Article
Plant Sciences
Felix Moran, Mario Herrero-Cervera, Sofia Carvajal-Rojas, Ester Marco-Noales
Summary: Huanglongbing (HLB) is a devastating disease that affects all commercial citrus species worldwide, and it is transmitted by psyllid vectors. This study presents a new real-time recombinase polymerase amplification (RPA) protocol that can detect the three bacteria species associated with HLB in both plant and insect samples. The protocol is accurate, sensitive, specific, and reliable, and it does not require specialized equipment, making it ideal for field use. The results indicate the potential of this new RPA protocol to be implemented as a reliable on-site detection kit for HLB.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Rachel J. Rattner, Kris E. Godfrey, Subhas Hajeri, Raymond K. Yokomi
Summary: In this study, a recombinase polymerase amplification (RPA) assay was developed for rapid and portable detection of Huanglongbing (HLB), a destructive citrus disease. The assay, which does not require DNA purification, allows for real-time detection within 8 minutes. The sensitivity and specificity of the RPA assay were comparable to the standard method, quantitative polymerase chain reaction (qPCR). The assay can be used in the field and accurately distinguish similar disease symptoms caused by different pathogens, aiding in the control of HLB spread.
Review
Microbiology
Chuanyu Yang, Veronica Ancona
Summary: This review summarizes the main aspects of mechanisms against citrus greening, including inhibition of virulence genes, activation of plant defense genes and metabolites, and the role of citrus microbiome in combatting the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Subhas Hajeri, Sandra Olkowski, Lucita Kumagai, Neil Mcroberts, Raymond K. Yokomi
Summary: Early detection and prompt response are crucial in eradicating 'huanglongbing' in California. A study found that testing the peduncle tissue of fruit consistently resulted in higher detection rates of the disease compared to other tissue types.
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Bernard Reynaud, Patrick Turpin, Florencia M. Molinari, Martial Grondin, Solene Roque, Frederic Chiroleu, Alberto Fereres, Helene Delatte
Summary: Huanglongbing (HLB) is a serious disease of citrus worldwide, caused by three non-cultivable bacteria. The African psyllid Trioza erytreae is capable of efficiently acquiring and transmitting the bacteria, highlighting the high risk of spread of the disease in Europe.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Haoqi Wang, Nirmitee Mulgaonkar, Samavath Mallawarachchi, Manikandan Ramasamy, Carmen S. Padilla, Sonia Irigoyen, Gitta Coaker, Kranthi K. Mandadi, Sandun Fernando
Summary: This study screened antimicrobial peptides that have the potential to inhibit the growth of citrus greening bacteria. The findings were further validated by in vitro experiments and the binding mechanism was analyzed. These findings are important for designing effective therapies to manage plant diseases associated with citrus greening.
Article
Plant Sciences
Monica N. Alves, Laudecir L. Raiol-Junior, Eduardo A. Girardi, Maeva Miranda, Nelson A. Wulff, Everton Carvalho, Silvio A. Lopes, Jesus A. Ferro, Patrick Ollitrault, Leandro Pena
Summary: Huanglongbing (HLB), the most destructive citrus disease, is associated with the Candidatus Liberibacter species Ca. L. asiaticus (Las). In this study, it was found that certain Oceanian citrus genotypes and hybrids showed full resistance to Las, with their leaves remaining Las-free even when the susceptible rootstock was infected. However, Las was detected in the stem bark above the graft union. Further experiments confirmed that the insects feeding on the Oceanian citrus plants and their canopies, new flushes, and leaves from rooted cuttings remained negative for Las, and their budwood pieces were unable to infect sweet orange through grafting.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Minli Bao, Zheng Zheng, Jianchi Chen, Xiaoling Deng
Summary: Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide, and in China it is associated with the unculturable alpha-proteobacterium Candidatus Liberibacter asiaticus (CLas). Phages/prophages of CLas have been identified through genomic research, aiding in understanding CLas biology. This study investigated the symptom development of different citrus cultivars infected with CLas strains carrying Type 1 and Type 2 phages, providing baseline information for future research on the roles of CLas phages in HLB symptom development.
