Article
Medicine, General & Internal
Sinmanus Vimonpatranon, Sittiruk Roytrakul, Narumon Phaonakrop, Kittima Lekmanee, Anyapat Atipimonpat, Narinee Srimark, Kasama Sukapirom, Kesinee Chotivanich, Ladawan Khowawisetsut, Kovit Pattanapanyasat
Summary: In infectious diseases, EVs released from pathogen-infected cells can regulate immune responses and carry specific human proteins, while most pathogen proteins in the EVs are common across different types. Pf-MVs were found to attenuate parasite invasion efficiency, with a greater effect in culture-adapted isolates. This suggests that Pf-EVs play a role in quorum sensing and regulation of parasite growth-density.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Daniela Peruzzu, Zaira Boussadia, Federica Fratini, Francesca Spadaro, Lucia Bertuccini, Massimo Sanchez, Maria Carollo, Paola Matarrese, Mario Falchi, Francesca Iosi, Carla Raggi, Isabella Parolini, Alessandra Care, Massimo Sargiacomo, Maria Cristina Gagliardi, Katia Fecchi
Summary: In this study, the role of cholesterol transport in the endosomal degradative-secretory system was explored using the U18666A inhibitor in a metastatic human melanoma cell line (WM266-4). The results showed that U18666A induced a shift of Cav-1 from the plasma membrane to the endolysosomal compartment, affecting the formation and release of small extracellular vesicles (sEVs). The inhibitor also altered the protein composition of sEVs and reduced their transfer capacity on target cells.
Article
Cell Biology
Monique Fatmous, Alin Rai, Qi Hui Poh, Lois A. Salamonsen, David W. Greening
Summary: This study investigated the impact of sEVs released by endometrial epithelial cells on trophoblast invasion, revealing their ability to promote invasion and induce proteome and surfaceome changes in trophoblast cells to support embryo implantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jin-Mei Yao, Hua-Zhong Ying, Huan-Huan Zhang, Fen-Sheng Qiu, Jun-Qi Wu, Chen-Huan Yu
Summary: Exosomes enriched with retinol-binding protein 4 (RBP4) were found to be a potential diagnostic biomarker for nonalcoholic fatty liver disease (NAFLD). These exosomes enhanced the M1-like polarization of Kupffer cells (KCs) and resulted in the over-production of inflammatory cytokines. Furthermore, TNF-alpha played a positive role in the development of NAFLD by increasing RBP4 transcription and promoting lipid accumulation.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Cell Biology
Yangyang Li, Jian Zhang, Sha Li, Chongye Guo, Qian Li, Xin Zhang, Meng Li, Shuangli Mi
Summary: In this study, a loading pathway of batched tumor-promoting sEV-miRNAs in NSCLC cell line A549 was comprehensively illustrated, with hnRNPA1 identified as a key loading protein and CAV1 demonstrated to assist in sEV-miRNA loading. This loading mechanism sheds light on potential therapeutic targets for lung cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Wenyun Hou, Meng Pan, Yi Xiao, Wei Ge
Summary: In this study, quantitative proteomics was used to analyze serum-derived extracellular vesicles (EV) in colorectal cancer patients, revealing differences in proteomic profiles between PNI and NPNI groups. Stratifin (SFN) expression levels in EV were identified as a potential biomarker for distinguishing PNI from NPNI patients and as an independent predictor of CRC prognosis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Jolene Chisholm, Sandor Haas-Neill, Peter Margetts, Khalid Al-Nedawi
Summary: Prostate cancer (PC) is a major cause of death in men, and current biomarker tests have limitations in accurately diagnosing the disease. This study examined the protein, mRNA, and miRNA content of extracellular vesicles (EVs) collected from PC patients and healthy subjects. The analysis revealed distinct groups of proteins, mRNAs, and miRNAs that differed between healthy subjects and PC patients, as well as between different tumor types. These findings suggest potential molecular markers for diagnosing PC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ana Mencher, Miguel Mejias-Ortiz, Pilar Morales, Jordi Tronchoni, Ramon Gonzalez
Summary: Malolactic fermentation is crucial for the quality of red wines. This study reports the production and characterization of extracellular vesicles by Oenococcus oeni, a common bacteria involved in spontaneous malolactic fermentation. The findings contribute to the understanding of microbial interactions during wine fermentation.
