Article
Cell Biology
Ekene Anakor, Vanessa Milla, Owen Connolly, Cecile Martinat, Pierre Francois Pradat, Julie Dumonceaux, William Duddy, Stephanie Duguez
Summary: Extracellular vesicles play a crucial role in intercellular communication and their study in Amyotrophic Lateral Sclerosis (ALS) is important. The smaller exosome-like vesicles from ALS patients have negative effects on motor neurons, astrocytes, and myotubes, while vesicles from healthy subjects or larger vesicles from ALS or healthy subjects do not exhibit such effects.
Article
Biochemical Research Methods
Ines C. Bras, Mohammad H. Khani, Dietmar Riedel, Iwan Parfentev, Ellen Gerhardt, Christoph van Riesen, Henning Urlaub, Tim Gollisch, Tiago F. Outeiro
Summary: EVs are important mediators in intercellular communication. This study characterizes the proteomes of ectosomes and exosomes, establishes specific protein markers for their distinction, and demonstrates their effects on neuronal activity.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Marco Marchisio, Pasquale Simeone, Giuseppina Bologna, Eva Ercolino, Laura Pierdomenico, Damiana Pieragostino, Alessia Ventrella, Francesca Antonini, Genny Del Zotto, Daniele Vergara, Christian Celia, Luisa Di Marzio, Piero Del Boccio, Antonella Fontana, Domenico Bosco, Sebastiano Miscia, Paola Lanuti
Summary: The study optimized a procedure using PFC to identify and separate circulating EVs from different cell origins, with the use of LCD staining being essential for PFC detection and sorting of EVs from fresh body fluids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Edwina R. Allen, Kaitlyn M. Whitefoot-Keliin, Ellen M. Palmatier, Andrew R. Mahon, Mallary C. Greenlee-Wacker
Summary: This study demonstrates that neutrophils regulate inflammatory response by releasing extracellular vesicles (EVs) and DNA. A23187-EVs induce macrophage inflammation through cGAS-STING-dependent IL-6 production, while EVs from untreated cells do not exhibit pro-inflammatory activity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
Jeremy P. Bost, Osama Saher, Daniel Hagey, Doste R. Mamand, Xiuming Liang, Wenyi Zheng, Giulia Corso, Oskar Gustafsson, Andre Gorgens, Ci Edvard Smith, Rula Zain, Samir El Andaloussi, Dhanu Gupta
Summary: Extracellular vesicles (EVs) are nanosized cell-derived vesicles that facilitate intercellular communication via transmitting biological cargo. The production of EVs is influenced by various pathways and external factors in the cell media, with ceramide-dependent pathway being a major contributor to engineered EV production. The study provides insights into understanding and optimizing EV production in cell culture for developing advanced methods.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Haisheng Liu, Ye Tian, Chengfeng Xue, Qian Niu, Chen Chen, Xiaomei Yan
Summary: This study provides direct and conclusive experimental evidence for an in-depth understanding of how DNA is associated with extracellular vesicles (EVs). The study reveals that EVs carry naked DNA or DNA associated with non-vesicular entities. The quantity and distribution of EV-DNA vary depending on the cell type, with a high heterogeneity observed. The study also shows that external EV-DNA is mainly localized on smaller EVs, while internal EV-DNA is packaged inside larger EVs. The predominant form of EV-DNA is double-stranded DNA, and it is not associated with histone proteins.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Biochemistry & Molecular Biology
Magdalena E. Marzec, Carina Rzaca, Pawel Moskal, Ewa L. Stepien
Summary: Time-of-flight secondary ion mass spectrometry (TOF-SIMS) with the Bi3thorn liquid metal ion gun was used to investigate the content of lipids and amino acids in extracellular vesicles. The study confirmed the detrimental effect of hyperglycemia on lipids and amino acids in released extracellular vesicles.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Orthopedics
Tengjing Xu, Yunting Lin, Xinning Yu, Guangyao Jiang, Jiajie Wang, Kaiwang Xu, Jinghua Fang, Siheng Wang, Xuesong Dai
Summary: This study compared the effects of adipose stem cell-derived exosomes and ectosomes on Achilles tendinopathy. The results showed that ASC-Exos had more beneficial effects on tendon repair than ASC-Ectos.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Review
Physiology
Derek W. Russell, Kristopher R. Genschmer, J. Edwin Blalock
Summary: Chronic obstructive pulmonary disease (COPD) is a complex smoking-related disease driven by interrelated processes. Extracellular vesicles (EVs) are powerful effectors that could play a significant role in COPD pathogenesis.
