期刊
MOLECULES
卷 23, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/molecules23102653
关键词
cyanobacteria; cyclic lipopeptides (CLPs); muscotoxins; high performance countercurrent chromatography (HPCCC); antimicrobial activity; gamma-methylproline
资金
- GA CR grant [16-09381S]
- Ministry of Education, Youth and Sports of the Czech Republic [LO1416]
- ALGAMIC project [CZ.1.05/2.1.00/19.0392]
- COST Action [FA1206]
- Ministry of Agriculture of Czech Republic [RO1018]
- Cross-Border cooperation Czech-Bavaria-Project [41]
- [LD14101]
Muscotoxins are cyanobacterial cyclic lipopeptides with potential applications in biomedicine and biotechnology. In this study, Desmonostoc muscorum CCALA125 strain extracts were enriched by polymeric resin treatment, and subjected to HPCCC affording three cyclic lipopeptides (1-3), which were further repurified by semi-preparative HPLC, affording 1, 2, and 3, with a purity of 86%, 92%, and 90%, respectively. The chemical identities of 2-3 were determined as muscotoxins A and B, respectively, by comparison with previously reported ESI-HRMS/MS data, whereas 1 was determined as a novel muscotoxin variant (muscotoxin C) using NMR and ESI-HRMS/MS data. Owing to the high yield (50 mg), compound 2 was broadly screened for its antimicrobial potential exhibiting a strong antifungal activity against Alternaria alternata, Monographella cucumerina, and Aspergillus fumigatus, with minimum inhibitory concentration (MIC) values of 0.58, 2.34, and 2.34 mu g/mL; respectively, and weak antibacterial activity against Bacillus subtilis with a MIC value of 37.5 mu g/mL. Compounds 1 and 3 were tested only against the plant pathogenic fungus Sclerotinia sclerotiorum due to their low yield, displaying a moderate antifungal activity. The developed chromatographic method proved to be an efficient tool for obtaining muscotoxins with potent antifungal properties.
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