Review
Biotechnology & Applied Microbiology
Shenglong Li
Summary: Bone sarcomas are rare cancers involving metastatic disease, with extracellular vesicles (EVs) playing a crucial role in communication between cells and serving as biomarker vehicles for diagnosis and prognosis. The review presents new insights into potential therapeutic targets and diagnostic analyses in bone sarcomas.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Zhuoyue Lei, Hongwei Jiang, Jie Liu, Yuping Liu, Di Wu, Chenwei Sun, Qijun Du, Liangwen Wang, Guohua Wu, Shuqi Wang, Xingdong Zhang
Summary: This study presents a novel method to enhance the biofabrication of cancer-derived extracellular vesicles (EVs) using audible acoustic wave (AAW), which significantly increases EV yield and promotes cancer cell migration and invasion. Additionally, AAW stimulation activates the ESCRT pathway and upregulates membrane fusion-associated proteins, contributing to the enhancement of EV biofabrication.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Rafaela Furioso Ferreira, Thomas Blees, Farhad Shakeri, Andreas Buness, Marc Sylvester, Giovanni Savoini, Alessandro Agazzi, Vladimir Mrljak, Helga Sauerwein
Summary: This study isolated exosomes from porcine milk in different lactation stages and analyzed their content using a TMT-based high-resolution quantitative proteomic approach. Different proteome profiles were revealed for colostrum exosomes and milk exosomes, with functional analysis highlighting pathways related to homeostasis in colostrum exosomes and pathways related to endothelial cell development and lipid metabolism in mature milk exosomes. These findings emphasize the importance of exosomes as active biocomponents of milk and provide insights for future studies on their role in immunity regulation and growth of newborns.
JOURNAL OF PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
Joanna Szuszkiewicz, Kamil Myszczynski, Zaneta P. Reliszko, Yael Heifetz, Monika M. Kaczmarek
Summary: Research has shown that during early pregnancy, communication between the embryo and uterus occurs through the exchange of extracellular vesicles carrying miRNAs. These vesicles impact embryonic development and trophoblast cell function and interactions, playing a crucial role in successful embryo implantation and pregnancy.
Article
Food Science & Technology
Bin Wang, Xin-Jian Guo, Heng Cai, Yu-Hua Zhu, Lin-Yan Huang, Wan Wang, Lan Luo, Su-Hua Qi
Summary: Momordica.charantia-derived extracellular vesicles-like nanovesicles (MCELNs) have the potential to treat glioma by inhibiting cell proliferation and migration, and may be associated with the PI3K/AKT pathway.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Amber Rose Hart, Norhayati Liaqat Ali Khan, Keerthie Dissanayake, Kasun Godakumara, Aneta Andronowska, Saji Eapen, Paul R. Heath, Alireza Fazeli
Summary: Successful embryo implantation and endometrial receptivity require communication between the embryo and the maternal endometrium, which is facilitated by extracellular vehicles (EVs). This study investigated the influence of menstrual cycle hormones (estrogen, progesterone, and estrogen plus progesterone) on endometrial-derived EVs. The results showed that hormonal stimulation affected the size and proteome of EVs, with proteins involved in embryo implantation, endometrial receptivity, and embryo development being enhanced in the EVs from the receptive phase (estrogen plus progesterone). This supports the concept of EV-mediated communication between the embryo and the maternal endometrium.
Review
Biochemistry & Molecular Biology
Rui Miguel Marques Bernardino, Ricardo Leao, Rui Henrique, Luis Campos Pinheiro, Prashant Kumar, Prashanth Suravajhala, Hans Christian Beck, Ana Sofia Carvalho, Rune Matthiesen
Summary: The study explores possible strategies for urinary biomarker discovery, especially focused on proteome of urine EVs for PCa. Through reanalysis of proteomics data and network analysis, the findings suggest that urinary EVs may provide potential biomarkers with higher protein coverage compared to other sample sources and may fully characterize the heterogeneity of PCa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Rodrigo A. Acuna, Manuel Varas-Godoy, Diego Herrera-Sepulveda, Mauricio A. Retamal
Summary: Connexins are a family of proteins that play a crucial role in cellular communication, but certain types may have pro-tumorigenic effects. Research has shown that Cx46 can modulate the characteristics of cancer stem cells and epithelial-to-mesenchymal transition in breast cancer cells, potentially influencing future cancer therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Kekoolani S. Visan, Richard J. Lobb, Shu Wen Wen, Justin Bedo, Luize G. Lima, Sophie Krumeich, Carlos Palma, Kaltin Ferguson, Ben Green, Colleen Niland, Nicole Cloonan, Peter T. Simpson, Amy E. McCart Reed, Sarah J. Everitt, Michael P. MacManus, Gunter Hartel, Carlos Salomon, Sunil R. Lakhani, David Fielding, Andreas Moeller
Summary: Lung cancer is a leading cause of cancer-related death worldwide and has low survival rates due to lack of early detection. A study on a lung cancer biomarker based on a simple blood test shows promising advantages for diagnostic assessment. The study identifies a specific micro-RNA, miR-3182, as a potential biomarker for lung cancer diagnosis that can be detected in the blood.
