期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 116, 期 6, 页码 2318-2327出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1811768116
关键词
RNA editing; Drosophila; neurons
资金
- Israel Science Foundation [384/14]
- United States Israel Binational Science Foundation [2015275]
- National Institutes of Health (NIH) [R01 GM102484, R01 GM124215, R01 MH115080]
- National Science Foundation Graduate Research Fellowship [DGE-114747]
- NIH Training Grant [NIH-NIGMS T32 GM007790]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [2015275] Funding Source: National Science Foundation
Adenosine-to-inosine (A-to-I) RNA editing, catalyzed by ADAR enzymes, is a ubiquitous mechanism that generates transcriptomic diversity. This process is particularly important for proper neuronal function; however, little is known about how RNA editing is dynamically regulated between the many functionally distinct neuronal populations of the brain. Here, we present a spatial RNA editing map in the Drosophila brain and show that different neuronal populations possess distinct RNA editing signatures. After purifying and sequencing RNA from genetically marked groups of neuronal nuclei, we identified a large number of editing sites and compared editing levels in hundreds of transcripts across nine functionally different neuronal populations. We found distinct editing repertoires for each population, including sites in repeat regions of the transcriptome and differential editing in highly conserved and likely functional regions of transcripts that encode essential neuronal genes. These changes are site-specific and not driven by changes in Adar expression, suggesting a complex, targeted regulation of editing levels in key transcripts. This fine-tuning of the transcriptome between different neurons by RNA editing may account for functional differences between distinct populations in the brain.
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