Review
Immunology
Linde Dekker, Evy Sanders, Caroline A. Lindemans, Coco de Koning, Stefan Nierkens
Summary: The recovery of naive T cells plays an important role in allo-HCT by functioning as both drivers and regulators in allogeneic reactions. Adequate reconstitution of naive T cells can improve GvHD prevention while maintaining GvL effects to lower relapse risk.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Ying Fang, Yichen Zhu, Adam Kramer, Yuning Chen, Yan-Ruide Li, Lili Yang
Summary: Allogeneic cell therapies have the potential to be a cost-effective solution for cancer immunotherapy, but the development of graft-versus-host disease (GvHD) remains a challenge. Innate T cells such as MAIT cells, iNKT cells, and gamma delta T cells offer a promising solution as they express MHC-independent T-cell receptors (TCRs) and can avoid MHC recognition and GvHD. This review examines their biology, roles in GvHD modulation and allo HSCT, and explores their potential futures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Hua Jiang, Denggang Fu, Alan Bidgoli, Sophie Paczesny
Summary: Allogeneic hematopoietic cell transplantation (allo-HCT) is crucial for treating hematological malignancies, but acute and chronic graft-versus-host disease (aGVHD and cGVHD) are major complications. Understanding the roles of T cell subsets in GVHD pathogenesis is essential for identifying new therapeutic targets and improving outcomes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yiwen Liang, Jingyi Shen, Qiu Lan, Kexin Zhang, Yan Xu, Maxwell Duah, Kailin Xu, Bin Pan
Summary: This research found that PD-1 expression on T cells increased in mice with chronic graft-versus-host disease (cGVHD). Intervention of the PD-1/PD-L1 pathway showed a significant impact on the occurrence of cGVHD and graft-versus-tumor effect.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Immunology
Ying Huang, Yujing Zou, Yiqun Jiao, Peijie Shi, Xiaoli Nie, Wei Huang, Chuanfeng Xiong, Michael Choi, Charles Huang, Andrew N. Macintyre, Amanda Nichols, Fang Li, Chuan-Yuan Li, Nancie J. MacIver, Diana M. Cardona, Todd Brennan, Zhiguo Li, Nelson J. Chao, Jeffrey C. Rathmell, Benny J. Chen
Summary: The study demonstrates the critical role of glycolysis in inducing graft-versus-host disease (GVHD) and suggests that targeting glycolysis selectively prevents GVHD while preserving non-alloreactive T cells that protect against tumor growth. The findings provide important insights into the metabolic reprogramming of donor T cells and offer a potential therapeutic approach for improving the clinical outcome of transplantation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Saurabh Kumar Gupta, Dievya Gohil, Deepshikha Dutta, Girish Ch Panigrahi, Puja Gupta, Kajal Dalvi, Twinkle Khanka, Subhash Yadav, Rajiv Kumar Kaushal, Akanksha Chichra, Sachin Punatar, Anant Gokarn, Sumeet Mirgh, Nishant Jindal, Lingaraj Nayak, Prasanth R. Tembhare, Syed Khizer Hasan, Santosh Kumar Sandur, Lal Hingorani, Navin Khattry, Vikram Gota
Summary: Researchers have found that Withaferin-A (WA), a phytomolecule obtained from Withania somnifera, has significant efficacy in preventing and treating acute graft versus host disease (aGvHD), which accounts for a significant proportion of non-relapse mortality and morbidity in patients undergoing allogeneic hematopoietic stem cell transplantation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Ning Wu, Ruoyang Liu, Shuang Liang, Haitao Gao, Lan-Ping Xu, Xiao-Hui Zhang, Jiangying Liu, Xiao-Jun Huang
Summary: In the development of aGVHD, gamma delta T cells were found to indirectly regulate the migration of CD4 T cells to target tissues, rather than directly triggering alloreactive responses. This study suggests a potential role for gamma delta T cells in recruiting alloreactive CD4 T cells through the expression of CXCR4, providing new insights into the mechanism of aGVHD and potential therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Nona Janikashvili, Claire Gerard, Marine Thebault, Andrea Brazdova, Clovis Boibessot, Claudie Cladiere, Marion Ciudad, Helene Greigert, Sethi Ouandji, Thibault Ghesquiere, Maxime Samson, Sylvain Audia, Philippe Saas, Bernard Bonnotte
Summary: HuMoSC-based therapy is a promising approach for controlling GvHD by preserving their suppressive function in inflammatory conditions and in the presence of immunosuppressive agents. Clinical experiments have shown that the combination of HuMoSC with immunosuppressants effectively controls GvHD with a synergistic effect.
