Article
Engineering, Biomedical
Caixia Sun, Xinyu Zhou, Cong Liu, Shuyue Deng, Yuhan Song, Jun Yang, Jianjun Dai, Yanmin Ju
Summary: Researchers have developed an integrated therapeutic and preventive nanozyme-based microneedle (Fe2C/GOx@MNs) for treating biofilm-infected diabetic wounds. The dissolvable tips of the microneedles can release Fe2C nanoparticles/glucose oxidase (GOx) in the active regions of the biofilm, improving tissue and biofilm permeability and achieving efficient biofilm elimination. The chitosan backing layer acts as a physical barrier and prevents bacterial reinvasion during wound healing. The study demonstrates the promising clinical application of Fe2C/GOx@MNs in biofilm-infected wound healing.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biophysics
Karan Razdan, Shashi Kanta, Ekta Chaudhary, Seema Kumari, Deepak Kumar Rahi, Ashok Kumar Yadav, Vivek Ranjan Sinha
Summary: The study developed and characterized levofloxacin-loaded clove oil nanoscale emulgel and evaluated its therapeutic efficacy in Pseudomonas aeruginosa biofilm-infected burn wounds in mice. The results showed that the emulgel dispersed biofilms, closed wounds, promoted re-epithelialization and collagen deposition, and cleared the infection within 7 days, indicating its effectiveness in treating biofilm-infected burn wounds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Environmental
Mingming Qin, Yanqiu Guo, Feifei Su, Xiaopeng Huang, Qiuping Qian, Yunlong Zhou, Jingye Pan
Summary: The development of functional medical dressings with mechanical designability is essential for tissue repair. However, existing liquid or gel dressings have limitations in terms of mechanical properties and antimicrobial biofilm, hindering their applications in emergency cases. In this study, a nanocomposite hydrogel dressing was developed, incorporating gold nanorods and Ca2+ into polyacrylic acid and polyvinyl alcohol, resulting in a double network structure with high toughness and excellent fatigue resistance. The hydrogel dressings exhibited good biocompatibility, self-healing function, and efficient elimination of bacterial biofilm. Moreover, the dressing was found to promote wound healing in vivo.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Hanif Haidari, Richard Bright, Sanjay Garg, Krasimir Vasilev, Allison J. Cowin, Zlatko Kopecki
Summary: The development of highly potent ultrasmall AgNPs combined with a biocompatible hydrogel has shown promising results in eliminating mature biofilm infections in vivo and promoting wound healing. The treatment exhibited superior antibacterial efficacy and improved wound closure, re-epithelization, and collagen deposition compared to conventional silver-based treatments.
Review
Environmental Sciences
Jile Fu, Yiyi Xu, Eric J. Arts, Zhengyu Bai, Zhongwei Chen, Ying Zheng
Summary: The transmission of viral infections via aerosol is a serious threat to public health, and non-thermal plasma technology has gained interest for its highly effective disinfection and flexible operation.
Article
Biotechnology & Applied Microbiology
Hye Rin Kim, Keunbada Son, Young-Tak Son, Yong-Gun Kim, Kyu-Bok Lee, Seung Cheol Lee, Jo-Young Suh, Jae Mok Lee
Summary: This study examined the healing patterns of dental diode lasers and a steel scalpel in periodontal surgery. The results showed that laser-treated wounds had slower healing rates compared to scalpel-treated wounds, but no significant difference was observed in histological and immunohistochemical analysis between the laser and scalpel groups.
