Article
Microbiology
Yiling Fan, Shujuan Wang, Minghui Song, Liangliang Zhou, Chengzhi Liu, Yan Yang, Shuijing Yu, Meicheng Yang
Summary: This study mined specific proteins and their protein-coding genes for the detection of Burkholderia cepacia Complex (BCC) bacteria. A real-time recombinase polymerase amplification (rt-RPA) assay was developed for rapid screening of pharmaceutical and personal care products. The results showed high specificity and sensitivity of the assay, with the ability to detect BCC bacteria in a short time.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Gaihua Cao, Jiangbo Dong, Xiaolong Chen, Peng Lu, Yifan Xiong, Lan Peng, Jiawei Li, Danqun Huo, Changjun Hou
Summary: In this study, a strategy (MPT-Cas12a/13a) was developed that combines CRISPR/Cas12a and Cas13a for simultaneous detection of CaMV35S and T-nos. This strategy utilizes multiplex PCR and transcription to achieve simultaneous detection with different signals in the same space. The MPT-Cas12a/13a showed excellent sensitivity, with detection limits as low as 11 copies of T-nos and 13 copies of CaMV35S, and demonstrated outstanding specificity and anti-interference ability in actual sample analysis. Therefore, it has the potential to be used for the detection of GM crops.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Mengnan Fan, Jianru Yang, Xiaosu Wang, Yongjie Xu, Bing Li, Hui Yang, Qin Lu, Xun Min, Meirong Huang, Jian Huang
Summary: Neisseria gonorrhoeae is the only pathogen that causes gonorrhea. A developed detection method based on recombinase polymerase amplification (RPA) can eliminate false-positive signals and has the advantages of rapid performance, high sensitivity, and minimal dependency on equipment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Immunology
David M. Waag, Taylor B. Chance, Sylvia R. Trevino, Franco D. Rossi, David P. Fetterer, Kei Amemiya, Jennifer L. Dankmeyer, Susham S. Ingavale, Steven A. Tobery, Xiankun Zeng, Steven J. Kern, Patricia L. Worsham, Christopher K. Cote, Susan L. Welkos
Summary: Burkholderia mallei is a gram-negative animal pathogen causing glanders, a contagious and potentially fatal disease. Humans can also be infected, with a wide range of clinical forms, making treatment complicated. Developing effective medical countermeasures requires well-characterized animal models, with the African green monkey identified as optimal for acute glanders infection.
MICROBIAL PATHOGENESIS
(2021)
Article
Plant Sciences
Changfeng Li, Yuliang Ju, Pengfei Shen, Xun Wu, Le Cao, Benguo Zhou, Xiaoming Yan, Yuemin Pan
Summary: A novel RPA-LFD assay for the rapid visual detection of R. solanacearum was established in this study, showing high sensitivity to the pathogen. The assay, based on recombinase polymerase amplification and lateral-flow dipstick, targets the RipTALI-9 gene sequence and allows for quick and accurate diagnosis of bacterial wilt in tobacco plants.
Article
Microbiology
Emanuelle Baldo Gaspar, Lenita Ramires dos Santos, Andrea Alves do Egito, Maria Goretti dos Santos, Cynthia Mantovani, Juliana da Silva Gomes Rieger, Guilherme Augusto de Sousa Abrantes, Paula Adas Pereira Suniga, Julia de Mendonca Favacho, Ingrid Batista Pinto, Alessandra Figueiredo de Castro Nassar, Fernando Leandro dos Santos, Flabio Ribeiro de Araujo
Summary: This study assessed the virulence of a specific Burkholderia mallei strain isolated in Brazil in BALB/c mice, and found that the strain exhibited detectable virulence in mice, highlighting the importance of investigating the virulence mechanisms associated with glanders infection.
Article
Chemistry, Analytical
Chang Liu, Xuechun Yao, Chunlong Liu, Shengping You, Wei Qi, Mengfan Wang
Summary: The study established and optimized two lateral flow device based molecular assay systems, RPA-NFO-LFT and RPA-Cas12a-LFT, for the detection of Candida albicans (C. albicans) infection. Both systems showed good specificity and sensitivity, with a lower detection limit of 10(3) CFU ml(-1). RPA-Cas12a-LFT demonstrated higher accuracy for visual interpretation and better stability towards samples with serum nucleic acid interference compared to the conventional qPCR method.
ANALYTICAL METHODS
(2023)
Article
Microbiology
Paula A. Pereira Suniga, Cynthia Mantovani, Maria G. Santos, Juliana S. Gomes Rieger, Emanuelle B. Gaspar, Fernando Leandro dos Santos, Rinaldo A. Mota, Karla P. Chaves, Andrea A. Egito, Jose Carlos O. Filho, Alessandra F. Castro Nassar, Lenita Ramires dos Santos, Flabio R. Araujo
Summary: This study demonstrated the detection of Burkholderia mallei in equids with positive serology for glanders in all five geographic regions of Brazil using species-specific PCR and sequencing. The use of this method expands the possibility of strain isolation and epidemiological characterizations based on molecular information. The microbiological detection of B. mallei in cultures from asymptomatic equids raises the possibility of environmental elimination of the agent.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yanling Pang, Feng Cong, Xinheng Zhang, Hongxin Li, Yung-Fu Chang, Qingmei Xie, Wencheng Lin
Summary: A probe-based recombinase polymerase amplification assay was developed for rapid detection of Chlamydia psittaci. The test showed high sensitivity and accuracy in field applications, offering a promising approach for screening and monitoring outbreaks in domestic fowl populations.
