Article
Biochemistry & Molecular Biology
Diego Rey-Varela, Miguel Balado, Manuel L. Lemos
Summary: Aeromonas salmonicida subsp. salmonicida (A. salmonicida), a Gram-negative bacterium causing furunculosis in fish, produces siderophores acinetobactin and amonabactins to extract iron, but the regulation pathways and conditions for their production are not clear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Pierre-Etienne Marcoux, Sarah B. Girard, Kim C. Fournier, Catherine A. Tardif, Ariane Gosselin, Steve J. Charette
Summary: This study reveals the fusion process between the pAsa5 plasmid and the complete version of the pAsa8 plasmid. The insertion of the pAsa8 transposon into pAsa5 in a certain strain appears to be an abnormal event compared to the fusion of the two full-length plasmids. The fusion is mediated by a 22-nucleotide sequence present in both plasmids. Furthermore, the study shows that pAsa8 can be introduced into new strains through conjugation and fusion with pAsa5 can be detected.
Article
Virology
Nava Hosseini, Valerie E. Paquet, Mahdi Chehreghani, Sylvain Moineau, Steve J. Charette
Summary: Aquaculture is facing antibiotic resistance issues in fighting pathogenic bacteria, with phage therapy being a potential alternative. A specific combination of four phages has shown effectiveness against A. salmonicida subsp. salmonicida, but strains with Prophage 3 exhibit reduced sensitivity. This highlights the impact of prophage content on the efficacy of phage therapy.
Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Infectious Diseases
Kim C. Fournier, Valerie E. Paquet, Sabrina A. Attere, Judith Farley, Helene Marquis, Hubert Gantelet, Christian Ravaille, Antony T. Vincent, Steve J. Charette
Summary: New variants of the pRAS3 plasmid were discovered, along with a genetic element similar to one found in a swine pathogen. Despite differences between plasmids, no specific trend in tetracycline resistance was observed. Furthermore, Blast analysis revealed the widespread distribution of pRAS3 plasmids in other bacterial species.
Article
Fisheries
Emmanuelle Moreau, Lionel Pineau, Florine Bachelet, Antoine Rostang, Kenny Oberle, Segolene Calvez
Summary: This study aimed to develop a method of infectious challenge in large Rainbow trout by immersion, simulating natural infection conditions. The results showed that a 24-hour contact period led to infection in all fish with a mortality rate of 53.25%. The infected fish developed acute infection symptoms and lesions similar to furunculosis, and antibodies against the bacterium were produced 4 weeks after challenging compared to the non-challenged group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Infectious Diseases
Pierre-Etienne Marcoux, Sabrina A. Attere, Valerie E. Paquet, Maude F. Paquet, Sarah B. Girard, Judith Farley, Michel Frenette, Antony T. Vincent, Steve J. Charette
Summary: We discovered a plasmid in Aeromonas salmonicida subsp. salmonicida, called pAsa-2939, which carries antibiotic resistance genes. This 67 kb plasmid displays unique features and contains a transposon with an unusual organization. The transposon carries a catB3 gene that has not been found in this bacterial species before. The plasmid can be transferred into Aeromonas hydrophila, but not into Escherichia coli.
Article
Fisheries
Sarah B. Girard, Valerie E. Paquet, Steve J. Charette
Summary: Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A-layer, lipases and proteases during infection. This study optimized protocols for liquid tests to quickly and accurately determine the presence of these virulence factors. The new protocols provide opportunities for quicker characterization and more precise results for A. salmonicida subsp. salmonicida strains.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Qiang Fu, Hao Zhang, Yuqing Li, Pei Zhang, Chengbin Gao, Jie Li, Xingchun Li, Min Cao, Chao Li
Summary: This study conducted transcriptome analysis in Atlantic salmon head kidney following the administration of ASM vaccination, revealing genes, their expression patterns, and pathways involved in immune responses. The results showed significant up-regulation and down-regulation of genes post vaccination, with many differentially expressed genes involved in cell adhesion, cytokine-cytokine receptor interaction, viral infection, and signaling pathway related to immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Tobias Kroniger, Mina Mehanny, Rabea Schluter, Anke Trautwein-Schult, Bernd Kollner, Dorte Becher
Summary: We analyzed the proteomic response of the fish pathogen A. salmonicida to iron limitation, elevated incubation temperature, and antibiotic treatment. Several iron-regulated proteins were identified, some of which were not previously reported. We also observed increased abundance of certain proteins under both iron limitation and elevated temperature, suggesting their involvement in the infection process. Analysis of outer membrane vesicles revealed significant differences in proteomes, indicating their potential role in iron acquisition. The presence of iron-regulated transporters suggests that OMVs from stressed bacteria could be suitable vaccine candidates for inducing anti-virulence immune responses.
