Article
Agronomy
Wei Xin, Lina Zhang, Jiping Gao, Wenzhong Zhang, Jun Yi, Xiaoxi Zhen, Congyuan Bi, Dawei He, Shiming Liu, Xinyu Zhao
Summary: This study investigated the changes in rice root traits under low, normal, and high nitrogen conditions through root morphology analysis. Results indicated that root growth was promoted under low nitrogen conditions and inhibited under high nitrogen conditions. Differential abundance of proteins involved in various pathways was identified as an important reason for changes in root morphology.
Article
Agriculture, Multidisciplinary
Yan Wang, Xunmin Cheng, Tianyuan Yang, Yanlei Su, Shijia Lin, Shupei Zhang, Zhaoliang Zhang
Summary: Theanine and flavonoids are the major secondary metabolites synthesized in tea plant roots, with nitrogen playing a crucial role in their biosynthesis. This study identified proteins responsive to nitrogen deficiency and found that nitrogen promotes theanine synthesis while repressing flavonoid biosynthesis. The ubiquitylome analysis revealed that key enzymes in theanine and flavonoid biosynthesis are modified by ubiquitination, providing insights into the post-translational regulation of secondary metabolism in tea plants.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agronomy
Xinxin Li, Xinghua Zhang, Qingsong Zhao, Hong Liao
Summary: Acid soils cover approximately 50% of arable lands, and improving crop productivity in these soils is crucial for food security and agricultural sustainability. The combination of high soil acidity, phosphorus deficiency, and aluminum toxicity severely affects crop growth and yield. Legumes, such as soybeans, have evolved mechanisms to adapt to these stresses, including root architecture modification, exudation, and symbiosis. Breeding legume cultivars with superior nutrient efficiency and aluminum tolerance traits through genetic selection could be a powerful strategy for maintaining or improving yields in challenging soil conditions.
Article
Plant Sciences
Yin-Hua Lai, Ming-Yi Peng, Rong-Yu Rao, Wen-Shu Chen, Wei-Tao Huang, Xin Ye, Lin-Tong Yang, Li-Song Chen
Summary: Extensive metabolic and gene reprogramming occurred in Citrus sinensis seedlings under nitrogen deficiency, enhancing the ability to maintain phosphate and nitrogen homeostasis, improving energy production and utilization, enhancing metabolite transport, and activating protein processing. Additionally, the roots under nitrogen deficiency exhibited higher antioxidant capacity and delayed senescence.
Article
Plant Sciences
Jia Hao, Qiang Li, Hongjun Yu, Heng Wang, Lin Chai, Tiantian Miao, Weijie Jiang
Summary: Nitrogen deficiency has a significant impact on the metabolic pathways of cucumber fruit, especially in carbon metabolism, amino acid biosynthesis, and ascorbate metabolism. The research results indicate that under nitrogen deficiency, protein expression in cucumber fruit is altered.
HORTICULTURAL PLANT JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Bing Liu, Jianxiang Fang, Huizhen Chen, Yuehong Sun, Shan Yang, Qian Gao, Ying Zhang, Chang Chen
Summary: This study characterized GcvB in V. alginolyticus and determined its regulon through transcriptome and quantitative proteome analysis. The results suggest that GcvB plays a critical role in amino acid metabolism and virulence regulation in V. alginolyticus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Zheng-He Lin, Chang-Song Chen, Qiu-Sheng Zhong, Qi-Chun Ruan, Zhi-Hui Chen, Xiao-Mei You, Rui-Yang Shan, Xin-Lei Li
Summary: This study highlights the different effects of N deficiency on the metabolism of tea leaves and roots. N-deficient tea leaves showed chlorosis, reduced biomass accumulation, and altered metabolite concentrations, while N-deficient tea roots exhibited increased accumulation of certain substances. N deficiency may lead to lower tea quality in leaves, but improved stress response in roots.
