Article
Immunology
Saeideh Sadat Shobeiri, MohammadAli Rezaee, Safoora Pordel, Navideh Haghnnavaz, Mohammadreza Dashti, Malihe Moghadam, Mojtaba Sankian
Summary: The therapeutic effects of M2 and M7 anti-IL17A ssDNA aptamers were evaluated in an IMQ-induced psoriasis mouse model. The application of aptamers significantly improved psoriatic lesions and decreased the expression levels of inflammatory factors.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Pio Conti, Fabrizio E. Pregliasco, Rosa G. Bellomo, Carla E. Gallenga, Alessandro Caraffa, Spyros K. Kritas, Dorina Lauritano, Gianpaolo Ronconi
Summary: Psoriasis is a skin disease characterized by autoimmune features and inflammatory symptoms, often accompanied by various comorbidities. Immune cells like MCs and cytokines such as IL-36 play significant roles in the inflammation process of psoriasis, while IL-38 and IL-37 may serve as potential therapeutic tools for inhibiting inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Yuan Pan, Minzhen Wang, Xiaoqing Chen, Yuxi Chen, Siming Ai, Mei Wang, Wenru Su, Dan Liang
Summary: The feedback loop of IL-23/IL-23R/PGE2/EP2+EP4/IL-23R/IL-17A plays a significant role in the pathogenesis of TAO, with IL-23R being a key target. Increased IL-38 in TAO can inhibit the expression of IL-23R and IL-17A in PBMCs and suppress inflammation in OFs. Targeting IL-23R for therapy may be effective, while IL-38 could serve as a potential therapeutic approach for TAO.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Pio Conti, Alessandro Caraffa, Carlo E. Gallenga, Rhiannon Ross, Spyros K. Kritas, Ilyas Frydas, Ali Younes, Paolo Di Emidio, Gianpaolo Ronconi, Franco Pandolfi
Summary: Luteolin is a flavonoid that can decrease reactive oxygen species levels and inhibit inflammation. IL-1 family members and Toll-like receptor play roles in the immune system, while IL-37 and IL-38 are new cytokines that suppress inflammation. Combining luteolin with these anti-inflammatory cytokines may be beneficial in treating inflammatory diseases.
Article
Immunology
Meiying Song, Jie Liang, Luoyang Wang, Wei Li, Suli Jiang, Shuo Xu, Lei Tang, Qiaochu Du, Guixian Liu, Haining Meng, Dongchang Zhai, Shangheng Shi, Yanyan Yang, Li Zhang, Bei Zhang
Summary: IL-17A, mainly produced by Th17 cells, has a host-protective capacity but excessive elevation is linked to autoimmune diseases and cancer. Monoclonal antibodies targeting IL-17A or its receptor are potential treatments for these diseases. This article reviews the mechanism of action of IL-17A and the application of anti-IL-17A antibodies in various tumors.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Dermatology
Chiara Della Bella, Alberto Corra, Elisabetta Mantengoli, Angelo Galano, Marisa Benagiano, Diletta Bonciani, Elena Biancamaria Mariotti, Sara Pratesi, Lavinia Quintarelli, Cristina Aimo, Alessia Grassi, Sofia D'Elios, Walter Volpi, Alice Verdelli, Alessandro Bartoloni, Gian Maria Rossolini, Mario Milco D'Elios, Marzia Caproni
Summary: This study aimed to investigate the correlation between IL-17A and IFN-gamma production by T cells infiltrating psoriatic skin lesions and PASI score. The results showed a significant correlation between IL-17A and IFN-gamma levels and PASI score. Among the patients, 20.4% had a streptococcal throat infection, and most of the skin-infiltrating T cells displayed a T helper 17 and T helper 1 phenotype.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Medicine, General & Internal
Georgios Kokolakis, Kamran Ghoreschi
Summary: The understanding of immunopathogenesis of psoriasis has led to the development of targeted therapies, including IL-17 antibodies which have been established for the treatment of psoriasis, psoriatic arthritis, and axial spondyloarthritis. This study presents two patients with psoriasis who lost response to previous treatments, but responded well to bimekizumab, a new antibody that neutralizes both IL-17A and IL-17F. The fast response and lack of side effects suggest the important role of IL-17F and the potential benefit of bimekizumab in non-responders to other anti-IL-17 therapies.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Olga Kostareva, Arina Svoeglazova, Ilya Kolyadenko, Alexey Nikulin, Stanislav Evdokimov, Uliana Dzhus, Azat Gabdulkhakov, Svetlana Tishchenko
Summary: Interleukin-17 (IL-17) is a cytokine involved in chronic inflammation in autoimmune diseases. Antibodies targeting IL-17 are used as therapy tools. The crystal structure of an IL-17A-specific antibody in complex with IL-17A has been determined, revealing the interaction between the antibody and specific regions of IL-17A.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Aeri Park, Tae-Hwe Heo
Summary: IL-17A is a crucial pro-inflammatory cytokine in autoimmune diseases, which can be inhibited by fenofibrate, a small molecule drug. Fenofibrate regulates autophagy and exerts anti-inflammatory effects, suggesting its potential as a therapeutic for psoriasis and other autoimmune diseases.
