期刊
ACTA BIOMATERIALIA
卷 44, 期 -, 页码 168-177出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2016.08.042
关键词
Strontium borosilicate glasses; PLLA membranes; Mineralisation; Osteogenic differentiation; BM-MSCs
资金
- Portuguese Foundation for Science and Technology [BD/73162/2010]
- European Union [REGPOT-CT2012-31633-POLARIS]
- European Research Council [ERC-2012-ADG-20120216-321266]
- UK EPSRC Centre for Innovative Manufacturing of Medical Devices-MeDe Innovation (EPSRC) [EP/K029592/1]
- EPSRC [EP/K029592/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/K029592/1] Funding Source: researchfish
Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass particles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (mu-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1-3 mu m in width) with a homogeneous distribution of BBG-Sr microparticles (<45 mu m Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membrane's mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr membranes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomaterial capable of promoting bone regeneration. Statement of Significance PLLA membranes were reinforced with 10% (w/w) of strontium-bioactive borosilicate glass micropartides, and their capacity to induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was evaluated. These membranes presented an increased: degradability, water uptake, Young modulus and tensile strength. We also demonstrated that these membranes are non-cytotoxic and promote the attachment of BM-MSCs. The addition of the glass microparticles into the PLLA membranes promoted the increase of ALP activity (under osteogenic conditions), as well as the BM-MSCs osteogenic differentiation as shown by the upregulation of Alpl, Sp7 and Bglap gene expression. Overall, we demonstrated that the reinforcement of PLLA with glass microparticles results in a biomaterial with the appropriate properties for the regeneration of bone tissue. (C) 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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