ANNUAL REVIEW OF PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Naia Risager Wright, Mathew M. Jessop-Fabre, Benjamin J. Sanchez, Tune Wulff, Christopher T. Workman, Nanna Petersen Ronnest, Nikolaus Sonnenschein
Summary: In this study, we investigated the adaptation of a recombinant Saccharomyces cerevisiae strain at the single-cell level under glucose-limited conditions. We identified three apparent phenotypes underlying the adaptive response: cells with reduced insulin production, cells with reduced enzymatic capacity in central carbon metabolism, and cells exhibiting pseudohyphal growth. These findings highlight the importance of considering population heterogeneity in studying adaptation and evolution.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Hematology
Aaron Scott, Lorena Sueiro Ballesteros, Marston Bradshaw, Ann Power, James Lorriman, John Love, Danielle Paul, Andrew Herman, Costanza Emanueli, Rebecca J. Richardson
Summary: We have established a transgenic zebrafish model for in vivo identification, tracking, and extraction of endogenous EVs produced by different cell types. Using a membrane-tethered fluorophore reporter system, we demonstrated that multiple cell types actively produce EVs in zebrafish. High spatiotemporal resolution light-sheet live imaging and modified flow cytometry methods allow for tracking and evaluation of cell-type specific EVs, while cryo electron microscopy reveals the full morphological diversity of larval and adult EVs. This zebrafish model can be utilized for the investigation of endogenous EVs in cardiovascular biology and pathology.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Xiaoyi Gao, Xucong Teng, Yicong Dai, Jinghong Li
Summary: The study developed a sensitive RCA-assisted FCA method for detecting exosomes and quantifying their surface proteins, showing excellent detection limits. By changing the protein recognition sequence of the DNA primer, variable compositions of four protein markers on exosomes from different cell sources were sensitively detected.
Article
Multidisciplinary Sciences
Anna Pascual-Reguant, Ralf Koehler, Ronja Mothes, Sandy Bauherr, Daniela C. Hernandez, Ralf Uecker, Karolin Holzwarth, Katja Kotsch, Maximilian Seidl, Lars Philipsen, Werner Mueller, Chiara Romagnani, Raluca Niesner, Anja E. Hauser
Summary: ILCs are essential regulators of biological processes. The study combines multiplexed imaging and computational pipelines to identify tonsillar IRF4 (+) ILC3s and conserved stromal landmarks for ILC localization, offering a platform for future ILC research.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Swarnagowri Vaidyanathan, Harshani Wijerathne, Sachindra S. T. Gamage, Farhad Shiri, Zheng Zhao, Junseo Choi, Sunggook Park, Malgorzata A. Witek, Collin McKinney, Matthew Verber, Adam R. Hall, Katie Childers, Taryn McNickle, Shalee Mog, Elaine Yeh, Andrew K. Godwin, Steven A. Soper
Summary: We have developed a chip-based extended nano-Coulter counter (XnCC) capable of detecting nanoparticles affinity-selected from biological samples with an extremely low concentration limit-of-detection. The XnCC contains 5 in-plane pores and can efficiently detect single particles translocating through these pores. The XnCC has been successfully utilized for counting affinity-selected SARS-CoV-2 viral particles and ovarian cancer extracellular vesicles, demonstrating its potential in clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Editorial Material
Cell Biology
Kenneth W. Witwer, Deborah C. Goberdhan, Lorraine O'Driscoll, Clotilde Thery, Joshua A. Welsh, Cherie Blenkiron, Edit Buzas, Dolores Di Vizio, Uta Erdbrugger, Juan M. Falcon-Perez, Qing-Ling Fu, Andrew F. Hill, Metka Lenassi, Jan Lotvall, Rienk Nieuwland, Takahiro Ochiya, Sophie Rome, Susmita Sahoo, Lei Zheng