Article
Biochemistry & Molecular Biology
Yiman Li, Wendong Zhang, Dalong Zhang, Yinjian Zheng, Yaliang Xu, Binbin Liu, Qingming Li
Summary: This study used proteomic analysis to investigate the effects of carbon dioxide concentration enrichment on cucumber seedling root under drought stress. The results showed that carbon dioxide enrichment promoted energy and amino acid metabolism, as well as cell wall regulation ability. However, the effectiveness of carbon dioxide enrichment in alleviating drought stress was limited under severe conditions, possibly due to excessive damage to the seedlings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jie Jin, Jianfeng Wang, Keke Li, Shengwang Wang, Juan Qin, Guohong Zhang, Xiaofan Na, Xiaomin Wang, Yurong Bi
Summary: This study analyzed salt tolerance mechanisms in different soybean cultivars through physiological, transcriptomic, metabolomic, and bioinformatic methods. Salt-tolerant cultivars showed better root growth, higher antioxidant enzyme activities, lower ion accumulation, and differential gene expression and metabolic pathways under salt stress, which contributed to their improved adaptation to salinity stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mingjie Zhou, Wenzhu Zhao, Wenjun Xue, Jingbo Liu, Zhipeng Yu
Summary: This study clarified the antihypertensive mechanisms of QIGLF on spontaneously hypertensive rats by using a serum proteomic approach. QIGLF was found to potentially exert antihypertensive effects by inhibiting Na+ reabsorption and oxidative stress, and restoring gap junction and tight junction.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Genetics & Heredity
Yafang Wang, Pan Zhang, Le Li, Danning Li, Zheng Liang, Yuman Cao, Tianming Hu, Peizhi Yang
Summary: In this study, we found that rhizobium symbiosis in alfalfa enhances salt tolerance by regulating multiple physiological processes, including stress response, antioxidant ability, and ion homeostasis.
Article
Plant Sciences
Jialiang Li, Rui Yang, Yonglei Jiang, Shubin Sun, Junying Li, Hao Gu, Ying Lin, Xianxue Luo, Chenggang He, Yi Chen
Summary: This study provides insights into the responses of tobacco roots to different soil types, showing that red soil promotes the coordination of chemical composition of tobacco leaves and the growth and activity of the root system, while limestone soil and paddy soil have relatively weaker effects.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Zhenhua Zhang, Zhimin Wu
Summary: Increasing atmospheric CO2 concentrations impact the carbon-nitrogen balance in crops, altering fertilizer use efficiency. In this study, Brassica napus was cultivated under different CO2 and NO3 -N concentrations to examine the influence of C/N ratio on plant growth. The findings showed that elevated CO2 enhanced biomass and nitrogen assimilation efficiency under low NO3 -N conditions, indicating an adaptation by Brassica napus. Transcriptome and metabolome analyses revealed that elevated CO2 promoted amino acid catabolism under low NO3 -N conditions. This study provides new insights into Brassica napus adaptation to environmental change.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
Zhuorong Li, Shurong Deng, Dongyue Zhu, Jiangting Wu, Jing Zhou, Wenguang Shi, Payam Fayyaz, Zhi-Bin Luo, Jie Luo
Summary: Using physiological and proteomic assays, we found that N deficiency inhibits the growth, and N uptake and assimilation in poplar roots, as well as the levels of phytohormones such as abscisic acid and salicylic acid. On the other hand, high N stimulates these processes and leads to differentially abundant proteins in poplar roots. Our results suggest a correlation between proteomic reconfigurations and physiological alterations in poplars acclimating to changing N availability.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Yingning Wang, Fang Ma, Jixian Yang, Haijuan Guo, Delin Su, Lan Yu
Summary: The study revealed the genetic and transcriptional strategies used by Xanthobacter sp. YN2 during dioxane degradation, including multiple metabolic pathways and gene regulations. The strain rapidly increased biomass, achieved high degradation efficiency and quickly adapted to dioxane exposure during the degradation process, providing useful information regarding the molecular basis for efficient dioxane biodegradation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Cora M. Best, Robert Sherwood, Janet A. Novotny, Sheng Zhang, Eva K. Pressman, Kimberly O. O'Brien
Summary: The study aimed to investigate the kinetics of vitamin D in pregnant and non-pregnant women using stable isotopes. The results showed that pregnant women had a longer half-life of 25(OH)D-3 and a positive correlation between AUC of d3-25(OH)D-3 and serum DBP concentration.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leiyun Yang, Zhixue Wang, Aiqin Zhang, Ruchika Bhawal, Chunlong Li, Sheng Zhang, Lailiang Cheng, Jian Hua
Summary: Disruption of the LOS2 gene leads to increased autoimmunity in plants, partially dependent on NLR genes. Autoimmunity in los2 mutants affects the accumulation of sugars and organic acids, as well as the depletion of glycolytic metabolites.