Article
Cell Biology
Miguel V. Guerra, Matias I. Caceres, Andrea Herrera-Soto, Sebastian B. Arredondo, Manuel Varas-Godoy, Brigitte van Zundert, Lorena Varela-Nallar
Summary: This study investigates the role of H3K9 methylation and its methyltransferases Suv39h1 and Suv39h2 in adult hippocampal neurogenesis. The results show that H3K9me3 and H3K9me2 are enriched during early stages of neurogenesis and the expression of Suv39h1 and Suv39h2 is high at these stages. Inhibition of these enzymes reduces neuronal differentiation while increasing proliferation, and knockdown of these enzymes impairs neuronal differentiation in the adult dentate gyrus. These findings highlight the critical involvement of H3K9me3 and its methyltransferases in the regulation of adult hippocampal neurogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Carlos Palma, H. David McIntyre, Carlos Salomon
Summary: Research on extracellular vesicles (EVs) has provided insights into physiological and pathophysiological conditions, particularly during pregnancy. Gestational diabetes mellitus (GDM) is a common and influential complication that affects the miRNAs and proteins within EVs, leading to metabolic changes.
Article
Multidisciplinary Sciences
Songbai Zhang, Ayako Miyakawa, Malin Wickstrom, Cecilia Dyberg, Lauri Louhivuori, Manuel Varas-Godoy, Kati Kemppainen, Shigeaki Kanatani, Dagmara Kaczynska, Ivar Dehnisch Ellstrom, Lotta Elfman, Pauliina Kronqvist, Heli Repo, Katsuhiko Mikoshiba, Cecilia Sahlgren, John Inge Johnsen, Per Uhlen
Summary: This study reveals the negative regulation role of G protein-coupled receptor kinase-interacting protein 1 (GIT1) in the Notch signaling pathway and tumor growth in estrogen receptor-negative (ER-) breast cancer. GIT1 inhibits the translocation of Notch ICD to the nucleus, thus modulating the Notch signaling and acting as a guardian against breast cancer growth.
NATURE COMMUNICATIONS
(2022)
Review
Oncology
Layla Simon, Sofia Sanhueza, Belen Gaete-Ramirez, Manuel Varas-Godoy, Andrew F. G. Quest
Summary: Advances in cancer biology have led to different treatments for improving patients' survival rates. However, relapse and recurrence remain common due to therapy resistance in residual cancer cells, which is influenced by the inflammatory tumor microenvironment (TME) and extracellular vesicles (EVs). Inflammatory conditions alter the release and composition of EVs within the TME, affecting tumor cells' response to cancer therapies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Gabriela Vargas, Omar Cortes, Eloisa Arias-Munoz, Sergio Hernandez, Cristobal Cerda-Troncoso, Laura Hernandez, Alexis E. Gonzalez, Michael H. Tatham, Hianara A. Bustamante, Claudio Retamal, Jorge Cancino, Manuel Varas-Godoy, Ronald T. Hay, Alejandro Rojas-Fernandez, Viviana A. Cavieres, Patricia V. Burgos
Summary: Macroautophagy and the ubiquitin proteasome system are interconnected in maintaining cellular homeostasis. HERPUD1, a protein regulated by proteasomal degradation, stabilizes ER and lysosomal network and negatively regulates autophagy, promoting cell survival under stress conditions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Kimberley Clack, Narshone Soda, Surasak Kasetsirikul, Richard Kline, Carlos Salomon, Muhammad J. A. Shiddiky
Summary: In this study, we developed a novel and rapid method to detect HOTAIR sequences. The method showed high specificity and sensitivity in clinical samples, making it a potential tool for ovarian cancer diagnosis.
Article
Biochemistry & Molecular Biology
Paula Duch, Natalia Diaz-Valdivia, Rafael Ikemori, Marta Gabasa, Evette S. Radisky, Marselina Arshakyan, Sabrina Gea-Sorli, Anna Mateu-Bosch, Paloma Bragado, Josep Lluis Carrasco, Hidetoshi Mori, Josep Ramirez, Cristina Teixido, Noemi Reguart, Cristina Fillat, Derek C. Radisky, Jordi Alcaraz
Summary: This study analyzed the expression of TIMP-1 and its cell surface receptor CD63 in lung adenocarcinoma (ADC) and squamous cell carcinoma (SCC). The researchers found that TIMP-1 was aberrantly overexpressed in ADC-associated fibroblasts and promoted the growth and invasion of ADC cancer cells. The study suggests that the TIMP-1/CD63 interaction could be a potential therapeutic target in lung cancer.
