Review
Chemistry, Multidisciplinary
Yongzheng Xing, Alexia Rottensteiner, Jonah Ciccone, Stefan Howorka
Summary: Membrane-spanning nanopores have diverse applications in label-free single-molecule sensing, nucleic acid sequencing, and research in biology and biophysics. The limitations of naturally occurring and synthetic inorganic nanopores can be overcome by functionalizing existing pores with DNA strands and creating a new class of DNA nanopores. This review focuses on the progress and potential applications of functional DNA nanopores.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Saanfor Hubert Suh, Yongzheng Xing, Alexia Rottensteiner, Rong Zhu, Yoo Jin Oh, Stefan Howorka, Peter Hinterdorfer
Summary: This study investigates molecular recognition in a synthetic confined nanopore with controllable pore dimension and molecular DNA receptors at different depth positions. The results show that the association rates are slower and the dissociation rates are faster for receptors positioned deeper inside the pore lumen. This phenomenon can be explained by the steric constraints on molecular interactions in confinement.
Article
Chemistry, Multidisciplinary
Feng Li, Zhaoyue Lv, Xue Zhang, Yuhang Dong, Xiaohui Ding, Zhemian Li, Shuai Li, Chi Yao, Dayong Yang
Summary: This study reports a supramolecular self-assembled DNA nanosystem that reprogrammed the cellular antioxidant system through synergistic chemical and gene regulations, significantly promoting metabolic reprogramming of cancer cells, showing great potential in cancer therapy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Kuiting Chen, Chun Xie, Zhekun Chen, Shichen Wang, Yingxin Hu, Fei Xu, Linqiang Pan
Summary: Reconfigurable DNA origami allows for manipulation of matter on the nanometer scale, with the DNA kirigami method enabling the transformation of origami structures. However, constructing finely trimmed sub-origami structures has proven challenging due to the densely packed DNA duplexes. In this study, a presketched DNA origami canvas was proposed, which enhanced the fineness of polymerase-driven DNA kirigami.
Article
Chemistry, Multidisciplinary
Samet Kocabey, Aslihan Ekim Kocabey, Roger Schneiter, Curzio Ruegg
Summary: DNA nanotechnology utilizes cholesterol-modified DNA nanotubes to interact with cancer cells, increasing cellular membrane permeability and inducing cell death. This process partially depends on caspase activity and exhibits characteristics of both apoptotic and necrotic cell death. DNA nanotubes may have potential as cytotoxic agents or drug delivery vehicles through cholesterol-mediated cellular interactions.
Article
Chemistry, Multidisciplinary
Christopher M. Green, William L. Hughes, Elton Graugnard, Wan Kuang
Summary: The study utilized correlative microscopy techniques to investigate DNA nanostructures, finding that over 75% of defective docking sites were incorporated but inactive, with unincorporated strands playing a minor role in limiting the availability of addressable sites. Further exploration of the effects of strand purification, UV irradiation, and photooxidation on availability provided insights into potential sources of defects and pathways to improving the fidelity of DNA nanostructures.
Article
Chemistry, Multidisciplinary
Zhaohui Qin, Yu Liu, Linghao Zhang, Jiajia Liu, Xin Su
Summary: This study demonstrates a DNA-based artificial dissipative system with a molecular timer, which allows for dynamic enzyme catalysis and DNA nanostructure disassembly. This provides a useful tool for the development of artificial reaction networks and intelligent nanostructures.
Review
Chemistry, Multidisciplinary
Micah Yang, David Bakker, Dyuti Raghu, Isaac T. S. Li
Summary: A single strand of DNA can fold into DNA origami, which has advantages and challenges compared to double-stranded DNA construction.
FRONTIERS IN CHEMISTRY
(2023)
Review
Pharmacology & Pharmacy
Morgana Souza Marques, Lorena Almeida Lima, Fernanda Poletto, Renata Vidor Contri, Irene Clemes Kulkamp Guerreiro
Summary: In recent years, there has been a growing interest in the development of paediatric formulations, and nanotechnology has been proposed as a beneficial tool. This review explored the literature on various types of nanostructures for treating paediatric diseases and found that nanotechnology has the potential to enhance therapeutic efficacy, improve drug taste, and enable targeted therapy. The most commonly studied nanostructures were polymeric nanoparticles, lipid-based nanocarriers, and polymeric micelles. Specific tests designed for paediatric formulations included efficacy and safety studies in animal models of paediatric diseases, as well as palatability verification.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Masayuki Endo
Summary: Micrometer-scale assembly of DNA nanostructures is a major challenge in the field of DNA nanotechnology. This study demonstrates the programmed assembly of 1D and 2D DNA origami on a lipid bilayer surface using specific DNA strands and cross-shaped DNA origami. Real-time observation of lattice formation and rearrangement was achieved using high-speed atomic force microscopy. The study also explores the reversible photo-controlled assembly and disassembly of DNA origami on a lipid bilayer surface. These findings offer new approaches for controlling nanostructure formation.
Article
Polymer Science
Matteo Sanviti, Lars Mester, Rainer Hillenbrand, Angel Alegria, DanielE. Martinez-Tong
Summary: In this study, nanostructured conducting PEDOT:PSS thin films were prepared using solvent vapor annealing (SVA) with tetrahydrofuran (THF) as the low boiling point solvent. Atomic Force Microscopy (AFM) was used to observe the development of distinct nanostructures with solvent exposure time. The physical properties of the nanostructures were evaluated through nanomechanical, nanoelectrical, and nanoinfrared (nano-FTIR) measurements. It was demonstrated that exposure to THF can effectively and selectively modify the surface nanostructure of PEDOT:PSS and thus its final properties. The nanoscale studies also provided evidence of molecular rearrangements during nanostructure fabrication, which is crucial for expanding the potential applications of this polymer in thermoelectric and optoelectronic devices.