Article
Immunology
Sheetal Saini, Harisankar Singha, Karuppusamy Shanmugasundaram, Bhupendra Nath Tripathi
Summary: Burkholderia mallei causes glanders, a highly fatal infectious disease in equines. This study found that infected equids exhibited strong humoral and cellular immune responses, suggesting the potential use of specific proteins as vaccine candidates.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Chih-Yuan Chiang, Yang Zhong, Michael D. Ward, Douglas J. Lane, Tara Kenny, Raysa Rosario-Acevedo, Brett P. Eaton, Sylvia R. Trevino, Taylor B. Chance, Meghan Hu, Patricia L. Worsham, David M. Waag, Richard T. Moore, Lisa H. Cazares, Christopher K. Cote, Yingyao Zhou, Rekha G. Panchal
Summary: Burkholderia mallei, the causative agent of glanders, poses a serious biological threat due to its infectivity, antibiotic resistance, and lack of approved vaccines. Studying host responses in non-human primates has revealed insights into the pathogenesis of glanders. Integration of proteomics data has led to the construction of a predictive protein-protein interaction network, shedding light on the molecular mechanisms underlying B. mallei infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Seda Ekici, Orhan Dudakli, Dilek Dulger, Maksut Murat Maden, Ayse Demirhan
Summary: The National Glanders Eradication Project successfully eradicated Glanders in our country between 2000-2001. However, in December 2019, 81 horses were culled in Turkiye due to the detection of an epidemic in horses in Buyukada. Glanders cases were also reported in horses in Usak and Bolu in 2019. The use of PCR for the reliable and fast detection of Burkholderia mallei in horses could prevent the future spread of infections in Turkiye.
ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
(2023)
Letter
Immunology
Harisankar Singha, Mandy C. Elschner, Praveen Malik, Sheetal Saini, Bhupendra N. Tripathi, Katja Mertens-Scholz, Hanka Brangsch, Falk Melzer, Raj K. Singh, Heinrich Neubauer
Summary: This study collected 10 Burkholderia mallei isolates from equids in 9 districts in India during glanders outbreaks from 2013 to 2016. Multilecus variable number tandem-repeat analysis revealed 7 outbreak area-related genotypes. The study emphasizes the utility of this analysis for epidemiological tracing of specific B. mallei isolates during outbreaks.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Leah M. Dignan, Rachelle Turiello, Tiffany R. Layne, Killian C. O'Connell, Jeff Hickey, Jeff Chapman, Melinda D. Poulter, James P. Landers
Summary: This proposed method offers a rapid and reliable approach for SARS-CoV-2 sample preparation and analysis, allowing RNA extraction in under 10 minutes and demonstrating good performance and compatibility with clinical samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Fisheries
Gen Li, Feng Cong, Weiyou Cai, Jinhui Li, Miaoli Wu, Li Xiao, Xiaoliang Hu, Weiwei Zeng, Dongsheng He
Summary: The RPA method can rapidly detect EHP within a short period of time, demonstrating high efficiency, sensitivity, and specificity for detecting parasitic shrimp.
AQUACULTURE REPORTS
(2021)
Article
Parasitology
Apoorva Saxena, Vijai Pal, Nagesh Kumar Tripathi, Ajay Kumar Goel
Article
Multidisciplinary Sciences
Nidhi Puranik, Manoj Kumar, N. Tripathi, V. Pal, A. K. Goel
DEFENCE SCIENCE JOURNAL
(2019)
Article
Biology
Nidhi Puranik, Vijai Pal, Nagesh Kumar Tripathi, Ajay Kumar Goel
Article
Biotechnology & Applied Microbiology
M. Kumar, N. Puranik, A. Varshney, N. Tripathi, V Pal, A. K. Goel
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Biochemical Research Methods
Rita Singh, Vijai Pal, N. K. Tripathi, A. K. Goel
MOLECULAR AND CELLULAR PROBES
(2020)
Article
Parasitology
Rita Singh, Vijai Pal, Manoj Kumar, N. K. Tripathi, A. K. Goel
Summary: Plague, caused by Yersinia pestis, is a zoonotic disease primarily found in rodents and transmitted to humans through flea bite. A PCR assay coupled with lateral flow strips has been developed for rapid and accurate detection of Y. pestis, showing high specificity and sensitivity, and eliminating the need for agarose gel electrophoresis for post amplification analysis.
Article
Biotechnology & Applied Microbiology
Swati Banger, Vijai Pal, N. K. Tripathi, A. K. Goel
Summary: A LAMP assay set targeting a chromosomal and two plasmid markers was developed in this study for rapid, sensitive detection of Bacillus anthracis, providing an important tool for clinical management and prevention of anthrax transmission.
FOLIA MICROBIOLOGICA
(2021)
Article
Biochemistry & Molecular Biology
Swati Banger, Vijai Pal, N. K. Tripathi, A. K. Goel
Summary: The PCR-LF assay targeting the cya gene of B. anthracis allows for fast and sensitive detection of as few as 500 copies of target DNA. It can detect as low as 10(3) and 10 CFU/mL of B. anthracis Sterne cells spiked in human blood, after 6 and 24 hours of enrichment, respectively.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Microbiology
Apoorva Saxena, Vijai Pal, Nagesh Kumar Tripathi, Ajay Kumar Goel
Summary: This study reports a rapid, specific, and sensitive method for detecting B. pseudomallei, a fatal and infectious disease. The method can detect very low concentrations of B. pseudomallei and does not cross-react with other bacterial strains.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2022)