Article
Immunology
Qiang Dong, Dan Li, Bi Bo Xie, Li Hua Hu, Jia Huang, Xiao Xiao Jia, Yan Li Tang, Gan Hong Liu, Ning Ning Shen, Xiao Bing Yu
Summary: Treatment of psoriasis with Adalimumab and secukinumab can induce changes in multiple proteins in the lesions, overlapping effects on immune response and processes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xinran Xie, Lei Zhang, Yan Lin, Xin Liu, Ning Wang, Ping Li
Summary: In this study, a mouse model of psoriasiform dermatitis was established by intradermal IL-17A injection in STAT3 overexpressing mice. Transcriptome analyses showed that IL-17A exacerbated the pathological features of skin lesions in STAT3 overexpressing mice, which were highly consistent with those observed in psoriasis patients. Functional enrichment analysis revealed that the core differentially expressed genes were closely related to immune response, chemotaxis, and cornified envelope. The major KEGG enrichment pathways included IL-17 and Toll-like receptor signaling pathways.
Article
Multidisciplinary Sciences
Anissa Fries, Fanny Saidoune, Francois Kuonen, Isabelle Dupanloup, Nadine Fournier, Ana Cristina Guerra de Souza, Muzlifah Haniffa, Feiyang Ma, Johann E. Gudjonsson, Lennart Roesner, Yang Li, Thomas Werfel, Curdin Conrad, Raphael Gottardo, Robert L. Modlin, Jeremy Di Domizio, Michel Gilliet
Summary: This study establishes the role of interleukin 26 (IL-26) in the generation of IL-17A producing Th17 CD4(+) T cells, indicating its involvement in the pathogenic response of psoriasis. IL-26-producing cells infiltrate psoriatic skin and promote their own differentiation into IL17A-producing T(H)17 cells through epithelial crosstalk involving paracrine production of TGF-& beta;1.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Tithi Roy, Sergette Banang-Mbeumi, Samuel T. Boateng, Emmanuelle M. Ruiz, Roxane-Cherille N. Chamcheu, Lin Kang, Judy A. King, Anthony L. Walker, Bolni Marius Nagalo, Konstantin G. Kousoulas, Stephane Esnault, Shile Huang, Jean Christopher Chamcheu
Summary: Fisetin has potential therapeutic effects on refractory psoriasis by inhibiting abnormal immune response, promoting keratinocyte differentiation and autophagy, and reducing disease inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dermatology
Burak Aksan, Aysegul Basak Akadam-Teker
Summary: The TT genotype and T allele of IL-17A rs10484879 are associated with an increased risk of psoriasis in the Turkish population. This study suggests that IL-17A polymorphism may play a role in the pathogenesis of the disease. The IL-17A serum levels were also found to be associated with the genotype distribution.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2022)
Article
Immunology
Wenjuan Yang, Rukun He, Hao Qu, Wenwen Lian, Yue Xue, Tao Wang, Wenlong Lin, Peishuo Zhu, Meng Xia, Lihua Lai, Qingqing Wang
Summary: FXYD3 is upregulated in the lesional skin of psoriasis patients and mice with IMQ-induced psoriasis. It promotes IL-17A signaling in keratinocytes, leading to inflammation and the expression of proinflammatory factors, exacerbating psoriasis. Targeting FXYD3 could be a potential therapeutic approach for psoriasis.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tania Mariastella Caputo, Angela Maria Cusano, Menotti Ruvo, Anna Aliberti, Andrea Cusano
Summary: This study developed a thermal responsive drug delivery system using HSA particles loaded with SOR. The system demonstrated improved solubility and bioavailability of SOR through temperature-controlled drug release.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Giovanna Carpentieri, Chiara Leoni, Donatella Pietraforte, Serena Cecchetti, Egidio Iorio, Antonio Belardo, Daniele Pietrucci, Michela Di Nottia, Deborah Pajalunga, Francesca Megiorni, Laura Mercurio, Massimo Tatti, Simona Camero, Cinzia Marchese, Teresa Rizza, Valentina Tirelli, Roberta Onesimo, Rosalba Carrozzo, Sara Rinalducci, Giovanni Chillemi, Giuseppe Zampino, Marco Tartaglia, Elisabetta Flex
