期刊
NEW PHYTOLOGIST
卷 221, 期 3, 页码 1518-1528出版社
WILEY
DOI: 10.1111/nph.15475
关键词
14-3-3; aphid effector; Macrosiphum euphorbiae; Me10; TFT7; tomato (Solanum lycopersicum); virulence target
资金
- University of California Experimental Station
- Binational Science Foundation [2011069, 2015062]
- Guru Gobind Singh Fellowship Award from University of California
- Direct For Biological Sciences
- Division Of Environmental Biology [2011069] Funding Source: National Science Foundation
We demonstrated previously that expression of Macrosiphum euphorbiae salivary protein Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However, the mechanism of action of Me10 remained elusive. To confirm the secretion of Me10 by the aphid into plant tissues, we produced Me10 polyclonal antibodies. To identify the plant targets of Me10, we developed a tomato immune induced complementary DNA yeast two-hybrid library and screened it with Me10 as bait. Immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays were performed to validate one of the interactions in planta, and virus-induced gene silencing was used for functional characterization in tomato. We demonstrated that Me10 is secreted into the plant tissues and interacts with tomato 14-3-3 isoform7 (TFT7) in yeast. Immunoprecipitation assays confirmed that Me10 and its homologue in Aphis gossypii, Ag10k, interact with TFT7 in planta. Further, BiFC revealed that Me10 interaction with TFT7 occurs in the plant cell cytoplasm. While silencing of TFT7 in tomato leaves did not affect tomato susceptibility to M.euphorbiae, it enhanced longevity and fecundity of A.gossypii, the non-host aphid. Our results suggest the model whereby TFT7 plays a role in aphid resistance in tomato and effectors of the Me10/Ag10k family interfere with TFT7 function during aphid infestation.
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