The development of antioxidant system in the intestinal tract of broiler chickens

The gastrointestinal tract is the site for the uptake of nutrients from the external environment. We hypothesized that the antioxidant system in the intestinal tract has a vital protective role from the oxidative damage induced by oxidants in foods. The aim of this study was to investigate the development of the antioxidant system in the intestine of chickens. The activity and gene expression of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and the content of the non-enzymatic substance glutathione (GSH) were measured in the duodenum, jejunum, and ileum of chickens at 1, 3, 7, 11, 14, 21, 35, and 42 d of age. The results showed that the small intestinal tract had relatively higher SOD activity and GSH concentration and lower CAT and GSH-Px activities, compared with those of other visceral organs. CAT and GSH-Px activities and GSH concentration showed a decreasing trend with age, whereas SOD activity was not significantly influenced by age. The gene expression of SOD1, SOD2, and GSH-Px7 showed a dramatic decrease from 3 d of age. The results indicated that SOD and GSH were highly expressed in the first week of age after hatching. To conclude, the results suggest that SOD and GSH play a vital protective role in the small intestine after hatching, which contributes to rapid development of the intestine.