Summary: Germline-activating mutations in HRAS cause Costello syndrome, characterized by reduced postnatal growth. The dysregulated glucose intake and accelerated autophagic flux in primary fibroblasts of Costello syndrome patients can be rescued by inhibiting p38 and PI3K signaling. This mechanistic link between upregulated HRAS function and metabolic dysfunction in Costello syndrome provides potential therapeutic targets for reverting this condition.
HUMAN MOLECULAR GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Mariarosaria Conte, Rosanna Palumbo, Alessandra Monti, Elisabetta Fontana, Angela Nebbioso, Menotti Ruvo, Lucia Altucci, Nunzianna Doti
Summary: The AIF/CypA complex has lethal activity in rodent models of acute brain injury, but an AIF peptide has been found to restore cell survival and prevent brain injury. This study shows that the peptide can also protect against apoptosis in PD cells, suggesting the AIF/CypA complex as a potential target for developing new PD therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Marta Gogliettino, Emanuela Iaccarino, Carmela Fusco, Andrea Caporale, Menotti Ruvo, Gianna Palmieri, Ennio Cocca
Summary: APEH is a crucial protease involved in protein degradation, and the presence of sulphoxide-modified methionines plays an important role in its endopeptidase activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Luca Fania, Gaia Moretta, Flaminia Antonelli, Enrico Scala, Damiano Abeni, Cristina Albanesi, Stefania Madonna
Summary: Atopic dermatitis (AD) is a common chronic inflammatory skin disease characterized by intense itching and recurrent eczematous lesions. The pathogenesis of AD involves genetic background, skin barrier dysfunction, immune responses, and itch. Skin dehydration caused by filaggrin mutations is a key feature of AD. Cytokines play an important role in the pathogenesis of AD, and the cytokine patterns vary in patients with different clinical phenotypes. Understanding the correlation between cytokine patterns and clinical forms of AD is crucial for personalized therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Fernanda Scopelliti, Laura Mercurio, Caterina Cattani, Valentina Dimartino, Cristina Albanesi, Gianfranco Costanzo, Concetta Mirisola, Stefania Madonna, Andrea Cavani
Summary: PI3K p110 delta plays a crucial role in regulating the functions of human dendritic cells, and selective inhibition with seletalisib can reduce the maturation and antigen-presenting functions of DCs, leading to a decreased immune response and increased cell migration.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roberta Pacifico, Nunzio Del Gaudio, Guglielmo Bove, Lucia Altucci, Lydia Siragusa, Gabriele Cruciani, Menotti Ruvo, Rosa Bellavita, Paolo Grieco, Mauro F. A. Adamo
Summary: We have developed a synthetic method to produce two types of triazole compounds. Through virtual screening and experimental validation, one of the triazole compounds showed specific binding and inhibition towards KAT2A, laying the foundation for a new class of KAT2A inhibitors.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2022)
Article
Immunology
Martina Morelli, Marco Galluzzo, Claudia Scarponi, Stefania Madonna, Giovanni Luca Scaglione, Giampiero Girolomoni, Marina Talamonti, Luca Bianchi, Cristina Albanesi
Summary: Ustekinumab is an effective biologic therapy for psoriasis, but patient responses vary. This study identified 12 SNPs associated with excellent response to ustekinumab and found that HLA-C gene variations could help determine which patients would benefit from anti-IL-12/IL-23 therapy.