Summary: The MISEV initiative by ISEV aims to advance EV science globally, with the 2018 release of MISEV2018 providing comprehensive recommendations on EV nomenclature, sample processing, separation concentration, characterization, functional studies, and reporting requirements, with input from over 400 scientists. ISEV sought feedback and opinions from the community through a survey to better formulate MISEV and address key issues.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Multidisciplinary Sciences
Katja Luck, Dae-Kyum Kim, Luke Lambourne, Kerstin Spirohn, Bridget E. Begg, Wenting Bian, Ruth Brignall, Tiziana Cafarelli, Francisco J. Campos-Laborie, Benoit Charloteaux, Dongsic Choi, Atina G. Cote, Meaghan Daley, Steven Deimling, Alice Desbuleux, Amelie Dricot, Marinella Gebbia, Madeleine F. Hardy, Nishka Kishore, Jennifer J. Knapp, Istvan A. Kovacs, Irma Lemmens, Miles W. Mee, Joseph C. Mellor, Carl Pollis, Carles Pons, Aaron D. Richardson, Sadie Schlabach, Bridget Teeking, Anupama Yadav, Mariana Babor, Dawit Balcha, Omer Basha, Christian Bowman-Colin, Suet-Feung Chin, Soon Gang Choi, Claudia Colabella, Georges Coppin, Cassandra D'Amata, David De Ridder, Steffi De Rouck, Miquel Duran-Frigola, Hanane Ennajdaoui, Florian Goebels, Liana Goehring, Anjali Gopal, Ghazal Haddad, Elodie Hatchi, Mohamed Helmy, Yves Jacob, Yoseph Kassa, Serena Landini, Roujia Li, Natascha van Lieshout, Andrew MacWilliams, Dylan Markey, Joseph N. Paulson, Sudharshan Rangarajan, John Rasla, Ashyad Rayhan, Thomas Rolland, Adriana San-Miguel, Yun Shen, Dayag Sheykhkarimli, Gloria M. Sheynkman, Eyal Simonovsky, Murat Tasan, Alexander Tejeda, Vincent Tropepe, Jean-Claude Twizere, Yang Wang, Robert J. Weatheritt, Jochen Weile, Yu Xia, Xinping Yang, Esti Yeger-Lotem, Quan Zhong, Patrick Aloy, Gary D. Bader, Javier De las Rivas, Suzanne Gaudet, Tong Hao, Janusz Rak, Jan Tavernier, David E. Hill, Marc Vidal, Frederick P. Roth, Michael A. Calderwood
Article
Multidisciplinary Sciences
Shilpa Chennakrishnaiah, Thupten Tsering, Caroline Gregory, Nadim Tawil, Cristiana Spinelli, Laura Montermini, Nicolaos Karatzas, Saro Aprikian, Dongsic Choi, Ludger Klewes, Sabine Mai, Janusz Rak
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Multidisciplinary
Elisa Lazaro-Ibanez, Farid N. Faruqu, Amer F. Saleh, Andreia M. Silva, Julie Tzu-Wen Wang, Janusz Rak, Khuloud T. Al-Jamal, Niek Dekker
Summary: The study evaluated the effects of five different tracers on the in vivo biodistribution of extracellular vesicles, finding that indium and DiR were the most sensitive tracers. Genetic modification of EVs may impact their physiological distribution in vivo.
Article
Cell & Tissue Engineering
Anastasia Cheng, Dongsic Choi, Maximilien Lora, Dominique Shum-Tim, Janusz Rak, Ines Colmegna
STEM CELL RESEARCH & THERAPY
(2020)
Editorial Material
Hematology
Janusz Rak
Summary: Researchers have long been puzzled by the abnormal clotting in cancer patients, while CAT continues to have serious consequences. Dunbar et al. in Blood embark on a bold attempt to seek possible answers.
Article
Hematology
Nadim Tawil, Rayhaan Bassawon, Brian Meehan, Ali Nehme, Laura Montermini, Tenzin Gayden, Nicolas De Jay, Cristiana Spinelli, Shilpa Chennakrishnaiah, Dongsic Choi, Lata Adnani, Michele Zeinieh, Nada Jabado, Claudia L. Kleinman, Michael Witcher, Yasser Riazalhosseini, Nigel S. Key, David Schiff, Steven P. Grover, Nigel Mackman, Charles P. Couturier, Kevin Petrecca, Mario L. Suva, Anoop Patel, Itay Tirosh, Hamed Najafabadi, Janusz Rak
Summary: This study found that PDPN and TF in GBM activate platelets and the clotting cascade through extracellular vesicles, promoting thrombosis, which could be potential targets for treatment.
