Article
Biochemical Research Methods
Lingjun Ding, Giuseppe Balsamo, Maria Diamantaki, Patricia Preston-Ferrer, Andrea Burgalossi
Summary: Neural circuits are made up of various types of neuronal cells and understanding how this diversity contributes to brain function during behavior has been a major challenge. This study presents a protocol for performing juxtacellular opto-tagging of single neurons in freely moving mice, allowing for selective targeting of specific cell classes for in vivo single-cell recordings. The protocol can be completed in approximately 4-5 weeks.
Article
Engineering, Biomedical
Yonghee Chang, Juntae Jang, Jaeouk Cho, Jingu Lee, Yeonzu Son, Seongjun Park, Chul Kim
Summary: This research presents a seamless capacitive body channel wireless power transmission system that enables stable power delivery to multiple living animals. The system is designed to allow long-term and real-time neural monitoring in preclinical research. Experimental results show that the power efficiency remains stable in diverse scenarios, with a maximum difference of 6.66% even with a 90 degrees rotation of the receiver against the cage. In-vivo experiments conducted with untethered rats demonstrate the system's capability of continuous long-term power delivery in practical situations.
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2022)
Article
Optics
Arutyun Bagramyan, Loic Tabourin, Ali Rastqar, Narges Karimi, Frederic Bretzner, Tigran Galstian
Summary: This study introduces a novel miniature single-photon microscope with an electrically tunable liquid crystal lens for imaging in-depth fine neuronal structures in the brains of freely moving mice. The microscope is compact, lightweight, offers fast acquisition, high magnification, and high resolution, allowing for imaging of calcium activity during a wide range of behavioral tasks.
PHOTONICS RESEARCH
(2021)
Article
Chemistry, Physical
Jung-uk Lee, Wookjin Shin, Yongjun Lim, Jungsil Kim, Woon Ryoung Kim, Heehun Kim, Jae-Hyun Lee, Jinwoo Cheon
Summary: m-Torquer is a magnetic toolkit that mimics magnetoreception in nature, providing precise stimulation to cells and reliable neuromodulation in animals like mice. Its versatility allows for a wide range of applications, potentially even in large animals such as primates.
Article
Zoology
Xinsong Guo, Shan Li, Xuejie Yu, Tingting Wu, Penglai Liu, Yufeng Shao, Anan Li
Summary: The article introduces a new odor stimulation system that can precisely present odors in freely moving mice. The system can be combined with neural recordings and olfactory behavioral tests to investigate how neurons in the brain represent odor information during individual olfactory behaviors.
INTEGRATIVE ZOOLOGY
(2023)
Article
Multidisciplinary Sciences
Arnaud Landra-Willm, Ameya Karapurkar, Alexia Duveau, Anne Amandine Chassot, Lucille Esnault, Gerard Callejo, Marion Bied, Stephanie Hafner, Florian Lesage, Brigitte Wdziekonski, Anne Baron, Pascal Fossat, Laurent Marsollier, Xavier Gasull, Eric Boue-Grabot, Michael A. Kienzler, Guillaume Sandoz
Summary: By manipulating neuronal activity using optogenetics and photopharmacology, researchers have developed a light-activatable molecule called LAKI that can block pain-related potassium channels. This molecule has the potential to be a valuable tool for studying these channels, and it can also be used to remotely control pain in animal models without the need for genetic manipulations or viral infection. This non-invasive and reversible approach has applications in pain research and drug screening.
NATURE COMMUNICATIONS
(2023)
Article
Neurosciences
Kang Huang, Qin Yang, Yaning Han, Yulin Zhang, Zhiyi Wang, Liping Wang, Pengfei Wei
Summary: Measuring eye movement is important in cognitive science, and this study introduces a novel system that combines hardware and algorithms to analyze complex and dynamic eye-tracking data in freely-moving animals. This system provides a feasible approach to understanding the neural substrates of cognitive function.
