Analytical techniques for characterization of biological molecules – proteins and aptamers/oligonucleotides

Fragment-Based Discovery of Pyrimido[1,2-b]indazole PDE10A Inhibitors

(2018) Ayaka Chino et al.   CHEMICAL & PHARMACEUTICAL BULLETIN

Synthesis, crystal structure and biological evaluation of a new dasatinib copper(II) complex as telomerase inhibitor

(2018) Qi-Pin Qin et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

X-ray crystal structure guided discovery of new selective, substrate-mimicking sirtuin 2 inhibitors that exhibit activities against non-small cell lung cancer cells

(2018) Ling-Ling Yang et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1

(2018) Wesley Tucker et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Structure-Based Discovery of a Series of 5H-Pyrrolo[2,3-b]pyrazine FGFR Kinase Inhibitors

(2018) Alan Jiang et al.   MOLECULES

Crystal structures of the gastric proton pump

(2018) Kazuhiro Abe et al.   NATURE

Aptamers in the Therapeutics and Diagnostics Pipelines

(2018) Harleen Kaur et al.   Theranostics

Recent developments in cell-SELEX technology for aptamer selection

(2018) Harleen Kaur   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Proteomics Analysis of Blood Serums from Alzheimer’s Disease Patients Using iTRAQ Labeling Technology

(2017) Liming Shen et al.   JOURNAL OF ALZHEIMERS DISEASE

Structure of the full-length glucagon class B G-protein-coupled receptor

(2017) Haonan Zhang et al.   NATURE

Differentially expressed proteins underlying childhood cortical dysplasia with epilepsy identified by iTRAQ proteomic profiling

(2017) Lu Qin et al.   PLoS One

Selection, characterization, and application of DNA aptamers for detection of Mycobacterium tuberculosis secreted protein MPT64

(2017) Marzhan Sypabekova et al.   TUBERCULOSIS

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis

(2017) Rui Niu et al.   PLoS One

Protein signature in cerebrospinal fluid and serum of Alzheimer’s disease patients: The case of apolipoprotein A-1 proteoforms

(2017) Chiara Fania et al.   PLoS One

Fluorescence-Activated Cell Sorting of Human l-asparaginase Mutant Libraries for Detecting Enzyme Variants with Enhanced Activity

(2016) Christos S. Karamitros et al.   ACS Chemical Biology

Aptamer-based ‘point-of-care testing’

(2016) Subash C.B. Gopinath et al.   BIOTECHNOLOGY ADVANCES

Triple SILAC quantitative proteomic analysis reveals differential abundance of cell signaling proteins between normal and lung cancer-derived exosomes

(2016) David J. Clark et al.   Journal of Proteomics

Therapy-Related Changes in the Serum Proteome Patterns of Early Stage Breast Cancer Patients with Different Outcomes

(2016) Walaszczyk Anna et al.   PROTEIN AND PEPTIDE LETTERS

G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA

(2016) Regina Stoltenburg et al.   Scientific Reports

iTRAQ-Based Proteomics Investigation of Aqueous Humor from Patients with Coats' Disease

(2016) Qiong Yang et al.   PLoS One

iTRAQ-based proteomic analysis of plasma reveals abnormalities in lipid metabolism proteins in chronic kidney disease-related atherosclerosis

(2016) Magdalena Luczak et al.   Scientific Reports

SILAC for biomarker discovery

(2015) Gunnar Dittmar  et al.   Proteomics Clinical Applications

Development and Application of an ELISA for the Detection of Porcine Deltacoronavirus IgG Antibodies

(2015) Anil Thachil et al.   PLoS One

Quantitative Analysis of Differential Proteome Expression in Bladder Cancer vs. Normal Bladder Cells Using SILAC Method

(2015) Ganglong Yang et al.   PLoS One

Monitoring the VEGF level in aqueous humor of patients with ophthalmologically relevant diseases via ultrahigh sensitive paper-based ELISA

(2014) Min-Yen Hsu et al.   BIOMATERIALS

Urine 2DE proteome analysis in healthy condition and kidney disease

(2014) Marta Martin-Lorenzo et al.   ELECTROPHORESIS

Flow cytometric quantification and characterization of intracellular protein aggregates in yeast

(2014) Ayala Shiber et al.   Prion

¹H NMR-based metabolic profiling of human rectal cancer tissue

(2013) Huijuan Wang et al.   Molecular Cancer

Investigating the Antiproliferative Activity of High Affinity DNA Aptamer on Cancer Cells

(2013) Harleen Kaur et al.   PLoS One

Open-Channel Structures of the Human Glycine Receptor α1 Full-Length Transmembrane Domain

(2013) David D. Mowrey et al.   STRUCTURE

Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS–PAGE coupled with nanoLC–ESI–MS/MS bottom-up proteomics

(2012) Cristina C. Clement et al.   Journal of Proteomics

Quantification of theN-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples

(2012) Paul J. Boersema et al.   MOLECULAR & CELLULAR PROTEOMICS

Probing High Affinity Sequences of DNA Aptamer against VEGF165

(2012) Harleen Kaur et al.   PLoS One

Agarose and polyacrylamide gel electrophoresis methods for molecular mass analysis of 5- to 500-kDa hyaluronan

(2011) Shardul Bhilocha et al.   ANALYTICAL BIOCHEMISTRY

Specific Sorting of Single Bacterial Cells with Microfabricated Fluorescence-Activated Cell Sorting and Tyramide Signal Amplification Fluorescence in Situ Hybridization

(2011) Chun H. Chen et al.   ANALYTICAL CHEMISTRY

Paper-Based ELISA

(2010) Chao-Min Cheng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker

(2010) Un-Beom Kang et al.   BMC CANCER

Serum protein biomarkers screening in HCC patients with liver cirrhosis by ICAT-LC-MS/MS

(2010) Xiaonan Kang et al.   JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY

Selection and Characterization of DNA Aptamers for Egg White Lysozyme

(2010) Dinh T. Tran et al.   MOLECULES

Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures

(2010) Günter Mayer et al.   Nature Protocols

Quantitative and Temporal Proteome Analysis of Butyrate-treated Colorectal Cancer Cells

(2008) Hwee Tong Tan et al.   MOLECULAR & CELLULAR PROTEOMICS

Perspectives on NMR in drug discovery: a technique comes of age

(2008) Maurizio Pellecchia et al.   NATURE REVIEWS DRUG DISCOVERY

Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques

(2008) Rudolf Amann et al.   NATURE REVIEWS MICROBIOLOGY