Article
Chemistry, Multidisciplinary
Imman I. Hosseini, Zezhou Liu, Xavier Capaldi, Tamer AbdelFatah, Laura Montermini, Janusz Rak, Walter Reisner, Sara Mahshid
Summary: Extracellular vesicles (EVs) are cell-derived membrane structures circulating in body fluids, showing potential for noninvasive diagnosis. To fully utilize the diagnostic potential of EVs, technologies that can analyze large populations of EVs without sacrificing single EV detail are needed.
Article
Cell Biology
Dongsic Choi, Laura Montermini, Brian Meehan, Anthoula Lazaris, Peter Metrakos, Janusz Rak
Summary: Overexpression of mutant RAS drives a switch in the internalization of extracellular vesicles (EVs) from endocytosis to macropinocytosis in cancer cells, enhancing EV uptake. This process depends on surface proteoglycan, fibronectin, and the EV engulfment mechanism regulated by CRAF.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Anita K. Luu, Mia Cadieux, Mackenzie Wong, Rachel Macdonald, Robert Jones, Dongsic Choi, Michelle Oblak, Brigitte Brisson, Scott Sauer, James Chafitz, David Warshawsky, Geoffrey A. Wood, Alicia M. Viloria-Petit
Summary: Osteosarcoma (OS) is a highly malignant bone tumor with limited improvements in treatment. Understanding the molecules contributing to OS biology can lead to the discovery of novel therapies. This study demonstrates the potential of extracellular vesicles (EVs) in revealing protein signatures related to OS and highlights a specific protein, PSMD14/Rpn11, as a potential prognostic marker and therapeutic target for OS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Lata Adnani, Jordan Kassouf, Brian Meehan, Cristiana Spinelli, Nadim Tawil, Ichiro Nakano, Janusz Rak
Summary: The study reveals that extracellular vesicles from endothelial cells can promote the transition of GSCs from proneural to mesenchymal by activating MMPs and NFkB, and inactivating NOTCH.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Janusz Rak, Leon Strzadala
Summary: Extracellular vesicles and particles are unique carriers of molecular information that play increasingly recognized roles in various aspects of health and disease. In cancer, the complex cellular interactions and systemic responses can be reflected in the molecular composition of the EVs/EPs released into the bloodstream. Current liquid biopsy approaches focus on specific elements of EV/EP cargo as potential biomarkers, but emerging technologies enable a more holistic analysis at the single particle level. Longitudinal molecular mapping of EV/EP populations may provide a new dimension for understanding cancer biology, personalized diagnostics, and therapy.
ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS
(2023)
Article
Cell Biology
Solchan Won, Changjin Lee, Seoyoon Bae, Jaemin Lee, Dongsic Choi, Min-Gang Kim, Sunghyun Song, Jaewook Lee, Eunhye Kim, HaYoung Shin, Anita Basukala, Tae Ryong Lee, Dong-Sup Lee, Yong Song Gho
Summary: Despite the challenges in large-scale production, Escherichia coli outer membrane vesicles (OMVs) have potent anti-tumour activities and show synergy with anti-PD-1 immunotherapy. Mass-produced E. coli OMVs led to complete remission in mouse tumour models and increased infiltration and activation of cancer antigen-specific CD8(+) T cells in the tumour microenvironment. These findings highlight the potential of mass-produced E. coli OMVs as next-generation cancer immunotherapeutic agents.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Correction
Oncology
Arik Drucker, Byong Hoon Yoo, Iman Aftab Khan, Dongsic Choi, Laura Montermini, Xiaoyang Liu, Sanja Jovanovic, Tallal Younis, Kirill V. Rosen
BREAST CANCER RESEARCH
(2020)
Article
Oncology
Arik Durcker, Byong Hoon Yoo, Iman Aftab Khan, Dongsic Choi, Laura Montermini, Xiaoyang Liu, Sanja Jovanovic, Tallal Younis, Kirill V. Rosen
BREAST CANCER RESEARCH
(2020)
Article
Cell Biology
Dongsic Choi, Gyeongyun Go, Dae-Kyum Kim, Jaewook Lee, Seon-Min Park, Dolores Di Vizio, Yong Song Gho
JOURNAL OF EXTRACELLULAR VESICLES
(2020)