期刊
MOLECULAR BIOTECHNOLOGY
卷 61, 期 1, 页码 60-71出版社
HUMANA PRESS INC
DOI: 10.1007/s12033-018-0133-0
关键词
Ribosome display; Display technologies; Affinity maturation; Stability optimization; Evolutionary strategies; Diagnostics; Therapeutics
资金
- National Natural Science Foundation of China [31470967]
Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. This tool has been successfully exploited for the selection of functional and specific binders in vitro. This review provides a comprehensive survey of the applications of ribosome display in screening or evolving functional proteins as well as in diagnostics and therapeutics. Previous papers on ribosome display failed to comprehensively review evolutionary strategies for proteins. In the present paper, we review all existing evolutionary strategies that have been combined with ribosome display. We also discuss shortcomings, improvement strategies, and research tendency.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据