期刊
PLANT AND CELL PHYSIOLOGY
卷 60, 期 1, 页码 126-138出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pcy193
关键词
Autophagy; Chlamydomonas reinhardtii; Green alga; Maternal inheritance; Starch; Triacylglycerol
资金
- Japan Society for the Promotion of Science [16H04805]
- Japan Science and Technology Agency [Advanced Low Carbon Technology Research and Development Program] [JPMJAL1105]
- New Energy and Industrial Technology Development Organization (NEDO) of Japan [P10010]
- Grants-in-Aid for Scientific Research [16H04805] Funding Source: KAKEN
Autophagy is a recycling system for amino acids and carbon-and nitrogen (N)-containing compounds. To date, the functional importance of autophagy in microalgae in nutrient-deficient conditions has not been evaluated by using autophagy-defective mutants. Here, we provide evidence which supports the following notions by characterizing an insertional mutant of the autophagy-related gene ATG8, encoding a ubiquitin-like protein necessary for the formation of the autophagosome in the green alga, Chlamydomonas reinhardtii. First, ATG8 is required for maintenance of cell survival and Chl content in N-, sulfur- and phosphate-deficient conditions. Secondly, ATG8 supports the degradation of triacylglycerol and lipid droplets after the resupply of N to cells cultured in N-limiting conditions. Thirdly, ATG8 is also necessary for accumulation of starch in phosphate-deficient conditions. Additionally, autophagy is not essential for maternal inheritance of the organelle genomes in gametogenesis.
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