Article
Chemistry, Analytical
Fan Cai, Ningyuan Fang
Summary: The proposed method utilizes a commercial personal glucose meter for exosomes quantification, translating signals to achieve sensitive detection of exosomes, with potential application in clinical diagnosis for early detection of atherosclerosis.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Ruben R. G. Soares, Aleksandra Pettke, Agustin Robles-Remacho, Sahar Zeebaree, Sibel Ciftci, Marianna Tampere, Aman Russom, Marjo-Riitta Puumalainen, Mats Nilsson, Narayanan Madaboosi
Summary: This study demonstrates a highly sensitive Zika virus diagnostic method using padlock probes for isothermal amplification, combined with a microfluidic affinity chromatography enrichment platform. The method showed a positive correlation with RT-qPCR, validating its potential for cost-effective and simple point-of-care viral diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Xiaowen Cao, Feng Chen, Jing Xue, Yue Zhao, Min Bai, Yongxi Zhao
Summary: Here, we present a hierarchical DNA branch assembly-encoded fluorescent nanoladders for denoised and highly multiplexed signal amplification of single-cell transcripts. Our method achieves independent RNA-primed rolling circle amplification without nonspecific amplification and allows for programmable DNA branch assembly to encode virtual signals for visualizing numbers of targets. We demonstrate accurate RNA profiling in different cancer cells and reveal diverse localization patterns for spatial regulation of transcripts.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Yu Gu, Xuejiao Yang, Shanwen Hu, Chunxian Guo, Bo Chen, Cunxia Fan, Chang Ming Li
Summary: A novel glucometer-based microfluidic platform was developed for sensitively detecting cancer biomarker HER2. The assay uses rolling cycle amplification technology with a miniaturized personal glucose meter, on a small-sized microfluidic chip with high sensitivity and wide dynamic range, showing great potential for clinical diagnosis at home or in urban areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Jayeon Song, Yong Ju, Soohyun Kim, Hansol Kim, Hyun Gyu Park
Summary: In this study, we developed a palindromic hyperbranched rolling circle amplification (PH-RCA) reaction for ultrasensitive detection of microRNAs. The PH-RCA reaction significantly enhances the sensitivity and reduces the reaction time within 30 minutes, making it a promising technique for miRNA detection.
CHEMICAL COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Wenyan Jiang, Zhongping Chen, Jin Lu, Xue Ren, Yue Ma
Summary: This study developed a new detection method based on gold nanoparticles and CRISPR/Cas12a for highly sensitive detection of miRNA-143 as a noninvasive biomarker for prostate cancer diagnosis. The method exhibited outstanding specificity and sensitivity, and it is simple to operate, making it suitable for use in low-resource settings.
Article
Biophysics
Hwang-soo Kim, Naseem Abbas, Sehyun Shin
Summary: The study proposes an innovative method for detecting the new coronavirus using isothermal amplification of nucleic acids on a mesh with multiple microfluidic pores, with a detection limit of 0.7 aM. This approach offers rapid, easy, and effective detection of the target pathogen without the need for sophisticated equipment.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Xianming Jiang, Xiaoguang Pan, Wenchao Li, Peng Han, Jiaying Yu, Jing Li, Haoran Zhang, Wei Lv, Ying Zhang, Yulong He, Xi Xiang
Summary: This study found that the abundance of eccDNA was significantly higher in gastric cancer tissues compared to normal tissues. The eccDNA carrying oncogene-segments may contribute to the development of gastric cancer and facilitate cancer progression. Blocking the pathways for eccDNA generation could be a potential therapeutic strategy for gastric cancer.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yu Wang, Wenyu Sun, Jingfeng Wang, Xu Wang, Yicheng Xu, Yuanzhen Guo, Yeru Wang, Manru Zhang, Long Jiang, Su Liu, Jiadong Huang
Summary: A facile and robust exponential rolling circle amplification (RCA) strategy has been proposed for sensitive biosensing, utilizing primer-remodeling amplification with a repair enzyme and polymerase for detecting UDG activity. This approach shows potential for ultrasensitive detection of low levels of analytes in clinical diagnosis and fundamental biomedicine research.
Article
Nanoscience & Nanotechnology
Min Hou, Xiang Yin, Jianhui Jiang, Jianjun He
Summary: The physically cross-linked DNAzyme-based hydrogel strategy enables specific envelopment and release of targeted cancer cells, allowing for enrichment and subsequent applications. This method offers an attractive approach for isolating viable cancer cells from various cell mixtures for downstream applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Kazi Morshed Alom, Young Jun Seo
Summary: This study developed a triple ligation-based system for simultaneous detection of three microRNAs. The system utilized a multi-ligation-padlock probe, which consisted of three parts complementary to two different microRNAs simultaneously. The probe circularized in the presence of all three microRNAs, allowing for sensitive diagnosis of their presence or absence.
Article
Chemistry, Analytical
Kazi Morshed Alom, Young Jun Seo
Summary: This study developed a triple ligation-based system for detecting three miRNAs simultaneously, demonstrating its selective and sensitive diagnosis of triple miRNAs.
Article
Multidisciplinary Sciences
Gun-Soo Park, Jin-Soo Maeng
Summary: We developed a method for amplifying kilobases-long target sequence from RNA templates using Cre recombinase to generate circular intermediate template for subsequent amplification. The method can amplify a target sequence of about 2.9 kb, and the amplicon can be used for cloning.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Organic
Lingyan Liu, Yan Zhang, Hua Zhang, Kaimeng Huang, Bo-xin Gao, Min Zou, Xin Zhou, Hongkai Wang, Jing Li
ORGANIC & BIOMOLECULAR CHEMISTRY
(2014)
Article
Engineering, Biomedical
Peng Zhang, Xin Zhou, Mei He, Yuqin Shang, Ashley L. Tetlow, Andrew K. Godwin, Yong Zeng
NATURE BIOMEDICAL ENGINEERING
(2019)
Article
Chemistry, Analytical
Xin Zhou, Hongmei Cao, Yong Zeng
Summary: In this study, a pneumatically automated microfluidic reactor device was designed to integrate analyte extraction/enrichment and DSNSA-mediated miRNA detection in a streamlined analysis workflow, achieving rapid and robust on-chip detection of miR-21 with reduced consumption of DSN enzyme. The optimized workflow demonstrated a limit-of-detection of 35 amol and has potential applications in clinical diagnosis and beyond.
Review
Biochemical Research Methods
Shibo Cheng, Yutao Li, He Yan, Yunjie Wen, Xin Zhou, Lee Friedman, Yong Zeng
Summary: Extracellular vesicles, including exosomes, play a significant role in cancer diagnosis and therapeutic monitoring by carrying a selective set of biomolecules from tumor cells. Microfluidics offers a disruptive platform for EV isolation and analysis due to its advantages in promoting the development of new molecular and cellular sensing systems with improved sensitivity and specificity. Despite current challenges in EV research, adapting microfluidic techniques shows promise in assessing emerging EV-associated biomarkers for clinical applications.
Article
Biochemical Research Methods
Xin Zhou, Gopi Chandran Ravichandran, Peng Zhang, Yang Yang, Yong Zeng
Article
Chemistry, Multidisciplinary
Peng Zhang, Xin Zhou, Yong Zeng
Article
Biochemical Research Methods
Peng Zhang, Jennifer Crow, Divya Lella, Xin Zhou, Glenson Samuel, Andrew K. Godwin, Yong Zeng
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)