Article
Environmental Sciences
Yuqi Wang, Yan Kang, Wancong Yu, Sangbom M. Lyi, Hyong Woo Choi, Enzong Xiao, Li Li, Daniel F. Klessig, Jiping Liu
Summary: This study found that the tonoplast intrinsic protein AtTIP2;2 in Arabidopsis is involved in zinc immobilization and resistance. It helps retain excess zinc in the root, limits its translocation, and facilitates accumulation in leaf trichomes. The findings provide new insights into the role of aquaporins in heavy metal resistance and detoxification in plants.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biochemical Research Methods
Jesse Potts, Hui Li, YaoGuo Qin, Xingbo Wu, Dafeng Hui, Kamal Al Nasr, Suping Zhou, Yang Yong, Tara Fish, Jiping Liu, Theodore W. Thannhauser
Summary: This study used laser capture microdissection (LCM) to collect samples from different cell layers of tomato root-tips and investigated the mechanisms related to aluminum toxicity through proteomic analysis. The study found differences in protein expression between the outer-layer cells and the inner tissues, and proteins related to aluminum tolerance were highly accumulated in the outer-layer cells. Additionally, the proteomes of the treated outer-layer cells were different from the untreated counterpart cells.
JOURNAL OF PROTEOMICS
(2022)
Article
Plant Sciences
Wancong Yu, Zhaohui Xue, Xianzheng Zhao, Rui Zhang, Jiping Liu, Sandui Guo
Summary: The transcription of GhAG2 was significantly enhanced by glyphosate treatment, and overexpression of GhAG2 in transgenic plants improved salt and salicylic acid stress tolerance. The study identified differentially expressed genes in response to glyphosate treatment and highlighted the potential of GhAG2 in enhancing stress tolerance in crops. The results provide insights into glyphosate-induced transcriptional changes, potential applications of GhAG2 in crop improvement, and the development of a glyphosate-inducible gene expression system in plants.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Arnon Afonso de Souza Cardoso, Fabricio Teixeira de Lima Gomes, Joao Renato Rodrigues Antonio, Luiz Roberto Guimaraes Guilherme, Jiping Liu, Li Li, Maria Ligia de Souza Silva
Summary: The interaction between selenium and sulfur in plants and soils is important to study as it affects selenium nutrition and toxicity, crop composition and production, and the risk of toxicity to humans and animals. This study evaluated the impact of selenium exposure on rice plants grown under different sulfur supplies at different growth stages. The results showed that sulfate supply alleviated selenate toxicity, while soil clay content influenced selenate availability and adsorption capacity.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Lingshuang Wang, Haiyang Li, Milan He, Lidong Dong, Zerong Huang, Liyu Chen, Haiyang Nan, Fanjiang Kong, Baohui Liu, Xiaohui Zhao
Summary: Soybean is a photoperiod-sensitive crop with duplicated genes, including the important E2 maturity gene and its homologs. Through genome editing, it was found that E2 plays a major role in regulating flowering time and yield, while the two homologs depend on E2 function. Epistatic genes from the E1 transcription factor family were found to be related to E2. E2 and E2-like proteins form homodimers or heterodimers to regulate the transcription of E1 family genes, with the homodimer having a greater function. The ancestral allele H3 of E2 is mainly restricted to low latitude regions and has a stronger regulation of flowering time than the H1 haplotype.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Yuqi Wang, Shaohua Yang, Chune Li, Taijiao Hu, Siyu Hou, Qing Bai, Xiyue Ji, Feng Xu, Chongdai Guo, Min Huang, Yanfei Cai, Jiping Liu
Summary: In this study, the function of OsNIP1;2, a gene in rice, in aluminum detoxification was characterized. OsNIP1;2 was found to be specifically induced by aluminum stress in the roots and played a role in internal aluminum detoxification by regulating aluminum accumulation in the cell wall, cytosol, and distribution between roots and above-ground tissues.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Wendy J. Lyzenga, Zhigang Liu, Toluwase Olukayode, Yang Zhao, Leon V. Kochian, Byung-Kook Ham