Article
Environmental Sciences
Jeff Gauthier, Helene Marquis, Valerie E. Paquet, Steve J. Charette, Roger C. Levesque, Nicolas Derome
Summary: Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium causing furunculosis in fish, but in vivo pathogenicity data is currently lacking. Using a standardized furunculosis challenge model with a prototype strain like A. salmonicida subsp. salmonicida 890054 can provide valuable information, and computational analysis of the genome can reveal important insights.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
Yongwei Yan, Yaokuan Liu, Zhaolan Mo, Jie Li, Shulan Liu, Ye Gao, Guiyang Li, Jie Li
Summary: A developed A. salmonicida subsp. masoucida inactivated vaccine for turbot has been proven to be effective in protecting the fish for at least 24 weeks under both laboratory and field conditions, with the emulsified vaccine showing better results than the water based vaccine. The vaccine not only reduces the infection probability by other pathogens or parasites, but also promotes fish growth without severe side-effects.
Article
Fisheries
Yibin Yang, Tao Mao, Bin Xu, Xia Zhu, Lin Zhang, Ying Huang, Hongyu Zhang, Jin Xu, Xiaohui Ai
Summary: This study investigated the characteristics of a dominant strain wx3 isolated from sick red swamp crayfish and analyzed the immune response of the crayfish under stress. The whole genome analysis confirmed that wx3 is the pathogen Aeromonas salmonicida subsp. Masoucida, which causes red swamp crayfish head rot. The study also found that wx3 is highly sensitive to certain antibiotics and that infected crayfish show a strong immune response.
Article
Fisheries
Asmaa K. Al-Mokaddem, Dalia A. Abdel-moneam, Reham A. Ibrahim, Mona Saleh, Mohamed Shaalan
Summary: Aeromonas salmonicida was isolated for the first time from Mugil carinatus fish in Suez Gulf, Egypt. The bacteriological, molecular and histopathological methods were used to identify the fish samples. The isolated A. salmonicida strains were confirmed through biochemical identification, molecular identification and histopathological analysis. The study also revealed the pathological lesions caused by A. salmonicida infection.
AQUACULTURE REPORTS
(2022)
Article
Infectious Diseases
Manuel Soto-Davila, Setu Chakraborty, Javier Santander
Summary: This study evaluated the gene expression of A. salmonicida in fish infection studies and identified multiple reliable reference genes. The use of multiple reference genes and validation helps to minimize variability in qPCR studies.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Biochemistry & Molecular Biology
Dawrin Pech-Puch, Diana Grilo, Susana Eunice Calva-Perez, Andreia Pedras, Harold Villegas-Hernandez, Sergio Guillen-Hernandez, Raul Diaz-Gamboa, Mateo Forero Tunjano, Jaime Rodriguez, Oscar A. Lenis-Rojas, Carlos Jimenez, Catarina Pimentel
Summary: This study screened 65 extracts from marine organisms of the Yucatan Peninsula, Mexico, for antifungal activity and identified nine crude extracts with potent antifungal activity, including four from sponge species. The chemical composition analysis of one of the most active fractions revealed the presence of mirabilin B and penaresidin B. These findings highlight the marine organisms of the Yucatan Peninsula as important sources of natural products with promising fungicidal activity, which could greatly advance the treatment of invasive fungal infections, particularly in immunosuppressed patients.
