Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Laidi Wu, Zhixin Liu, Li Xiao, Mi Ai, Yingguang Cao, Jing Mao, Ke Song
Summary: This review comprehensively elucidates the specific roles of Gli1+ MSCs in craniofacial bone osteogenesis and tissue repair, offering new insights into bone regeneration therapy.
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Endocrinology & Metabolism
Sheng Zheng, Chunhao Zhou, Han Yang, Junhua Li, Ziyu Feng, Liqing Liao, Yikai Li
Summary: Previous studies have shown that melatonin can promote both osteogenesis and angiogenesis. This study aimed to investigate the effects of melatonin on angiogenesis and osteoporotic bone defect. The results demonstrated that melatonin treatment increased the expression of osteogenesis-related and angiogenesis-related markers in bone marrow mesenchymal stem cells (BMSCs). Additionally, melatonin promoted angiogenesis mediated by BMSCs by upregulating VEGF levels. Furthermore, melatonin treatment improved bone mineralization and formation around tibia defects in osteoporotic rats, as well as increasing bone strength. These findings suggest that melatonin may have potential applications in the treatment of osteoporotic bone defects.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Engineering, Biomedical
Qij Lu, Jingjing Diao, Yingqu Wang, Jianlang Feng, Fansen Zeng, Yan Yang, Yudi Kuang, Naru Zhao, Yingjun Wang
Summary: By evaluating the cell behaviors of bone mesenchymal stem cells (BMSCs) on beta-tricalcium phosphate (β-TCP) scaffolds with different pore morphologies, it was found that the β-TCP scaffold with a diamond pore unit (D-scaffold) enhanced cell cytoskeletal forces, elongated nucleus, increased cell mobility, and promoted osteogenic differentiation potential. The involvement of the Ras homolog gene family A (RhoA)/Rho-associated kinase-2 (ROCK2) signaling pathway in the pore morphology-mediated BMSCs behaviors was revealed, highlighting the importance of mechanical signaling transduction in scaffold-cell interactions. Furthermore, femoral condyle defect repair results demonstrated that D-scaffold effectively promoted endogenous bone regeneration.
BIOACTIVE MATERIALS
(2023)
Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Ting-Wei Kao, Arthur Chiou, Keng-Hui Lin, Yi-Shiuan Liu, Oscar Kuang-Sheng Lee
Summary: Research shows that mechanical stimulation affects the intracellular viscoelasticity of human mesenchymal stem cells, with cells cultured in rigid matrix displaying higher elastic and viscous moduli. This effect remains consistent throughout the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Jingwen Zhuang, Ruiyue Hang, Ruoyue Sun, Yanshu Ding, Xiaohong Yao, Ruiqiang Hang, Hui Sun, Long Bai
Summary: Exosomes secreted by bone marrow mesenchymal stem cells have multifaceted effects on osseointegration and hold great promise as a safe, efficient, and ethically unrestricted therapy.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Long Chen, Wei Luo, Yuanzheng Wang, Xiongbo Song, Senlei Li, Jun Wu, Li Sun
Summary: This study demonstrates that glycosylation modification enhances the directional homing ability of BMSCs to bone defect sites by enabling them to express high levels of sLe(X) antigen. Results show that glycosylation-modified BMSCs can improve the repair of bone defects in mice.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Polymer Science
Chong Teng, Zhicheng Tong, Qiulin He, Huangrong Zhu, Lu Wang, Xianzhu Zhang, Wei Wei
Summary: In this study, biocompatible hydrogel microspheres were fabricated to promote the osteogenic differentiation of stem cells and enhance bone regeneration. The results from in vivo experiments demonstrated the superiority of the BMSC/HM system in inducing bone regeneration. This study provides a simple and effective research idea for bone defect repair, and the optimization of hydrogel microspheres holds promise for future tissue engineering applications.