Summary: Enhancing nutrient use efficiency in agriculture requires understanding the molecular features of nutrient uptake by roots, root system architecture, and crop nutrient use efficiency. The main source of essential mineral nutrients for plants is the soil, which plays a crucial role in plant biomolecule synthesis, cell processes, and enzymatic reactions. The roots serve as the main entry point for mineral nutrients, and various transport systems, sensors, and signaling proteins facilitate their uptake. Chemical fertilizers containing nitrogen, phosphorus, and potassium are commonly used to maximize crop yields. However, optimizing nutrient uptake and utilization is essential for sustainable agriculture. Therefore, this review focuses on the molecular basis of N, P, and K nutrient uptake, the relationship between root system architecture and nutrient distribution, and the coordination of nutrient uptake with photosynthesis through the xylem and phloem. The size, structure, and activity of the root system, along with nutrient transporters, determine the rate of nutrient acquisition. The connections between N, P, and K uptake and signaling are also discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Natalia Trajano de Oliveira, Filipe Aiura Namorato, Sombir Rao, Arnon Afonso de Souza Cardoso, Pedro Milanez de Rezende, Luiz Roberto Guimaraes Guilherme, Jiping Liu, Li Li
Summary: Zinc (Zn) and iron (Fe) are essential micronutrients for all living organisms and major targets for crop biofortification. Excess Zn can induce Fe deficiency symptoms in soybean plants, while Fe accumulation is not greatly affected by elevated Zn supplies. The interaction between Zn and Fe also influences the activities of antioxidative enzymes and the uptake and homeostasis of other essential micronutrients in soybean plants.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Entomology
Jine Chen, Xin Du, Xia Xu, Sheng Zhang, Lusong Yao, Xiuling He, Yongqiang Wang
Summary: Parthenogenesis is a common reproductive mode in insects, but its molecular mechanism remains to be elucidated. The silkworm Bombyx mori is an economically important insect and artificial parthenogenesis via thermal stimuli is widely used in sericultural production. In this study, iTRAQ-based proteomics were used to compare the protein expression profile between a fully parthenogenetic line (PL) and its parent amphigenetic line (AL). The results provide new insights into the molecular mechanism underlying silkworm parthenogenesis.
Article
Food Science & Technology
Li Min, Gang Wang, Xiong Tong, Huaigu Yang, Hao Sun, Zhifei Zhang, Bin Xu, Dagang Li, Sheng Zhang, Guanghong Li
Summary: This study showed that adding Aquilaria sinensis leaves to goats' diet did not affect their growth performance but significantly reduced feeding costs. It also found that goats fed with A. sinensis leaves had lower blood cholesterol levels and higher levels of high-density lipoprotein. Metabolomics analysis further confirmed the reduction in cholesterol levels in both the serum and muscle of the goats. These observed changes contribute to improving animal health and meat quality, ultimately benefiting human health.
Article
Biochemistry & Molecular Biology
Tianhu Sun, Peng Wang, Sombir Rao, Xuesong Zhou, Emalee Wrightstone, Shan Lu, Hui Yuan, Yong Yang, Tara Fish, Theodore Thannhauser, Jiping Liu, Michael Mazourek, Bernhard Grimm, Li Li
Summary: This study discovered that OR family proteins coordinate the biosynthesis pathways of chlorophylls and carotenoids at the posttranslational level and stabilize related enzymes. Loss of OR genes hinders the biosynthesis of both chlorophylls and carotenoids, affecting the stacking of grana in chloroplasts. Overexpression of OR enhances photosynthetic pigment biosynthesis and thermotolerance in plants.
Article
Plant Sciences
Arnon Afonso de Souza Cardoso, Filipe Aiura Namorato, Luiz Roberto Guimaraes Guilherme, Maria Ligia de Souza Silva, Jiping Liu, Li Li
Summary: This study investigates the role of glutathione (GSH) in resistance to selenium (Se) toxicity in plants, as well as its effects on gene expression and growth. The results demonstrate the critical role of GSH in detoxifying Se and provide insights into metal tolerance for Se biofortification in crops.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
Article
Food Science & Technology
Wancong Yu, Shihao Li, Bowen Zheng, Yuqi Wang, Yue Yu, Yumeng Wang, Xu Zheng, Jiping Liu, Zhijun Zhang, Zhaohui Xue
Summary: In this study, the effect of polyethylene (PE) packaging on the lignification of Pleurotus eryngii was investigated. The results showed that PE packaging delayed lignification by inhibiting lignin synthesis and up-regulating genes involved in lignin decomposition. This study provides valuable insights for improving the quality of edible fungi and postharvest storage.
FOOD CHEMISTRY: MOLECULAR SCIENCES
(2022)