NEUROSCIENCE BULLETIN
(2022)
Article
Multidisciplinary Sciences
Guangfu Wu, Ian Heck, Nannan Zhang, Glenn Phaup, Xincheng Zhang, Yixin Wu, David E. Stalla, Zhengyan Weng, He Sun, Huijie Li, Zhe Zhang, Shinghua Ding, De-Pei Li, Yi Zhang
Summary: This study introduces a wireless, programmable push-pull microsystem for membrane-free neurochemical sampling with cellular spatial resolution in freely moving animals. It shows high efficiency in sampling various neurochemicals and captures the release of neuropeptides in freely moving mice. This system provides opportunities for understanding the modulation of diverse behavioral outputs of the brain by neuropeptide release.
Article
Multidisciplinary Sciences
Aghileh S. Ebrahimi, Patrycja Orlowska-Feuer, Qian Huang, Antonio G. Zippo, Franck P. Martial, Rasmus S. Petersen, Riccardo Storchi
Summary: The 3D-UPPER algorithm allows for accurate 3D reconstruction of freely moving animals, with the advantages of being fully automated, requiring no prior knowledge, and applicable to 2D data.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Jokubas Ausra, Mingzheng Wu, Xin Zhang, Abraham Vazquez-Guardado, Patrick Skelton, Roberto Peralta, Raudel Avila, Thomas Murickan, Chad R. Haney, Yonggang Huang, John A. Rogers, Yevgenia Kozorovitskiy, Philipp Gutruf
Summary: Wireless, battery-free, and fully subdermally implantable optogenetic tools have the potential to revolutionize neurobiological research in freely moving animals, but current devices still have issues with invasive stimulus delivery causing damage during implantation, as well as power delivery constraints limiting the size of operational arenas.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Neurosciences
Kunpeng Chen, Zhaoshi Tian, Lingjie Kong
Summary: This review discusses the importance of optical miniscopes in studying neural activity in freely moving animals, including their fundamental principles, system structures, technical advances, limitations, and future trends.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Fushun Wang, Wei Wang, Simeng Gu, Dan Qi, Nathan A. Smith, Weiguo Peng, Wei Dong, Jiajin Yuan, Binbin Zhao, Ying Mao, Peng Cao, Qing Richard Lu, Lee A. Shapiro, S. Stephen Yi, Erxi Wu, Jason H. Huang
Summary: The researchers discovered two distinct astrocytic Ca2+ signals in the mouse barrel cortex, which play different roles in modulating sensory transmission during sleep and arousal states.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Nikolas Perentos, Marino Krstulovic, A. Jennifer Morton
Summary: Sheep, as a practical large animal species, were used for in vivo brain function studies in naturalistic settings. The experiments demonstrated that sheep are excellent for longitudinal studies requiring large-brained mammals and/or large-scale recordings across distributed neuronal networks. This suggests that sheep can be safely used for studying neural encoding of decision-making and spatial-mapping in naturalistic environments, as well as the neural basis of intra- and inter-species social interactions.
Article
Chemistry, Multidisciplinary
Bingqian Zhou, Kuikui Fan, Jiazhen Zhai, Cheng Jin, Lingjie Kong
Summary: Brain temperature is crucial for neural activity and function. A new sensor, UCNP-FMS, loaded with UCNPs provides high spatial resolution for temperature monitoring in the deep brains of freely moving animals. The UCNP-FMS demonstrates superior performance in real-time temperature monitoring in different brain regions.