Review
Cell Biology
Xinyun Xu, Ling Zhao, Paul D. Terry, Jiangang Chen
Summary: Mesenchymal stem cells from bone marrow can differentiate into either adipocytes or osteoblasts, and external stimuli such as environmental contaminants, heavy metals, dietary, and physical factors can influence this fate decision. Maintaining a balance between osteogenesis and adipogenesis is crucial for bone homeostasis, and disruptions in lineage commitment of these stem cells are associated with various bone disorders. This review focuses on understanding how external stimuli affect the fate of mesenchymal stem cells and calls for future studies to investigate the impact on bone health and underlying mechanisms of differentiation.
Article
Biochemistry & Molecular Biology
Wei Zhang, Zhiwen Jiang, Jinhua Chi, Huanchao Sun, Hongjian Li, Wanshun Liu, Baoqin Han
Summary: Bone defects are a common problem in orthopedics and pose a serious threat to human health. Synthetic cell-free functionalized scaffolds, specifically butyryl chitin (BC), have been investigated for their potential in bone tissue engineering. BC films showed excellent tensile strength and hydrophobicity, promoting mineral deposition, and in vitro and in vivo tests confirmed their biocompatibility. By combining BC with hydroxyapatite (HA), a composite scaffold with good pore structure and mechanical strength was created, which effectively promoted new bone regeneration in rat skull defects. These findings demonstrate the successful development of a BC-HA porous scaffold as a potential substitute for bone transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Tong Wang, Zhou Yu, Yajuan Song, Liu Yang, Lin Chen, Juanli Dang, Xianhui Zeng, Shuzhong Guo, Yingjun Su, Baoqiang Song
Summary: The effect of the dura mater on the integration of porous titanium implants and cranial bone was evaluated in this study. It was found that the group with intact dura mater showed significantly higher bone volume/total volume and trabecular number. Histological analysis also revealed more newly formed bone and calcification deposition in this group. These findings indicate that having an intact dura mater improves the osteogenesis and osteointegration properties of porous titanium implants.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Patricia Garcia-Garcia, Ricardo Reyes, Maria Isabel Perez-Nunez, Jose A. Riancho, Carmen Evora, Jose Carlos Rodriguez-Rey, Flor M. Perez-Campo
Summary: The study demonstrates a method to enhance the osteogenic potential of MSCs by modulating the expression levels of specific genes. It was found that using Locked Nucleic Acid-Antisense Oligonucleotides and a scaffold releasing low doses of BMP-2 can increase the osteogenic potential of MSCs. By specifically targeting the Wnt pathway modulator Sfrp1, the efficiency of MSC bone regeneration can be improved and osteogenic differentiation in osteoporotic patients' MSCs can be induced.
Article
Engineering, Environmental
Sita Shrestha, Seo Yeon Lee, Devendra Shrestha, Rupesh Kandel, Yeo-Jin Yoo, Hyun-Jin Tae, Bishnu Kumar Shrestha, Chan Hee Park, Cheol Sang Kim
Summary: This work describes the synthesis of an ideal bioceramic that closely resembles bone-like apatite in geometry and composition, and can easily stimulate cellular response. The bioceramic, with an optimized weight percentage of zinc, shows potential for bone defect repair and bone void filling in various clinical approaches. The detailed physicochemical and biological characterizations reveal that the bioceramic possesses a biocompatible and porous structure similar to hydroxyapatite, and stimulates bone regeneration by enhancing cell viability and osteogenic differentiation. The findings also demonstrate the effectiveness of the bioceramic in promoting new bone growth in a rat model with critical size calvarial defect. This study provides valuable insights into the osteogenesis of the (Ti(HPO4)(2)/Zn-3(PO4)(2)·nH2O) bioceramic and contributes to the development of therapeutic strategies in bone tissue regenerative medicine.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Yonggang Dang, Li Zhang, Wen Song, Bei Chang, Tianxiao Han, Yumei Zhang, Lingzhou Zhao
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2016)
Article
Chemistry, Multidisciplinary
Tian-Xiao Han, Bei Chang, Xin Ding, Guang-Na Yue, Wen Song, Hui-Ping Tang, Liang Jia, Ling-Zhou Zhao, Yu-Mei Zhang
Article
Engineering, Biomedical
Mengqi Shi, Wen Song, Tianxiao Han, Bei Chang, Guangwen Li, Jianfeng Jin, Yumei Zhang
ACTA BIOMATERIALIA
(2017)