Review
Virology
Matias L. Pidre, Paula N. Arrias, Leslie C. Amoros C. Morales, Victor Romanowski
Summary: This article reviews the technological applications of insect-specific enveloped baculoviruses. It summarizes the natural cycle of the virus in larvae and cell culture and how it can be exploited. It discusses various applications of baculovirus-based protein expression systems (BEVS) in the pharmaceutical and biotechnological industry. Finally, it extensively describes different gene therapy strategies based on baculoviruses for the treatment of various diseases.
Review
Biotechnology & Applied Microbiology
Alexandra Marisa Targovnik, Jorge Alejandro Simonin, Gregorio Juan Mc Callum, Ignacio Smith, Franco Uriel Cuccovia Warlet, Maria Victoria Nugnes, Maria Victoria Miranda, Mariano Nicolas Belaich
Summary: Baculoviruses, as insect pathogens, have been widely used in biotechnological applications with various biological engineering features, including pest control, recombinant protein expression systems, mammalian gene delivery, and antigenic protein display systems. BEVS and BacMam technologies, due to their ease of use and availability as commercial systems, have been widely introduced in academia and the pharmaceutical industry as essential tools for developing biological products.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Genetics & Heredity
Cintia N. Parsza, Diego L. M. Gomez, Jorge A. Simonin, Mariano Nicolas Belaich, Pablo D. Ghiringhelli
Summary: The study demonstrated that the budded virions of Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) can transduce and support the expression of delivered genes in mammalian cells but do not replicate. Therefore, AgMNPV has the potential to be used as an alternative to non-infectious viruses in humans for exploring new approaches in gene therapy and other applications based on the use of mammalian cells.
CURRENT GENE THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Wenjun Guo, Mengmeng Wang, Lei Chen
Summary: The BacMam system, utilizing baculovirus as a delivery tool, is widely used for recombinant production of eukaryotic proteins in mammalian cells. In this study, a BacMam vector (pBMCL1) was developed, which allows easy tracking of virus production, enhances infection efficiency in mammalian cells, reduces unwanted transcription of toxic genes in insect cells, and enables the co-expression of multiple proteins using a single virus. The successful application of the pBMCL1 vector in expressing homo-tetrameric human TRPC3 channel and hetero-octameric KATP channel is demonstrated.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Romina J. Bevacqua, Xiaoqing Dai, Jonathan Y. Lam, Xueying Gu, Mollie S. H. Friedlander, Krissie Tellez, Irene Miguel-Escalada, Silvia Bonas-Guarch, Goutham Atla, Weichen Zhao, Seung Hyun Kim, Antonia A. Dominguez, Lei S. Qi, Jorge Ferrer, Patrick E. MacDonald, Seung K. Kim
Summary: The successful genome editing in primary human islets using CRISPR and Cas9 revealed genetic and regulatory mechanisms associated with diabetes pathogenesis. Targeting regulatory elements associated with T2D susceptibility could provide insight into diabetes risk and genetic susceptibility.
NATURE COMMUNICATIONS
(2021)
Article
Virology
Eduard Puente-Massaguer, Byron Cajamarca-Berrezueta, Aleix Volart, Irene Gonzalez-Dominguez, Francesc Godia
Summary: This study explores the production of Gag VLPs with the BacMam expression system in a suspension culture of HEK293 cells and determines the optimal culture conditions. The results show that high cell transduction efficiency and VLP production can be achieved under optimal conditions, and the characteristics of the produced VLPs are validated using super-resolution fluorescence microscopy. Evidence of different pathways for BacMam transduction is also provided. This work lays the foundation for future research aiming to scale up the BacMam baculovirus system as an alternative strategy for VLP production.
Article
Multidisciplinary Sciences
Soujanya S. Karanth, Shuofei Sun, Huanjing Bi, Kaiming Ye, Sha Jin
Summary: A newly discovered angiopoietin signal promotes the generation of mature islets from human induced pluripotent stem cells (iPSCs). The stimulated islets show enhanced glucose responsiveness and regulated insulin exocytosis through actin-filament polymerization and depolymerization. Angiopoietins act as signaling molecules to endorse in vitro islet development from iPSCs.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Muhammed Muhsin Varikkodan, Chun-Chung Chen, Tzong-Yuan Wu
Summary: Chikungunya virus (CHIKV) is a global threat due to its high contagion rate and lack of effective remedies. Two BEVS-based approaches were developed to screen anti-CHIKV drugs, with one involving cell fusion in insect cells and the other using a replicon system in human cells to identify potential inhibitory compounds. The results suggest that BEVS could be a valuable tool for drug screening against CHIKV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Ebrahim Kord, Farzin Roohvand, Jean Dubuisson, Thibaut Vausselin, Hosein Nasr Azadani, Abolfazl Keshavarz, Ahmad Nejati, Katayoun Samimi-Rad
Summary: The study demonstrated a BacMam virus-based surface display for subtype-1a HCV gpE2, showing proper expression and incorporation, and successful induction of strong humoral and cellular immune responses in immunized mice. The BacMam virus-based surface display for gpE2 offers a promising strategy for the development of an efficient HCV vaccine.
