期刊
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY
卷 29, 期 1, 页码 24-29出版社
SOC BRASILEIRA FARMACOGNOSIA
DOI: 10.1016/j.bjp.2018.11.004
关键词
Astaxanthin; Shrimp shells; HPLC; Extraction optimization; Purification
资金
- National Natural Science Foundation of China [21405162]
- Natural Science Foundation of Shandong Province [ZR2017MEE059]
- Project of Shandong Province Higher Educational Science and Technology Program [J18KA293]
- National Natural Science Foundation Cultivation Project in Jining medical university [JYP201728, JYP201727]
- Teacher Research Support Foundation in Jining medical university [JY2017KJ049]
- Student Innovation Training Program in Jining Medical University [cx2017034, 201710443034]
In this study, the extraction process and purification technology of astaxanthin from shrimp shells were established, and the effects of different treatments on the content of astaxanthin were studied. The determination results of astaxanthin in various shrimp/crab shells showed that the astaxanthin content in the Procambarus clarkia shell reached 239.96 mu g/g. The effects of cool-ventilated, sun-dried and cooking conditions on the content of astaxanthin during the treatment of shrimp shells were investigated respectively and fresh shrimp shells as best extraction source was determined. The nine groups orthogonal test design with four factors and three levels (L-9(3)(4)) was used to analyze the optimization of extraction process of astaxanthin from shrimp shells with ethanol as an environmentally friendly extraction solvent. The optimum experimental condition including the solid-liquid ratio (1:7), extraction time(20 min) and temperature (50 degrees C) was proposed with the maximum extraction yield of astaxanthin. Next, silica gel column chromatography was used to purify the crude extraction of astaxanthin, and the purity of astaxanthin increased from 0.34% to 85.1% (about 250 times), which has great applications in the high value utilization of shrimp shells resources and the development of astaxanthin-related products. (C) 2018 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
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