Article
Surgery
Gregory Ehx, Caroline Ritacco, Muriel Hannon, Sophie Dubois, Loic Delens, Evelyne Willems, Sophie Servais, Pierre Drion, Yves Beguin, Frederic Baron
Summary: RAPA mitigates GVHD by decreasing T cell engraftment and differentiation, inhibiting CD8(+) T cell activation and increasing long-term IL-2 secretion to support Treg proliferation, while maintaining graft-versus-leukemia effects. The impact of RAPA on Treg and CD8(+) T cells is dependent on IL-2 signaling, with therapeutic options interfering with IL-2 antagonizing the promotion of Treg by RAPA. Synergistic effects may be achieved by combining RAPA with drugs like 5-azacytidine.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Polymer Science
Wafa Al-Gethami, Noha Al-Qasmi
Summary: This study aimed to prepare antimicrobial nanocomposites consisting of alginate, chitosan, and camptothecin. The results showed a strong chemical interaction between alginate and chitosan, and successful loading of camptothecin in both Ca-Alg(2)/CPT and Ca-Alg(2)-CH/CPT nanomaterials. Antimicrobial tests revealed that Ca-Alg(2)-CH/CPT nanocomposite was more effective against Escherichia coli and Klebsiella pneumoniae, while Ca-Alg(2)/CPT was less efficient in killing all three types of bacteria. PCR analysis indicated changes in bacterial genetic material due to the nanomaterial processing.
Review
Hematology
Khaled Sanber, Bipin Savani, Tania Jain
Summary: CAR T-cell therapy has revolutionized the treatment of hematologic malignancies, but also introduced potential toxicities, including the risk of graft-versus-host disease (GVHD) in certain settings. Understanding and managing this risk is crucial for the safe development and use of CAR T-cell therapies in clinical practice.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Review
Immunology
Fathima A. Mohamed, Govindarajan Thangavelu, Stephanie Y. Rhee, Peter T. Sage, Roddy S. O'Connor, Jeffrey C. Rathmell, Bruce R. Blazar
Summary: The therapeutic efficacy of allo-HSCT is limited by the development of GVHD, with immune dysregulation leading to expansion of alloreactive cells and reduction in regulatory cells. New therapies are needed to restore the balance between effector and regulatory immune cells while preserving the beneficial graft-versus-tumor effect.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yoshiyuki Tago, Chiho Kobayashi, Mineko Ogura, Jutaro Wada, Sho Yamaguchi, Takashi Yamaguchi, Masahiro Hayashi, Tomoyuki Nakaishi, Hiroshi Kubo, Yasuyoshi Ueda
Summary: The study found that human amnion-derived mesenchymal stem cells (AMSC) exhibit significant immunosuppressive effects and can delay the progression of acute graft-versus-host disease (GVHD) mainly by preventing T cell activation and proliferation through the PD-1 pathway.
