation of immune response and abnormal viral replication, which can be partially reversed by exogenous fatty acid supplements.
Article
Multidisciplinary Sciences
Kang Ning, Wei Zou, Peng Xu, Fang Cheng, Elizabeth Yan Zhang, Aaron Zhang-Chen, Steve Kleiboeker, Jianming Qiu
Summary: The study identified AXL as a protein receptor for B19V to infect human erythroid progenitor cells. Knocking down the AXL gene significantly reduced B19V internalization and replication. The interaction between B19V VP1u and the extracellular domain of AXL was confirmed, suggesting the importance of AXL as a co-receptor for B19V infection.
Article
Multidisciplinary Sciences
Dong-Hwi Kim, Hee-Seop Ahn, Hyeon-Jeong Go, Da-Yoon Kim, Jae-Hyeong Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Sang-Do Ha, Changsun Choi, In-Soo Choi
Summary: HO-1 induced by hemin effectively suppresses SARS-CoV-2 replication, making it a potential therapeutic candidate for COVID-19.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yue Zhang, Shuangshuang Zhao, Yian Li, Fengping Feng, Min Li, Yanhong Xue, Jie Cui, Tao Xu, Xia Jin, Yaming Jiu
Summary: The study reveals the important role of intermediate filament protein vimentin in Zika virus infection, influencing viral replication and production. Vimentin regulates the organization of cellular structure and RNA binding, thus modulating the process of viral replication.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Enakshi Ghosh, Ghada S. Y. Tafesh-Edwards, Ioannis Eleftherianos, Stephanie L. Goldin, Paul J. Ode
Summary: Insect herbivores encountering plant defense molecules have unclear understanding of the consequences for their immune systems. Previous studies have explored the relationship between plant defensive chemistry and herbivore immune responses using natural variation, but this may confound with other plant trait differences. This study investigates the effects of a plant toxin on the immune responses of the generalist herbivore Trichoplusia ni. The results show that high concentrations of the plant toxin hindered caterpillar development, decreased cellular immunity, and had mixed effects on humoral immunity.
Article
Microbiology
Austin Royster, Songyang Ren, Yutian Ma, Melissa Pintado, Eunice Kahng, Sean Rowan, Sheema Mir, Mohammad Mir
Summary: A novel compound called K31 has been discovered to bind to the highly conserved N protein of SARS-CoV-2, inhibiting its replication. This finding suggests that targeting the viral nucleocapsid protein may be a potential approach for developing therapeutic drugs against coronaviruses.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Barbara Wrzesinska, Agnieszka Zmienko, Lam Dai Vu, Ive De Smet, Aleksandra Obrepalska-Steplowska
Summary: PSV infection results in changes in the abundance of host plant's transcripts and proteins associated with various cellular compartments, affecting processes such as photosynthesis, translation, transcription, and mRNA splicing. The study utilized a comprehensive approach to analyze small RNA, transcripts, proteins, and phosphoproteins affected during virus-plant interactions, shedding light on the cellular localization and processes implicated during pathogenesis.
PLANT CELL REPORTS
(2021)
Article
Biology
Kun Li, Ziqiang Wang
Summary: The study reveals that HSV-1 infection causes redistribution of nuclear speckles and paraspeckles components, which play a crucial role in regulating viral gene transcription by binding to specific gene promoters. This interaction influences histone modifications near viral genes, shedding light on how HSV-1 utilizes host cellular nuclear bodies to facilitate its life cycle.
COMMUNICATIONS BIOLOGY
(2021)
Article
Cell Biology
Yonghao Zhan, Aolin Li, Congcong Cao, Yuchen Liu
Summary: In this study, the CRISPR signal conductor 2.0 was developed by integrating various lncRNA functional motifs into the CRISPR-dCas phi system. This system can directly regulate target gene expression and efficiently sense protein signals undetected by classical synthetic systems.
Article
Oncology
Minhong Shen, Yi-Zhou Jiang, Yong Wei, Brian Ell, Xinlei Sheng, Mark Esposito, Jooeun Kang, Xiang Hang, Hanqiu Zheng, Michelle Rowicki, Lanjing Zhang, Weichung J. Shih, Toni Celia-Terrassa, Yirong Liu, Ileana Cristea, Zhi-Ming Shao, Yibin Kang
Article
Multidisciplinary Sciences
Yutaka Hashimoto, Xinlei Sheng, Laura A. Murray-Nerger, Ileana M. Cristea
NATURE COMMUNICATIONS
(2020)
Article
Genetics & Heredity
Michelle L. Hamm, Anarosa A. Garcia, Rachel Gilbert, Manavi Johri, Miranda Ricart, Samantha L. Sholes, Laura A. Murray-Nerger, Eugene Y. Wu
Article
Biochemistry & Molecular Biology
Laura A. Murray-Nerger, Joshua L. Justice, Pranav Rekapalli, Josiah E. Hutton, Ileana M. Cristea
Summary: Acetylation of lamin B1 at K134 is a molecular switch that controls nuclear periphery stability, cell cycle progression, and DNA repair. This modification prevents lamin disruption during HSV-1 infection, delays DNA damage resolution, and sustains activation of the G1/S checkpoint. Acetylation of LMNB1 negatively regulates canonical nonhomologous end joining and impacts DNA repair pathway choice.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Xinlei Sheng, Ileana M. Cristea
Summary: This study highlights the role of SIRT3 as a key regulator of mitochondrial acetylation and morphology during HCMV infection, affecting both the structural integrity and metabolism of mitochondria. The findings suggest that virus-induced alterations in SIRT3 functions and mitochondrial acetylation are linked to known HCMV pathologies, such as metabolic syndrome and cardiac hypertrophy. Through various analyses, the study demonstrates the importance of SIRT3 in maintaining mitochondrial health and suppressing virus production during infection.