BIOENGINEERING-BASEL
(2022)
Article
Nanoscience & Nanotechnology
Dengyan Wu, Dong Wei, Maotao Du, Song Ming, Qian Ding, Ranjing Tan
Summary: In this study, novel chitosan and poly (D,L-lactide-co-glycolide) nanoparticles loaded with benzalkonium bromide (BZK) were developed for wound healing after MRSA infection. These nanoparticles exhibited significant antibacterial activity, anti-biofilm properties, and the ability to damage bacterial cell walls, indicating their potential in promoting skin wound healing. These findings suggest a new avenue for nanomedicine development in wound treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Walker D. D. Short, Oluyinka O. O. Olutoye, Benjamin W. W. Padon, Umang M. M. Parikh, Daniel Colchado, Hima Vangapandu, Shayan Shams, Taiyun Chi, Jangwook P. P. Jung, Swathi Balaji
Summary: Impaired wound healing is a significant financial and medical burden. Disruptions in ECM deposition, structure, and composition lead to impaired healing in diseased states, such as in diabetes. Valid measures include lack of bacterial contamination, good tissue perfusion, and reduced mechanical injury and strain. This review discusses bioengineering advances in detecting biologic and physiologic factors, visualizing and modeling the ECM, and efficiently evaluating wound data. It focuses on bioelectronics and biologic interfaces for real-time sensing and actuation, advanced imaging techniques, and computational modeling. These advancements have the potential to improve healing outcomes and guide decision-making in wound care.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Medicine, Research & Experimental
Aakansha Giri Goswami, Somprakas Basu, Tuhina Banerjee, Vijay Kumar Shukla
Summary: The biofilm, a community of microorganisms protected by a self-produced matrix, plays a vital role in infection and antimicrobial resistance. The formation, growth, and resistance mechanism of biofilm are still not fully understood. Although there have been studies on anti-biofilm and antimicrobial therapeutics, the lack of a clear clinical standard calls for the translation of laboratory research into novel bedside strategies. Biofilm is responsible for faulty wound healing and chronic wounds. Understanding its interaction with wounds and developing reproducible anti-biofilm measures in a clinical setting is the current challenge.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Sourbh Suren Garg, Rupal Dubey, Sandeep Sharma, Ashish Vyas, Jeena Gupta
Summary: Chronic wounds in diabetes mellitus patients are difficult to heal due to high glucose levels, oxidative stress, and biofilm-associated microbial infection. Conventional antibiotic therapies are ineffective in clinical settings as antibiotics cannot penetrate the microbial biofilm matrix. Therefore, finding safer alternatives to reduce chronic wound infection is urgently needed. Using biological-macromolecule based nano-delivery systems to inhibit biofilm formation offers advantages such as higher drug loading efficiency, sustained drug release, enhanced drug stability, and improved bioavailability. This review discusses the pathogenesis, microbial biofilm formation, and immune response of chronic wounds, as well as the potential of macromolecule-based nanoparticles as wound healing therapies to reduce mortality associated with chronic wound infections.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
M. C. Linju, M. R. Rekha
Summary: The present study developed a L-proline conjugated chitosan scaffold for wound healing. The scaffold was characterized by various tests and showed no cytotoxicity. In vitro experiments demonstrated that the modified scaffold promoted wound closure and collagen deposition. These findings suggest that the L-proline conjugated scaffold has potential as a wound dressing to improve wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Dermatology
Fergus Watson, Rui Chen, Steven L. Percival
Summary: This study aimed to evaluate the antibacterial properties of commercially available wound dressings, with different material composition and antibacterial agents, against various in vitro microbial and biofilm models. The results showed that controlled-release iodine foam and silver-impregnated carboxymethylcellulose (CMC) wound dressings had better biofilm management properties compared to methylene blue and gentian violet-containing foam dressings. The controlled-release iodine foam and silver-impregnated CMC materials effectively prevented the dissemination of Pseudomonas aeruginosa.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Xanthe L. L. Strudwick, Jason D. D. Whittle, Allison J. J. Cowin, Louise E. E. Smith
Summary: Fundamental knowledge about cell-surface interactions can be used in the development of wound dressings and scaffolds that promote wound healing. Plasma-coating wound dressings with acid-functionalised monomers or amine functionalisation can enhance cell adhesion, proliferation, and migration. In an in vivo study on mice, dressings coated with allylamine showed increased wound healing rate and reduced wound area, while prolonged treatment with allylamine-functionalised dressings may impair healing. Acrylic acid functionalisation did not improve early wound healing, but promoted collagen deposition at a later stage. This suggests that plasma polymerisation can lead to the development of new dressings that enhance wound closure by directing cell behavior, but a timed approach may be necessary for their application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Min Gyu Song, Pantu Kumar Roy, Eun Bi Jeon, So Hee Kim, Min Soo Heu, Jung-Suck Lee, Jae-Suk Choi, Jin-Soo Kim, Shin Young Park
Summary: This study investigated the effects of plasma on Listeria monocytogenes mixed-culture biofilms formed on various surfaces. Plasma was found to disrupt intercellular contact and induce cell decomposition, thereby preventing biofilm formation. The results suggest that plasma can be used as an alternative non-heating sterilization technology to reduce biofilm formation on bacterial targets in the food industry.