INFECTIOUS AGENTS AND CANCER
(2021)
Article
Biotechnology & Applied Microbiology
Anna Voznesenskaya, Per-Olof Berggren, Erwin Ilegems
Summary: This study characterized the transduction performance of AAV serotype 8 (AAV8) and human adenovirus type 5 (AdV5) during pseudoislet formation. The results provide a reference for future studies using AAVs for gene transfer to islet cell organoids and facilitate the development of high-quality engineered pseudoislets for clinical transplantation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Diti Chatterjee Bhowmick, Lydia Burnett, Zhanar Kudaibergenova, Aleksandar M. Jeremic
Summary: The study found significant accumulation of hIAPP in the nucleus under high glucose stress conditions, with the activation of the hIAPP promoter mediated by the transcription factor FoxA2. Despite the lack of adverse effects from nucleolar accumulation of hIAPP, intact nucleolar organization and function were essential for hIAPP expression under normal and ER-stress conditions.
BIOCHEMICAL JOURNAL
(2021)
Article
Microbiology
Lionel Galibert, Aurelien Jacob, Adrien Savy, Yohann Dickx, Delphine Bonnin, Christophe Lecomte, Lise Rivollet, Peggy Sanatine, Marjorie Boutin Fontaine, Christine Le Bec, Otto-Wilhelm Merten
Summary: Large-scale manufacturing of rAAV remains a challenge, with the Monobac system showing higher yields of rAAV vectors and lower DNA contamination compared to dual baculovirus systems.
Article
Biotechnology & Applied Microbiology
Shan-Ko Tsai, Yu-Lin Hsu, Der-Jiang Chiao, Pei-Yun Shu, Hui-Tsu Lin, Shu-Fen Chang, Hui-Chung Lin, Shih-Hsin Huang, Chun-Hsiung Wang, Chia-Chu Hsiung, Chang-Chi Lin, Tzong-Yuan Wu, Szu-Cheng Kuo
Summary: This study describes the production of novel CHIKV virus-like particles (VLPs) from mosquitoes using a Baculovirus/Mosquito system. The VLPs showed potential as an antigen for serological detection and vaccine development against CHIKV infection, as they were able to induce neutralizing antibody responses and specific antibody production in mice.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Veterinary Sciences
Yuhua Gao, Weijun Guan, Chunyu Bai
Summary: In this study, we isolated and cultured pancreatic ductal cells from canines and successfully induced their differentiation into functional pancreatic beta cells. The differentiated cells expressed insulin and glucagon, synthesized insulin granules, and secreted insulin in response to glucose stimuli.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Cell Biology
Marta Perez-Frances, Maria Valentina Abate, Delphine Baronnier, Philipp E. Scherer, Yoshio Fujitani, Fabrizio Thorel, Pedro L. Herrera
Summary: This study investigates the developmental dynamics of pancreatic islet endocrine cell types using murine models. The researchers find that adult islet cells originate from embryonic hormone-expressing cells and there is no evidence of islet cell differentiation from precursor cells after birth. The study also reveals specific patterns of hormone gene activation and switching during islet morphogenesis.
Article
Virology
Farheen Raza, Joanna F. McGouran, Benedikt M. Kessler, Robert D. Possee, Linda A. King
JOURNAL OF VIROLOGY
(2017)
Article
Virology
Ines Beperet, Sarah L. Irons, Oihane Simon, Linda A. King, Trevor Williams, Robert D. Possee, Miguel Lopez-Ferber, Primitivo Caballero
JOURNAL OF VIROLOGY
(2014)
Article
Multidisciplinary Sciences
Hui Wang, Jiazheng Xie, Tim G. Shreeve, Jinmin Ma, Denise W. Pallett, Linda A. King, Robert D. Possee
Article
Microbiology
Leo P. Graves, Louise C. Hughes, Sarah L. Irons, Robert D. Possee, Linda A. King
Article
Virology
Aurelie Hinsberger, Benoit Graillot, Christine Blachere Lopez, Sylvie Juliant, Martine Cerutti, Linda A. King, Robert D. Possee, Franck Gallardo, Miguel Lopez Ferber
Article
Engineering, Chemical
Carina Bannach, Daniel Ruiz Buck, Genna Bobby, Leo P. Graves, Sainan Li, Adam C. Chambers, Elizabeth Gan, Raquel Arinto-Garcia, Robert D. Possee, Linda A. King
Summary: Autographa californica nucleopolyhedrovirus is an efficient expression vector for recombinant proteins in insect cells. The study found that using the AUA codon may slightly improve protein yield, while the AUU codon may lead to leaky translation.
Article
Biochemical Research Methods
Stuart D. Dowall, Leo P. Graves, Emma Kennedy, Victoria A. Graham, Riyadh A. Alakeely, Adam Chambers, Robert D. Possee, Linda A. King, Roger Hewson
Summary: Humanised antibodies targeting Crimean-Congo Haemorrhagic virus (CCHFV) are crucial in meeting diagnostic standards and expanding knowledge about its distribution. Comparisons between traditional mouse hybridoma and humanisation methods demonstrate the former generates higher affinity antibodies, but still depends on animal usage. This study provides valuable information for developing standardised controls and reducing animal usage for future applications.
JOURNAL OF IMMUNOLOGICAL METHODS
(2023)
Review
Biochemistry & Molecular Biology
Robert D. Possee, Adam C. Chambers, Leo P. Graves, Mine Aksular, Linda A. King
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Robert Possee
GENETIC ENGINEERING & BIOTECHNOLOGY NEWS
(2011)