Article
Cell Biology
Wei Shi, Xinlei Sheng, Kerry M. Dorr, Josiah E. Hutton, James Emerson, Haley A. Davies, Tia D. Andrade, Lauren K. Wasson, Todd M. Greco, Yutaka Hashimoto, Joel D. Federspiel, Zachary L. Robbe, Xuqi Chen, Arthur P. Arnold, Ileana M. Cristea, Frank L. Conlon
Summary: This study demonstrates that sex disparities in cardiac function are not only controlled by sex hormones but also through a sex-chromosome mechanism, particularly involving X-linked gene dosage. The research also shows that cardiac sex disparities occur at the earliest stages of heart formation, before gonad development. Loss of A1BG is found to lead to cardiac defects in females but not in males.
DEVELOPMENTAL CELL
(2021)
Review
Biochemistry & Molecular Biology
Laura A. Murray-Nerger, Ileana M. Cristea
Summary: Nuclear lamins are ancient type V intermediate filaments with diverse functions that are regulated by a range of post-translational modifications, playing crucial roles in maintaining nuclear shape, mechanosignaling, chromatin stability, gene expression, and cell cycle progression.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Dongdong Li, Wendan Chu, Xinlei Sheng, Wenqi Li
Summary: This study used analytical ultracentrifugation to investigate the effects of different detergents on the behavior of membrane protein TmrA. Results suggest that DDM is more suitable for the purification of TmrA, a high DDM concentration yields a more homogeneous protein aggregation state, and supplementing TmrA purified with a low DDM concentration with DDM may serve as a practical and cost-effective strategy for membrane protein purification.
Article
Cell Biology
Zachary L. Robbe, Wei Shi, Lauren K. Wasson, Angel P. Scialdone, Caralynn M. Wilczewski, Xinlei Sheng, Austin J. Hepperla, Brynn N. Akerberg, William T. Pu, Ileana M. Cristea, Ian J. Davis, Frank L. Conlon
Summary: This study investigated how CHD4/NuRD is localized to specific cardiac target genes by coupling a bioinformatics-based approach with mass spectrometry analyses. They demonstrated that CHD4 interacts with the core cardiac transcription factors GATA4, NKX2-5, and TBX5 during embryonic heart development, providing new insights into how mutations in the CHD4 protein lead to cardiac-specific disease states.
GENES & DEVELOPMENT
(2022)
Article
Cell Biology
Michelle A. Kennedy, Matthew D. Tyl, Cora N. Betsinger, Joel D. Federspiel, Xinlei Sheng, Jesse H. Arbuckle, Thomas M. Kristie, Ileana M. Cristea
Summary: This study developed a mass spectrometry-based method for detecting and quantifying viral proteins in herpesvirus infections. The method was validated and applied in various aspects, providing important insights into viral replication and potential therapeutic agents.
Editorial Material
Cell Biology
Gaoxiang Zhao, Qian Lin, Zhaoyuan Meng, Xinlei Sheng, Leina Ma, Yingming Zhao
Article
Multidisciplinary Sciences
Jinjun Gao, Xinlei Sheng, Jianfeng Du, Di Zhang, Chang Han, Yue Chen, Chu Wang, Yingming Zhao
Summary: We developed a new database search strategy called CHiMA, which uses 50% matched fragment ions as the key criterion to identify high-confidence PSMs. Compared to conventional methods, CHiMA identified twice as many histone modification sites in benchmark datasets. Reanalysis of previous proteomics data using CHiMA led to the discovery of 113 new histone marks, almost doubling the number of previously reported marks. This tool not only offers a valuable approach for identifying histone modifications but also greatly expands the repertoire of histone marks.
Article
Multidisciplinary Sciences
Bokai Song, Xinlei Sheng, Joshua L. Justice, Krystal K. Lum, Peter J. Metzger, Katelyn C. Cook, James C. Kostas, Ileana M. Cristea
Summary: Communication between infected cells and cells in the surrounding tissue is crucial for viral spread. A cell-based system was established to investigate the virus microenvironment and the responses of neighboring and distal cells to primary or secondary infections. The study reveals that human cytomegalovirus (HCMV) infection primes neighboring cells for subsequent HCMV infections and secondary infections with herpes simplex virus 1 and influenza A, while distal cells exhibit enhanced antiviral responses to slow down viral spread. These findings highlight the importance of intercellular signaling and spatial proximity in shaping the microenvironment and determining cell fate during infections.
Article
Chemistry, Multidisciplinary
Yan Gao, Xinlei Sheng, Doudou Tan, SunJoo Kim, Soyoung Choi, Sanjita Paudel, Taeho Lee, Cong Yan, Minjia Tan, Kyu Min Kim, Sam Seok Cho, Sung Hwan Ki, He Huang, Yingming Zhao, Sangkyu Lee
Summary: This study identifies acetoacetate as the precursor for lysine acetoacetylation (Kacac), a previously uncharacterized histone post-translational modification. It also finds that HBO1 can function as both an acetyltransferase and an acetoacetyltransferase. Additionally, 33 Kacac sites are identified on mammalian histones, providing insight into the distribution of this modification across species and organs.