SCIENTIFIC REPORTS
(2021)
Article
Oncology
Marie Bleakley, Alison Sehgal, Stuart Seropian, Melinda A. Biernacki, Elizabeth F. Krakow, Ann Dahlberg, Heather Persinger, Barbara Hilzinger, Paul J. Martin, Paul A. Carpenter, Mary E. Flowers, Jenna Voutsinas, Theodore A. Gooley, Keith Loeb, Brent L. Wood, Shelly Heimfeld, Stanley R. Riddell, Warren D. Shlomchik
Summary: This study evaluated the impact of T-N depletion on chronic GVHD (cGVHD) and other outcomes. The results showed that T-N depletion significantly reduced the incidence of GVHD without increasing the risk of relapse and nonrelapse mortality.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Misa Noguchi, Takero Shindo, Yosuke Yamada, Hiroshi Date
Summary: This case report describes a 59-year-old woman with dermatomyositis-associated interstitial pneumonia who developed GVHD following a lung transplant. The patient's diagnosis and treatment process of GVHD are outlined in the report.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2021)
Article
Engineering, Biomedical
Guang Yang, Bhushan Mahadik, Ji Young Choi, Justine R. Yu, Trevor Mollot, Bin Jiang, Xiaoming He, John P. Fisher
Summary: The study utilized a microfluidic approach to prepare collagen microbeads loaded with human bone marrow derived mesenchymal stem cells for creating engineered bone grafts. The cell-laden microbeads self-assembled into macro-tissues, enabling precise control over cell spatial distribution at the macroscale, offering flexibility for graft volume and architecture. The approach has the potential to create 3D engineered bone grafts with customized vasculature using modular tissue engineering and 3D printing.
ACTA BIOMATERIALIA
(2021)
Article
Multidisciplinary Sciences
Hai Wang, Yutong Liang, Yue Yin, Jie Zhang, Wen Su, Alisa M. White, Bin Jiang, Jiangsheng Xu, Yuntian Zhang, Samantha Stewart, Xiongbin Lu, Xiaoming He
Summary: The FSCNO hybrid nanoparticle targets tumor vasculature to release P-gp inhibitor and anticancer drug, improving drug bioavailability inside cells. The nano-onion shows superior light absorption in the NIR region, enabling triggered drug release at low power.
NATURE COMMUNICATIONS
(2021)
Article
Biophysics
Hao Chen, Bin Jiang, James G. Shamul, Xiaoming He
Summary: The beating pattern of 3D iPSC-CSs can be conveniently detected and quantified by calculating the relative change of entropy in all frames/images of non-fluorescent optical signal without labeling any cells. This label-free method enables rapid detection and quantification of the beating pattern of each iPSC-CS, as well as reliable detection of their responses to anticancer and cardiac drugs.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Anatomy & Morphology
Jenna Dumbleton, James G. Shamul, Bin Jiang, Pranay Agarwal, Haishui Huang, Xiaofeng Jia, Xiaoming He
Summary: This study investigates directed neural differentiation of murine ESCs using a non-planar microfluidic device to encapsulate cells in 3D microcapsules with alginate hydrogel shells. Modifications of the hydrogel shell facilitate early neurite extension and demonstrate potential for further neural differentiation into specialized neurons for therapeutic applications.
CELLS TISSUES ORGANS
(2022)
Article
Engineering, Biomedical
Bin Jiang, Wenquan Ou, James G. Shamul, Hao Chen, Sarah Van Belleghem, Samantha Stewart, Zhenguo Liu, John P. Fisher, Xiaoming He
Summary: The use of Rock inhibitor (RI) in 3D cardiac differentiation of human induced pluripotent stem cells (iPSCs) poses challenges, but reducing the RI concentration can improve efficiency and homogeneity, which is important for understanding and treating heart diseases.
BIOACTIVE MATERIALS
(2022)
Article
Thermodynamics
Elyahb Allie Kwizera, Samantha Stewart, Md Musavvir Mahmud, Xiaoming He
Summary: Magnetic nanoparticles, especially superparamagnetic iron oxide nanoparticles, have garnered significant attention for their easy synthesis, functionalization, and control over size and shape. They have shown potential in various biomedical applications, including thermal treatments for cancer and enhancement of cryopreservation. Challenges in the use of magnetic nanoparticles in these biomedical applications are also discussed.
