期刊
MOLECULES
卷 23, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/molecules23113007
关键词
click chemistry; nucleoside and nucleotide analogues; indirect immunocytochemistry; isotopes
资金
- Ministry of Education, Youth and Sports [LO1304]
- Czech Health Research Council [15-31604A]
- European Regional Development Fund-Project ENOCH [CZ.02.1.01/0.0/0.0/16_019/0000868]
The replication of nuclear and mitochondrial DNA are basic processes assuring the doubling of the genetic information of eukaryotic cells. In research of the basic principles of DNA replication, and also in the studies focused on the cell cycle, an important role is played by artificially-prepared nucleoside and nucleotide analogues that serve as markers of newly synthesized DNA. These analogues are incorporated into the DNA during DNA replication, and are subsequently visualized. Several methods are used for their detection, including the highly popular click chemistry. This review aims to provide the readers with basic information about the various possibilities of the detection of replication activity using nucleoside and nucleotide analogues, and to show the strengths and weaknesses of those different detection systems, including click chemistry for microscopic studies.
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