Article
Plant Sciences
Monica N. Alves, Silvio A. Lopes, Laudecir L. Raiol-Junior, Nelson A. Wulff, Eduardo A. Girardi, Patrick Ollitrault, Leandro Pena
Summary: Huanglongbing (HLB) is the most destructive and incurable disease of citrus, with no resistant citrus genotypes currently available. Some citrus relatives have been identified as fully resistant to HLB, but they are phylogenetically distant from citrus. By evaluating a diverse collection of graft-compatible Citrinae species, new potential sources of resistance to HLB were identified, including Eremocitrus glauca and Papua/New Guinea Microcitrus species.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Josiane Cecilia Darolt, Flavia de Moura Manoel Bento, Bruna Lais Merlin, Leandro Pena, Fernando Luis Consoli, Nelson Arno Wulff
Summary: The study investigated the molecular interactions of Candidatus Liberibacter asiaticus (Las) with the Asian citrus psyllid, Diaphorina citri, during the vector acquisition process. Results demonstrated active metabolism and increased gene expression in Las genome within the psyllid gut, with genes involved in energy production, amino acid metabolism, and other functions being highly expressed. The research provides insight into the initial steps of Las gut colonization in D. citri, showing that 95% of Las genes were active during this process.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Pan Shen, Xueyi Li, Shimin Fu, Changyong Zhou, Xuefeng Wang
Summary: This study found that a peptide called SECP8 secreted by the citrus huanglongbing bacterium CLas has the ability to suppress immune responses and promote disease development. It also affects the expression of immune-related genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Fanglan Gao, Bo Wu, Chengwu Zou, Yixue Bao, Dean Li, Wei Yao, Charles A. Powell, Muqing Zhang
Summary: Huanglongbing, caused by Candidatus Liberibacter asiaticus, is the most damaging citrus disease globally and has spread across regions in China. The disease affects oranges the most severely and pomelos the least. CLas bacteria associated with specific geographical and citrus variety infect over 50% of symptomatic samples, and variations in hypervariable genomic regions play a key role in the evolution and adaptation of the bacteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Rui-Xu Jiang, Feng Shang, Hong-Bo Jiang, Wei Dou, Tomislav Cernava, Jin-Jun Wang
Summary: This study investigates the impact of the Candidatus Liberibacter asiaticus (CLas) pathogenic bacteria on the bacterial community in different color morphs, genders, and tissues of the Asian citrus psyllid (ACP). The results show that there are no significant differences in bacterial community diversity and CLas acquired ratio between different color morphs and genders of ACP adults. However, acquiring CLas promotes the diversity and richness of the adult bacterial community. CLas also changes the bacterial structure in different tissues, making the bacterial relationship network simpler than in an uninfected condition. These findings provide essential knowledge on ACP symbionts and the effects of CLas on the ACP bacterial community.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Jiaquan Huang, Iobana Alanis-Martinez, Lucita Kumagai, Zehan Dai, Zheng Zheng, Adalberto A. Perez de Leon, Jianchi Chen, Xiaoling Deng
Summary: This study showed that there were at least two different introductions of CLas into Mexico, and the CLas strains between Mexico and the USA are closely related. Two Geno-groups were established, which provide the basis for future CLas subspecies research.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dilip Kumar Ghosh, Amol D. Kokane, Sunil B. Kokane, Jigme Tenzin, Mrugendra G. Gubyad, Phuntsho Wangdi, Ashutosh A. Murkute, Ashwani Kumar Sharma, Siddarame Gowda
Summary: Citrus, especially mandarin, is an economically important fruit crop in Bhutan, but faces challenges of early decline and low productivity due to the presence of Candidatus Liberibacter asiaticus and citrus tristeza virus (CTV). Molecular analysis revealed a high incidence of these pathogens in symptomatic citrus trees, providing valuable insights into their association with citrus decline in Bhutan.