Article
Chemistry, Applied
Shalini Sahani, Yogesh Chandra Sharma
Summary: Nanotechnology plays a significant role in improving the characteristics and applications of food ingredients in the food industry, with a focus on enhancing solubility and controlled release. Research highlights advancements in preparation methods of nanostructures for food additives and packaging materials, while also addressing the environmental impact and potential solutions.
Article
Engineering, Environmental
Tiying Suo, Muhammad Sohail, Siying Xie, Bingzhi Li, Yue Chen, Lihui Zhang, Xing Zhang
Summary: This article introduces state-of-the-art analytical methods for the detection of MC-LR based on DNA nanotechnology and provides critical perspectives on the challenges and development in this field.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Chemistry, Multidisciplinary
Lexun Li, Shuang Liu, Chunjuan Zhang, Zhenzhen Guo, Shuxuan Shao, Xiaodan Deng, Qiaoling Liu
Summary: DNA molecule has unique advantages in cell surface engineering due to its excellent programmability and biocompatibility. Recent progresses in DNA-based cell surface engineering have provided a reliable and feasible way to engineer cell surfaces with diverse DNA molecules and DNA nanostructures. The abundant form of DNA nanostructures has greatly expanded the toolbox of DNA-based cell surface engineering and led to various novel and fascinating applications. This review summarizes recent advances in DNA-based cell surface engineering and its biological applications, discussing the methods of immobilizing DNA molecules on cell surfaces, the utilization of DNA nanostructures and dynamic DNA nanotechnology, as well as the challenges and prospects of DNA-based cell surface engineering.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Nanoscience & Nanotechnology
Qufei Gu, Yehan Zhang, Huan H. Cao, Songtao Ye, Tao Ye
Summary: The research demonstrates that thiolated strands displayed on a DNA origami nanostructure can be transferred to a gold surface, offering a pathway towards forming complex single-molecule nanoarrays for elucidating the structure-function relationships of enzyme cascades and interfacial molecular recognition.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Physical
A. Zambrano, A. S. Zadorin, Y. Rondelez, A. Estevez-Torres, J. -C. Galas
JOURNAL OF PHYSICAL CHEMISTRY B
(2015)
News Item
Physics, Multidisciplinary
Andre Estevez-Torres
Article
Chemistry, Multidisciplinary
Anton S. Zadorin, Yannick Rondelez, Guillaume Gines, Vadim Dilhas, Georg Urtel, Adrian Zambrano, Jean-Christophe Galas, Andre Estevez-Torres
Editorial Material
Nanoscience & Nanotechnology
Andre Estevez-Torres, Yannick Rondelez
NATURE NANOTECHNOLOGY
(2017)
Article
Nanoscience & Nanotechnology
G. Gines, A. S. Zadorin, J. -C. Galas, T. Fujii, A. Estevez-Torres, Y. Rondelez
NATURE NANOTECHNOLOGY
(2017)
Article
Biochemical Research Methods
Anis Senoussi, Jonathan Lee Tin Wah, Yoshihiro Shimizu, Jerome Robert, Alfonso Jaramillo, Sven Findeiss, Ilka M. Axmann, Andre Estevez-Torres
ACS SYNTHETIC BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Georg Urtel, Marc Van der Hofstadt, Jean-Christophe Galas, Andre Estevez-Torres
Article
Multidisciplinary Sciences
Anis Senoussi, Shunnichi Kashida, Raphael Voituriez, Jean-Christophe Galas, Ananyo Maitra, Andre Estevez-Torres
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Chemistry, Multidisciplinary
Marc Van der Hofstadt, Jean-Christophe Galas, Andre Estevez-Torres
Summary: The study demonstrates the feasibility of enzyme-DNA molecular programs in extracellular media, which can deliver DNA inside living cells and remain functional for over 48 hours. This internalization program with nonequilibrium dynamics allows for control of both the time and position of cell internalization, further achieving spatially inhomogeneous internalization.
Article
Chemistry, Multidisciplinary
Yuliia Vyborna, Jean-Christophe Galas, Andre Estevez-Torres
Summary: Living cells move and change shape due to chemical reactions that modify their cytoskeleton. By using DNA-responsive surfaces to control an active gel made of microtubules, forces can be generated to structure the gel, forming periodic band patterns or global contractions depending on DNA sequence and concentration. The structuration of the active gel can be spatially controlled in the presence of a DNA concentration gradient, showing potential for the development of life-like materials with self-shaping properties.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Anis Senoussi, Jean-Christophe Galas, Andre Estevez-Torres
Summary: The study integrates reaction-diffusion and active matter mechanisms, controlling mechano-chemical coupling through dissipative DNA/enzyme reaction networks in an active gel, successfully designing a synthetic material mimicking the polarization mechanism in C. elegans oocytes.
Article
Chemistry, Physical
Guillaume Sarfati, Ananyo Maitra, Raphael Voituriez, Jean-Christophe Galas, Andre Estevez-Torres
Summary: This study observed four instabilities in an active gel and found that they are controlled by the concentrations of ATP and depletion agent. The results show that as the ATP concentration decreases, the gel undergoes a gelation transition, and buckling is selected against bending while global contraction is favored over local ones.
Article
Physics, Fluids & Plasmas
Clara del Junco, Andre Estevez-Torres, Ananyo Maitra
Summary: Spontaneous pattern formation in living systems is driven by the interplay between reaction-diffusion chemistry and active mechanics. In this study, a minimal model of coupling between reaction-diffusion and active matter is analyzed, revealing that the propagation of a front is maintained in active systems with distinct effects from different types of stress.
Article
Chemistry, Physical
Georg Urtel, Andre Estevez-Torres, Jean-Christophe Galas