Article
Chemistry, Multidisciplinary
Yuandong Liu, Zhichao Liu, Fan Zhao, Yang Tian
Summary: Understanding physiological and pathological processes in the brain requires tracking reversible changes in chemical signals with long-term stability. A new anti-biofouling microfiber array was developed to quantify extracellular Ca2+ concentrations and neuron activity in real-time across multiple regions in the mammalian brain for 60 days, providing high tempo-spatial resolution and the ability to monitor reversible changes during ischemia-reperfusion processes. This microarray serves as a versatile tool for investigating brain dynamics during pathological processes and drug treatment, demonstrating the significant influence of ROS on Ca2+ overload and neuron death after stroke.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Robert Egger, Arno C. Schmitt, Damian J. Wallace, Bert Sakmann, Marcel Oberlaender, Jason N. D. Kerr
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Editorial Material
Biochemistry & Molecular Biology
Damian J. Wallace, David Fitzpatrick, Jason N. D. Kerr
Editorial Material
Biochemistry & Molecular Biology
Damian J. Wallace, Jason N. D. Kerr
Review
Neurosciences
Jason N. D. Kerr, Axel Nimmerjahn
CURRENT OPINION IN NEUROBIOLOGY
(2012)
Article
Multidisciplinary Sciences
Damian J. Wallace, David S. Greenberg, Juergen Sawinski, Stefanie Rulla, Giuseppe Notaro, Jason N. D. Kerr
Article
Neurosciences
Wolfgang Mittmann, Damian J. Wallace, Uwe Czubayko, Jan T. Herb, Andreas T. Schaefer, Loren L. Looger, Winfried Denk, Jason N. D. Kerr
NATURE NEUROSCIENCE
(2011)
Article
Biology
Verena Pawlak, David S. Greenberg, Henning Sprekeler, Wulfram Gerstner, Jason N. D. Kerr
Article
Biochemical Research Methods
Alexandr Klioutchnikov, Damian J. Wallace, Michael H. Frosz, Richard Zeltner, Juergen Sawinski, Verena Pawlak, Kay-Michael Voit, Philip St. J. Russell, Jason N. D. Kerr
Article
Multidisciplinary Sciences
Vishnudev Ramachandra, Verena Pawlak, Damian J. Wallace, Jason N. D. Kerr
NATURE COMMUNICATIONS
(2020)
Article
Biology
Carl D. Holmgren, Paul Stahr, Damian J. Wallace, Kay-Michael Voit, Emily J. Matheson, Juergen Sawinski, Giacomo Bassetto, Jason N. D. Kerr
Summary: The study found that mice primarily use a specific area of their visual field when capturing insects, and track the prey through head movements rather than eye movements.
Article
Biochemical Research Methods
Patrick Rose, Alexandr Klioutchnikov, Damian J. Wallace, David S. Greenberg, Jason N. D. Kerr, Juergen Sawinski
Summary: This paper presents a technique that reconstructs the three-dimensional scan path using only the translation of a simple fluorescent test probe, applicable to scanning instruments with sectioning capabilities. The method allows for analysis of scanning errors and restoration of anatomical accuracy, enhancing spatial registration and feature extraction.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biochemical Research Methods
Arne Monsees, Kay-Michael Voit, Damian J. Wallace, Juergen Sawinski, Edyta Charyasz, Klaus Scheffler, Jakob H. Macke, Jason N. D. Kerr
Summary: To understand the relationship between neural activity and skeletal kinematics, this study developed a videography-based method to quantify the three-dimensional kinematics of the skeletal system in freely behaving rats and mice. The anatomically defined skeleton model was constrained using anatomical principles and joint motion limits, enabling accurate reconstruction of complex decision-making behaviors at the level of skeletal kinematics.
Article
Cell Biology
Yunfeng Hua, Sahil Loomba, Verena Pawlak, Kay-Michael Voit, Philip Laserstein, Kevin M. Boergens, Damian J. Wallace, Jason N. D. Kerr, Moritz Helmstaedter
Summary: Thalamic input drives a subset of interneurons with high specificity, which in turn target excitatory neurons with subtype specificity, creating a directed disinhibitory network directly driven by thalamic input.
Article
Biochemical Research Methods
Alexandr Klioutchnikov, Damian J. Wallace, Juergen Sawinski, Kay-Michael Voit, Yvonne Groemping, Jason N. D. Kerr
Summary: A lightweight three-photon miniature microscope has been developed to image neuronal activity in the cortex of freely moving mice. The microscope allows access to all cortical layers while the mice freely behave in a fully lit environment. By comparing activity in cortical layer 4 and layer 6, the study shows that neuronal activity is differentially modulated by lit and dark conditions during free exploration.