Article
Chemistry, Analytical
Sarassunta Ucci, Sara Spaziani, Giuseppe Quero, Patrizio Vaiano, Maria Principe, Alberto Micco, Annamaria Sandomenico, Menotti Ruvo, Marco Consales, Andrea Cusano
Summary: Lab-on-fiber (LoF) optrodes offer advantages for real-time and label-free detection of biomarkers. Improving immobilization strategies using hinge carbohydrates significantly enhances the detection capability. By optimizing and validating the LoF platform, detection of the cancer-related biomarker Cripto-1 has been greatly improved.
Review
Biochemistry & Molecular Biology
Nicole Balasco, Carlo Diaferia, Elisabetta Rosa, Alessandra Monti, Menotti Ruvo, Nunzianna Doti, Luigi Vitagliano
Summary: Amyloid aggregation is a widespread process involving proteins and peptides with various molecular complexities and amino acid compositions. The supramolecular organization of these aggregates generates cross-beta structures, which exhibit special mechanical and spectroscopic properties. One of the intriguing properties is the emission of intrinsic and label-free fluorescence in regions of the electromagnetic spectrum that are typically forbidden in polypeptide chains. This phenomenon has attracted significant interest in the fields of basic and applied science, although its physical basis is not well understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alessandra Monti, Luigi Vitagliano, Andrea Caporale, Menotti Ruvo, Nunzianna Doti
Summary: Protein-protein interfaces play crucial roles in pathophysiological pathways and are important targets for therapeutic compounds. However, identifying binding sites on protein surfaces and developing modulators of protein-protein interactions remain challenging due to their dynamic nature. Various strategies, including structural, computational, and combinatorial approaches, have been developed to characterize PPIs. Peptides are particularly potent and selective for inhibiting PPIs. This review discusses the impact of chemical peptide libraries in medicinal chemistry, focusing on recent applications, and explores the role of this methodology in the era of predictive approaches based on artificial intelligence in structural biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Alessia Ruggiero, Emanuela Iaccarino, Angela Oliver, Flavia Squeglia, Miguel Moreira, Luciana Esposito, Menotti Ruvo, Rita Berisio
Summary: CD59 is an abundant protein in humans that protects cells from damage by inhibiting the complement system. It inhibits the assembly of the Membrane Attack Complex (MAC) and is incorporated by pathogenic viruses like HIV-1 to escape complement-mediated virolysis. CD59 is also overexpressed in cancer cells to resist complement attack. In this study, bioinformatics and computational tools were used to identify CD59 interactions and design paratope-mimicking peptides that target CD59.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Jwala P. Sivaccumar, Emanuela Iaccarino, Angela Oliver, Maria Cantile, Pierpaolo Olimpieri, Antonio Leonardi, Menotti Ruvo, Annamaria Sandomenico
Summary: Drug development has shifted toward personalized treatments targeting specific therapeutic targets in individual patients. Monoclonal antibodies (mAbs) and their surrogates, such as Fabs, are increasingly used for their ability to bind and inhibit therapeutic targets. In this study, a recombinant humanized Fab of a murine mAb (3D1) was prepared and characterized, showing potential for targeting Nodal-positive cancer cells.
Article
Biophysics
A. M. Cusano, G. Quero, P. Vaiano, P. Cicatiello, M. Principe, A. Micco, M. Ruvo, M. Consales, A. Cusano
Summary: The study developed a label-free Lab-on-Fiber biosensing platform for highly sensitive detection of the major circulating form of vitamin D, 25(OH)D3. The platform combines plasmonic metasurfaces with optical fibers to enable direct and sensitive detection of 25(OH)D3 in clinically relevant ranges. This platform has the potential to be used in developing low-cost, fast, simple, and ready-to-use Point-of-Care diagnostic devices.
BIOSENSORS & BIOELECTRONICS
(2023)