JOURNAL OF HEAT TRANSFER-TRANSACTIONS OF THE ASME
(2022)
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, Yunhua Liu, Sheng Liu, Wenquan Ou, Alisa White, Samantha Stewart, Katherine H. R. Tkaczuk, Lee M. Ellis, Jun Wan, Xiongbin Lu, Xiaoming He
Summary: Colon and rectal cancers are leading causes of cancer-related deaths in the United States. Genomic analyses reveal the frequent hemizygous deletion of TP53 gene, an important tumor suppressor, in both cancers. Targeting TP53 directly has been unsuccessful, but the co-deletion of POLR2A gene with TP53 in these cancers suggests RNA interference with POLR2A siRNA as a potential strategy. However, efficient delivery of siRNA into the cytosol is a major challenge. To overcome this barrier, pH-responsive MetC-nanoparticles with a bomb effect are developed for effective cytosolic delivery of POLR2A siRNA, enhancing therapeutic efficacy for TP53-deficient cancer.
Article
Chemistry, Multidisciplinary
Jiangsheng Xu, James G. Shamul, Elyahb Allie Kwizera, Xiaoming He
Summary: Mitochondria are critical organelles for producing energy in cells and can be targeted for cancer therapy. Mitochondria-targeting nanotechnologies have shown high efficacy for cancer treatment both in vitro and in vivo, and can be intelligently designed based on the characteristics of the tumor microenvironment.
Article
Chemistry, Multidisciplinary
Elyahb A. Kwizera, Wenquan Ou, Sojeong Lee, Samantha Stewart, James G. Shamul, Jiangsheng Xu, Nancy Tait, Katherine H. R. Tkaczuk, Xiaoming He
Summary: In this study, a robust electromicrofluidic chip was developed for efficient capture and release of heterogeneous circulating tumor cells (CTCs), which is important for cancer research and therapeutic development.
Correction
Chemistry, Multidisciplinary
Elyahb A. Kwizera, Wenquan Ou, Sojeong Lee, Samantha Stewart, James G. Shamul, Jiangsheng Xu, Nancy Tait, Katherine H. R. Tkaczuk, Xiaoming He
Article
Engineering, Biomedical
Bin Jiang, Alisa White, Wenquan Ou, Sarah Van Belleghem, Samantha Stewart, James G. Shamul, Shaik O. Rahaman, John P. Fisher, Xiaoming He
Summary: The conventional approach for fabricating PDMS microfluidic devices is inconvenient and requires specialized facilities. This study discovered that reducing the curing agent for crosslinking the PDMS prepolymer increases the noncovalent binding energy of the PDMS surfaces, allowing for the convenient fabrication of leak-free microfluidic devices without the need for plasma or other treatments. This method enables easy loading and retrieval of cells and is faster and more convenient than the conventional covalent bonding method.
BIOACTIVE MATERIALS
(2022)
Article
Genetics & Heredity
Pei-Chih Lee, Samantha Stewart, Olga Amelkina, Hannah Sylvester, Xiaoming He, Pierre Comizzoli
Summary: Intracellular trehalose delivery through CRNPs improves the dehydration tolerance of COCs, opening up new possibilities for oocyte storage and preservation of fertility at ambient temperatures.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Retraction
Chemistry, Multidisciplinary
Hai Wang, Pranay Agarwal, Gang Zhao, Guang Ji, Christopher M. Jewell, John P. Fisher, Xiongbin Lu, Xiaoming He
ACS CENTRAL SCIENCE
(2023)
Article
Engineering, Biomedical
Samantha Stewart, Wenquan Ou, Helim Aranda-Espinoza, Shaik O. Rahaman, Xiaoming He
Summary: Ovarian follicles develop in a regulated mechanical microenvironment, and disruptions to this microenvironment can cause infertility. The viscoelastic properties of ovarian tissue have not been extensively studied. This study characterizes the elastic and viscoelastic properties of ovarian tissue from both reproductive age groups of domestic cats and identifies possible connections between mechanical and compositional heterogeneities. These findings may have important implications for biomimetic follicle culture.
ACTA BIOMATERIALIA
(2023)
Review
Reproductive Biology
Pierre Comizzoli, Xiaoming He, Pei-Chih Lee
Summary: Preserving germ cells and gonadal tissues for the long term is critical in fertility treatment and animal population management. Current options at freezing temperatures are often constraining, but recent research suggests alternative strategies based on dehydration and storage at supra-zero temperatures. Further studies are needed for rehydration and reanimation of germ cells.
REPRODUCTION AND FERTILITY
(2022)