期刊
PLANT JOURNAL
卷 97, 期 3, 页码 543-554出版社
WILEY
DOI: 10.1111/tpj.14141
关键词
lignocellulose; genome editing; Oryza sativa; S/G ratio; grass; p-coumarate; NMR; DFRC
资金
- Japan Science and Technology Agency/Japan International Cooperation Agency (Science and Technology Research Partnership for Sustainable Development, SATREPS)
- Japan Society for the Promotion of Science (KAKENHI) [16K07806, 16K14958, 16H06198]
- RISH, Kyoto University [2016-5-2-1]
- JSPS fellowship program [17J09654]
- Grants-in-Aid for Scientific Research [16K07806] Funding Source: KAKEN
The aromatic composition of lignin is an important trait that greatly affects the usability of lignocellulosic biomass. We previously identified a rice (Oryza sativa) gene encoding coniferaldehyde 5-hydroxylase (OsCAld5H1), which was effective in modulating syringyl (S)/guaiacyl (G) lignin composition ratio in rice, a model grass species. Previously characterized OsCAld5H1-knockdown rice lines, which were produced via an RNA-interference approach, showed augmented G lignin units yet contained considerable amounts of residual S lignin units. In this study, to further investigate the effect of suppression of OsCAld5H1 on rice lignin structure, we generated loss-of-function mutants of OsCAld5H1 using the CRISPR/Cas9-mediated genome editing system. Homozygous OsCAld5H1-knockout lines harboring anticipated frame-shift mutations in OsCAld5H1 were successfully obtained. A series of wet-chemical and two-dimensional NMR analyses on cell walls demonstrated that although lignins in the mutant were predictably enriched in G units all the tested mutant lines produced considerable numbers of S units. Intriguingly, lignin gamma-p-coumaroylation analysis by the derivatization followed by reductive cleavage method revealed that enrichment of G units in lignins of the mutants was limited to the non-gamma-p-coumaroylated units, whereas grass-specific gamma-p-coumaroylated lignin units were almost unaffected. Gene expression analysis indicated that no homologous genes of OsCAld5H1 were overexpressed in the mutants. These data suggested that CAld5H is mainly involved in the production of non-gamma-p-coumaroylated S lignin units, common in both eudicots and grasses, but not in the production of grass-specific gamma-p-coumaroylated S units in rice.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据