Article
Engineering, Environmental
Junyao Song, Haoyu Chen, Yaqian Lv, Wanqun Yang, Fenglang Zhang, Tianyi Wang, Danping Liu, Yingshan Qu, Lei Han, Jun Fu, Xiaoying Kong
Summary: This study developed a hybrid single atom nanozyme material composed of Zn single-atom catalysts and copper peroxide, which exhibited superior antibacterial properties and therapeutic effects for the treatment of biofilm-infected wounds.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Jaroslav Julak, Eva Vankova, Marketa Valkova, Petra Kasparova, Jan Masak, Vladimir Scholtz
FOLIA MICROBIOLOGICA
(2020)
Article
Biotechnology & Applied Microbiology
Martina Paldrychova, Eva Vankova, Petra Kasparova, Eliska Sembolova, Olga Matatkova, Jan Masak, Vladimir Scholtz, Jaroslav Julak
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2020)
Article
Microbiology
Jaroslav Lux, Radim Dobias, Ivana Kuklova, Radek Litvik, Vladimir Scholtz, Hana Souskova, Josef Khun, Jakub Mrazek, Michaela Kantorova, Pavla Jaworska, Tana Prejdova, Jana Snuparkova, Petr Hamal, Jaroslav Julak
Review
Microbiology
Mohammad Yousefi, Masoud Aman Mohammadi, Maryam Zabihzadeh Khajavi, Ali Ehsani, Vladimir Scholtz
Summary: Novel non-thermal technologies such as non-thermal plasma, electron beam radiation, and pulsed light show promising results in degrading mycotoxins, with some studies reporting efficiency of over 90%. However, these technologies are still in the early stages of development for industrial applications.
Review
Plant Sciences
Bozena Sera, Vladimir Scholtz, Jana Jiresova, Josef Khun, Jaroslav Julak, Michal Sery
Summary: The legumes are the second most important agricultural crop, providing vegetable proteins and oils for human consumption. Non-thermal plasma treatment is an effective method for surface microbial inactivation and seed stimulation. This review summarizes information on the effects of NTP treatment on legume seeds and plants, including seed germination, microbial decontamination, seed wettability, and metabolic activity, as well as discussing the potential benefits in reducing phytotoxic diseases and seed dormancy.
Article
Microbiology
Eliska Lokajova, Jaroslav Julak, Josef Khun, Hana Souskova, Radim Dobias, Jaroslav Lux, Vladimir Scholtz
Summary: The study revealed that sensitivity to non-thermal plasma is not a fixed characteristic of fungal species, but varies among different strains. Significant differences were observed in the inactivation dynamics of various dermatophytes exposed to plasma, indicating that sensitivity is strain-specific rather than species-specific.
Article
Biochemistry & Molecular Biology
Josef Khun, Anna Machkova, Petra Kasparova, Myron Klenivskyi, Eva Vankova, Pavel Galar, Jaroslav Julak, Vladimir Scholtz
Summary: Non-thermal plasma (NTP) is a promising tool for inhibiting bacterial, viral, and fungal diseases. Two types of NTP sources based on cometary and point-to-ring discharges were studied and compared. The cometary discharge is less stable but forms smaller and rapidly emerging inhibition zones, while the point-to-ring discharge is more stable but provides larger affected but often not completely inhibited zones.
Article
Food Science & Technology
Vladimir Scholtz, Jana Jiresova, Bozena Sera, Jaroslav Julak
Summary: NTP is a new and safe microbicidal agent with significant beneficial effects on cereals and products, showing high potential for future practical use in decontamination and disinfection.
Article
Plant Sciences
Jana Jiresova, Vladimir Scholtz, Jaroslav Julak, Bozena Sera
Summary: Recently, the use of low-temperature plasmas and plasma-activated water (PAW) in biological research has gained much attention. This article reviews the composition and properties of PAW prepared by different methods, and finds no significant difference between PAW prepared by plasma treatment and that prepared by mixing the active ingredients. The research also shows that PAW promotes germination and growth of wheat grains, and effectively decontaminates bacteria and yeast.
Article
Chemistry, Physical
Pavel Galar, Tomas Popel, Josef Khun, Irena Matulkova, Ivan Nemec, Katerina Dohnalova Newell, Alena Michalcova, Vladimir Scholtz, Katerina Kusova
FARADAY DISCUSSIONS
(2020)