Article
Chemistry, Inorganic & Nuclear
M. Carmen de la Fuente, Lucia Ageitos, Marta A. Lages, Diana Martinez-Matamoros, Abel M. Forero, Miguel Balado, Manuel L. Lemos, Jaime Rodriguez, Carlos Jimenez
Summary: This study successfully performed stereo-selective total synthesis of Pcb analogues, which are siderophores produced by pathogenic Gram-negative bacteria. The replacement of the acid-sensitive α-methylthiazoline moiety with a more stable thiazole ring and varying the OH group configuration at C-13 resulted in different metal coordination abilities. The study also confirmed the stereochemistry of the Ga3+ complex and proposed the scaffold's potential use in designing new chelating agents or antibacterials.
INORGANIC CHEMISTRY
(2023)
Review
Chemistry, Medicinal
Susana P. Gaudencio, Engin Bayram, Lada Lukic Bilela, Mercedes Cueto, Ana R. Diaz-Marrero, Berat Z. Haznedaroglu, Carlos Jimenez, Manolis Mandalakis, Florbela Pereira, Fernando Reyes, Deniz Tasdemir
Summary: Natural Products (NP) play a crucial role in the discovery of novel drugs and biotechnological applications. However, the process of NP discovery is expensive and time-consuming, mainly due to the challenges of dereplication and structure elucidation, especially for metabolites with stereogenic centers. This review highlights recent technological and instrumental advances that address these obstacles and accelerate NP discovery for biotechnological applications. It emphasizes innovative high-throughput tools and methods for bioactivity screening, NP chemical analysis, dereplication, metabolite profiling, metabolomics, genome sequencing and/or genomics approaches, databases, bioinformatics, chemoinformatics, and three-dimensional NP structure elucidation.
Article
Microbiology
Alba V. Barca, Ana Vences, Mateus S. Terceti, Ana do Vale, Carlos R. Osorio
Summary: This study investigated the transcriptional response of the facultative marine pathogen Photobacterium damselae subsp. damselae to changes in salinity. It was found that low salinity conditions triggered a virulence gene expression profile, while growth at high salinity upregulated genes involved in energy production and metabolism. This study enhances our understanding of the salinity-responsive adaptations of a generalist marine pathogen.
Article
Food Science & Technology
Juan Carlos Fuentes-Monteverde, Marvin J. Nunez, Oscar Amaya-Monterosa, Morena L. Martinez, Jaime Rodriguez, Carlos Jimenez
Summary: This study presents a multistage methodology for detecting small amounts of tetrodotoxin in fish and potentially other marine organisms. The methodology was applied to porcupinefish specimens collected in El Salvador. It involved a three-stage approach, including sample screening, high-resolution mass spectrometry analyses, and further analysis using different techniques. This study reports the presence of tetrodotoxin in D. hystrix collected in El Salvador for the first time.
Article
Microbiology
Marta A. Lages, Ana do Vale, Manuel L. Lemos, Miguel Balado
Summary: This study found that PbtA not only interacts with irp-HPI promoters but also regulates the expression of many genes in V. anguillarum, including genes involved in metabolism, immunity, and virulence. These results are significant for understanding the regulatory mechanisms of irp-HPI island and its pathogenicity.
Article
Biotechnology & Applied Microbiology
Oumayma Lafi, Rym Essid, Laurence Lachaud, Carlos Jimenez, Jaime Rodriguez, Lucia Ageitos, Ridha Mhamdi, Leila Abaza
Summary: This study assessed the antileishmanial activity of biomolecules obtained from Olea europaea L. leaves and twigs from different Tunisian cultivars. The cyclohexane fraction of cv. Chemlali showed the strongest leishmanicidal activity against all tested leishmanial strains. Three triterpenic compounds were identified, including oleanolic acid, erythrodiol, and uvaol, which demonstrated antileishmanial activity. The mixture of these compounds exhibited enhanced activity against Leishmania tropica compared to individual compounds.
Article
Immunology
A. Rodriguez-Pedrouzo, J. Cisneros-Sureda, D. Martinez-Matamoros, D. Rey-Varela, M. Balado, J. Rodriguez, M. L. Lemos, M. Folgueira, C. Jimenez
Summary: Zebrafish is an excellent model for studying bacterial infections in fish. This study used zebrafish as a model to investigate the infection process of Aeromonas salmonicida in fish and evaluated the use of fluorescence microscopy to track the infection in vivo.
MICROBIAL PATHOGENESIS
(2023)