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Poonam Dhankhar, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: The study utilized structure-based drug design approach to screen and identify potent molecules against 2Cys Prx, which showed higher binding affinity with Prx compared to its substrate. Molecular dynamics analysis revealed that the Prx-inhibitor complexes were more stable.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biotechnology & Applied Microbiology
Sunil B. Kokane, Pragati Misra, Amol D. Kokane, Mrugendra G. Gubyad, Ashish J. Warghane, Datta Surwase, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: Tristeza is an economically important disease of citrus caused by Citrus tristeza virus, which has caused significant losses to the industry worldwide. Studies on virus distribution and tropism in citrus plants are necessary to understand the reason behind this scenario.
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Ruchi Rani, Amol D. Kokane, Dilip Kumar Ghosh, Shailly Tomar, Ashwani Kumar Sharma
Summary: In this study, the recombinant cytoplasmic 2-Cys peroxiredoxin from Citrus sinensis (CsPrx) was purified and characterized. The reduced state of the protein had higher alpha-helical content. CsPrx was found to provide protection against ROS and free radicals, and it also promoted cell proliferation and wound healing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Microbiology
Dilip Kumar Ghosh, Amol Kokane, Sunil Kokane, Krishanu Mukherjee, Jigme Tenzin, Datta Surwase, Dhanshree Deshmukh, Mrugendra Gubyad, Kajal Kumar Biswas
Summary: This study detected a high incidence of citrus tristeza virus (CTV) infection in Mandarin orange samples from Bhutan using reverse transcription PCR (RT-PCR). Genetic variability among Bhutanese CTV variants was analyzed by sequencing four independent genomic regions and comparing with GenBank deposited sequences. The study identified unexpected diversity among the Bhutanese CTV variants and also discovered a resistant-breaking variant. The findings contribute to the understanding of CTV diversity and provide insights into pathogenicity assessments.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Deena Nath Gupta, Vikram Dalal, Brajesh Kumar Savita, Md Shahid Alam, Anamika Singh, Mrugendra Gubyad, Dilip Kumar Ghosh, Pravindra Kumar, Ashwani Kumar Sharma
Summary: In this study, the Bacterioferritin comigratory protein family 1 Cys peroxiredoxin from Candidatus Liberibacter asiaticus (CLaBCP) was biochemically studied and screened for potential antibacterial molecules. The results showed that CLaBCP has enzymatic activity and undergoes structural changes at different pH and temperature ranges. A structure-based drug design method was used to identify more efficient molecules, and molecular dynamics studies confirmed their stability.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Plant Sciences
Dilip Ghosh, Sunil Kokane, Brajesh Kumar Savita, Pranav Kumar, Ashwani Kumar Sharma, Ali Ozcan, Amol Kokane, Swadeshmukul Santra
Summary: Huanglongbing (HLB), also known as citrus greening, is a devastating disease of citrus trees caused by Candidatus Liberibacter asiaticus (CLas) bacteria, which are transmitted by psyllid vectors. Current control measures for HLB are ineffective in the long term, leading to difficulty in preventing disease spread. To combat HLB, a multipronged management strategy is necessary, targeting the pathogen, host, and vector.
Article
Biotechnology & Applied Microbiology
Mustafa Vohra, Manjula Babariya, Jitendrakumar S. Parmar, Narayan Kamath, Ashish Warghane, Dolatsinh Zala
Summary: The emergence of antimicrobial resistance and virulence in clinical isolates is a significant public health concern. This study demonstrated the importance of integrating multiple detection methodologies to accurately detect these traits. The results showed a high prevalence of antimicrobial resistance and virulence factors in isolates from tertiary clinical hospitals. The integration of multiple detection methodologies has the potential to improve detection levels, leading to better patient outcomes and reduced spread of these essential traits.
Article
Oncology
Ashish Warghane, Tejaswini Petkar, Usha S. Preeyaa, Nishi Kumari, Lavanya Ranjan
Summary: The study conducted comparative genomic analysis on the spike protein gene sequences of the novel coronavirus, identifying multiple variations in nucleotide and amino acid positions. Phylogenetic analysis revealed patterns of virus spread across affected countries, with some strains showing community transmission while majority exhibited a mosaic pattern.
EURASIAN JOURNAL OF MEDICINE AND ONCOLOGY
(2021)