Article
Microbiology
Julia Rubione, Paula S. Perez, Alejandro Czernikier, Gabriel A. Duette, Federico Pehuen Pereyra Gerber, Jimena Salido, Martina P. Fabiano, Yanina Ghiglione, Gabriela Turk, Natalia Laufer, Alejandro J. Cagnoni, Juan M. Perez Saez, Joaquin P. Merlo, Carla Pascuale, Juan C. Stupirski, Omar Sued, Manuel Varas-Godoy, Sharon R. Lewin, Karina Marino, Gabriel A. Rabinovich, Matias Ostrowski
Summary: This study identifies the role of Galectin-1 (Gal-1) in reversing HIV-1 latency and linking inflammation with HIV persistence in cART-treated individuals through activation of the nuclear factor kappa B (NF-kappa B).
Article
Oncology
Natalia Diaz-Valdivia, Layla Simon, Jorge Diaz, Samuel Martinez-Meza, Pamela Contreras, Renato Burgos-Ravanal, Viviana Perez, Balz Frei, Lisette Leyton, Andrew F. G. Quest
Summary: Caveolin-1 (CAV1) plays a dual role in cancer, acting as a tumor suppressor in early stages and promoting metastasis in later stages. CAV1 promotes a metabolic switch to aerobic glycolysis and blocks mitochondrial respiration in cancer cells. This leads to increased reactive oxygen species (ROS) production and enhances the metastatic potential of cancer cells.
Article
Cell Biology
Ignacio Niechi, Jose I. Erices, Diego Carrillo-Beltran, Atenea Uribe-Ojeda, Angelo Torres, Jose Dellis Rocha, Daniel Uribe, Maria A. Toro, Karla Villalobos-Nova, Belen Gaete-Ramirez, Gabriel Mingo, Gareth I. I. Owen, Manuel Varas-Godoy, Lilian Jara, Francisco Aguayo, Veronica A. Burzio, Claudia Quezada-Monras, Julio C. C. Tapia
Summary: Glioblastoma (GBM) is a common and aggressive brain tumor characterized by high recurrence rates. Glioblastoma stem-like cells (GSCs), which are highly resistant to anti-cancer drugs, play a key role in GBM recurrence. This study investigated the role of endothelin-converting enzyme-1 (ECE1) in promoting GSC-like traits in GBM. The researchers found that a phosphorylated variant of ECE1c (ECE1c(K6R)) increased self-renewal and stem-like characteristics in GBM cells, as well as enhanced resistance to chemotherapy drugs and invasiveness. These findings suggest that phosphorylated ECE1c may serve as a prognostic marker and potential therapeutic target for GBM.
Review
Biochemistry & Molecular Biology
Francisca Sepulveda, Cristina Mayorga-Lobos, Kevin Guzman, Eduardo Duran-Jara, Lorena Lobos-Gonzalez
Summary: Cancer research identifies the tumor microenvironment (TME) as a crucial area of investigation, with a focus on understanding the communication between tumor cells and various cell types within the TME. Exosomes, as important mediators of cell-to-cell communication, transfer molecular cargo, including miRNAs, which play critical roles in intercellular communication. Comprehensive studies investigating the involvement of exosomes and their miRNA cargo in the TME are necessary for a deeper understanding of these complex interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Johan Macuer-Guzman, Claudia Giovagnoli-Vicuna, Giuliano Bernal, Lorena Lobos-Gonzalez, Erwin de la Fuente-ortega, Michael Araya-Castillo, Cristian Ibanez
Summary: This study evaluated the antiproliferative and apoptotic effects of an ethanol macerate extract from Annona cherimola seed (EMCHS) in human gastric cancer cells. EMCHS reduced the tumor cell cloning capacity and induced apoptosis. UHPLC-MS/MS analysis identified two acetogenins as potential key components responsible for the selective antiproliferative effect of EMCHS. In addition, GC-FID analysis detected the presence of fatty acids, including oleic acid, which has known antitumor activity. Thus, EMCHS shows potential as a natural therapy for gastric cancer.
Article
Biology
T. Koning, F. Cordova, G. Aguilar, J. Sarmiento, G. A. Mardones, M. Boric, M. Varas-Godoy, A. Lladser, W. N. Duran, P. Ehrenfeld, F. A. Sanchez
Summary: Nitric oxide (NO) is produced by different isoforms of nitric oxide synthases and activates two signaling pathways, one dependent on soluble guanylate cyclase and protein kinase G, and the other involving post-translational modification of proteins through S-nitrosylation. High levels of NO have been detected in blood of breast cancer patients, suggesting a positive correlation between NO biosynthesis, malignancy, and metastasis. The study shows that S-nitrosylation in the endothelium enhances VCAM-1 surface localization, promoting leukocyte and tumor cell adhesion and extravasation, potentially contributing to breast cancer metastasis.
BIOLOGICAL RESEARCH
(2023)