Review
Biotechnology & Applied Microbiology
Gwang-Bum Im, Ruei-Zeng Lin
Summary: Gelatin methacrylate (GelMA) hydrogels have excellent biocompatibility and biodegradability, making them widely used in tissue engineering and regenerative medicine. The mechanical characteristics of GelMA hydrogels can be adjusted by changing crosslinking conditions, allowing them to closely resemble the native extracellular matrix (ECM) properties. GelMA hydrogels have applications in bioengineering human vascular networks, both in vitro and in vivo. They can be used for disease modeling, drug screening, and ensuring oxygenation of tissue-engineered constructs. GelMA hydrogels can also be used as implantable materials for delivering therapeutic cells to rebuild vasculature in ischemic wounds.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Fengyi Liu, Xu Wang, Yuzhou Li, Mingxing Ren, Ping He, Lu Wang, Jie Xu, Sheng Yang, Ping Ji
Summary: In this study, a photocrosslinked GelMA/PAMAM-MA hydrogel was developed as a stem cell carrier for cartilage repair. The hybrid hydrogel showed improved structural and mechanical properties compared to GelMA alone, and demonstrated a sufficient ability to promote cartilage regeneration in both in vitro and in vivo experiments. This study provides new insight into the design strategy of stem cell delivery scaffolds.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Materials Science, Multidisciplinary
Chun Liu, Ting Dai, Xiaoyu Wu, Jiayi Ma, Jun Liu, Siyu Wu, Lei Yang, Hongbin Zhao
Summary: This study investigated the potential of nano-attapulgite (nano-ATP)/GelMA composite hydrogels for bone tissue engineering. The incorporation of nano-ATP improved the printability and mechanical properties of the hydrogels, resulting in enhanced bone regeneration and angiogenesis. This research highlights the clinical practicality of nano-ATP for bone defect repair.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Louis J. Born, Shannon T. McLoughlin, Dipankar Dutta, Bhushan Mahadik, Xiaofeng Jia, John P. Fisher, Steven M. Jay
Summary: This study demonstrates the incorporation of extracellular vesicles (EVs) from mesenchymal stem/stromal cells (MSCs) into 3D-printed gelatin methacrylate (GelMA) hydrogel bioink. The initial burst release of EVs can be reduced by increasing the concentration of crosslinker during gelation. The bioactivity of MSC EVs is retained after the 3D printing and photocrosslinking processes, indicating the therapeutic potential of MSC EV-laden GelMA bioinks.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biotechnology & Applied Microbiology
Ana L. Alves, Ana C. Carvalho, Ines Machado, Gabriela S. Diogo, Emanuel M. Fernandes, Vania I. B. Castro, Ricardo A. Pires, Jose A. Vazquez, Ricardo I. Perez-Martin, Miguel Alaminos, Rui L. Reis, Tiago H. Silva
Summary: This study evaluated the potential of Gelatin Methacryloyl (GelMA) hydrogels produced from marine-derived gelatin for corneal stromal applications. The hydrogels exhibited excellent optical and swelling properties, easy handling, structural stability, and adequate degradation rates. The addition of ascorbic acid enhanced the biological performance of the cells and induced collagen production.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Ryoma Goto, Eisaku Nishida, Shuichiro Kobayashi, Makoto Aino, Tasuku Ohno, Yuki Iwamura, Takeshi Kikuchi, Jun-ichiro Hayashi, Genta Yamamoto, Masaki Asakura, Akio Mitani
Summary: The study found that visible-light-crosslinked GelMA hydrogels, along with encapsulated osteoblasts, exhibited good mechanical and biological properties, making them suitable for use as scaffolds for bone regeneration. Osteoblasts encapsulated in GelMA hydrogels photopolymerized with visible light showed higher cell viability. Additionally, GelMA-RF hydrogels promoted osteoblast differentiation and have the potential to treat bone-destructive diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Jimmy Jiun-Ming Su, Chih-Hsin Lin, Hsuan Chen, Shyh-Yuan Lee, Yuan-Min Lin
Summary: In this study, a cell-laden GelMA composite hydrogel incorporating silanized hydroxyapatite (Si-HAp) and a visible light crosslinking system was developed to enhance mechanical properties and promote osteogenic gene expression in cells. The incorporation of Si-HAp also increased resolution and effectiveness in bone regeneration, making the GelMA-Si-HAp composite hydrogel an effective biomaterial for bone tissue engineering applications.
Article
Engineering, Biomedical
Liu Qu, Nileshkumar Dubey, Juliana S. Ribeiro, Ester A. F. Bordini, Jessica A. Ferreira, Jinping Xu, Rogerio M. Castilho, Marco C. Bottino
Summary: The study engineered metformin-loaded mesoporous silica nanospheres laden gelatin methacryloyl photocrosslinkable hydrogels, which demonstrated potential osteogenic effects on stem cells. Through testing mechanical properties, drug release, cytocompatibility, and osteogenic differentiation, the hydrogels showed promise for bone regeneration applications.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Yang-Hee Kim, Jonathan Dawson, Richard O. C. Oreffo, Yasuhiko Tabata, Dhiraj Kumar, Conrado Aparicio, Isha Mutreja
Summary: Musculoskeletal disorders pose a significant burden on the global economy and public health. Hydrogels, especially Gelatin methacryloyl (GelMA) hydrogel, hold great potential for enhancing the repair of damaged musculoskeletal tissues. This review provides a comprehensive overview of GelMA synthesis, hydrogel design, and its applications in regenerative medicine, particularly in the field of musculoskeletal drug delivery.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Taotao Liu, Yuzhuo Zhang, Mingyue Sun, Meiqi Jin, Wei Xia, Huazhe Yang, Tianlin Wang
Summary: This study investigated the effects of freezing conditions on GelMA hydrogels, finding that lower freezing temperatures and longer freezing times led to smaller pore sizes and enhanced cell viability and proliferation. The tunable microstructure of GelMA hydrogels can be achieved by regulating freezing conditions, offering promising prospects for tissue engineering applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mohammad B. Aljaber, Fiona Verisqa, Zalike Keskin-Erdogan, Kapil D. Patel, David Y. S. Chau, Jonathan C. Knowles
Summary: Approximately half of an adult human's body weight is made up of muscles. The source and bloom number of gelatin have been found to have an impact on the mechanical properties and biological activities of GelMA hydrogels.
Article
Polymer Science
Rebeca Leu Alexa, Horia Iovu, Jana Ghitman, Andrada Serafim, Cristina Stavarache, Maria-Minodora Marin, Raluca Ianchis
Summary: The focus of this study was on developing suitable GelMA hydrogel inks for 3D printing in tissue engineering applications. By exploring different degrees of GelMA methacrylation, concentrations of GelMA, and concentrations of photoinitiator, the researchers were able to determine the optimal formulations for the printing process. The presence of methacryloyl groups, the isoelectric point, hydrophilicity, mechanical properties, and structural integrity of the GelMA-based inks and 3D-printed scaffolds were thoroughly characterized to ensure their suitability for biological applications.
Article
Biochemistry & Molecular Biology
Margherita Burattini, Robrecht Lippens, Nicolas Baleine, Melanie Gerard, Joeri Van Meerssche, Chloe Geeroms, Jeremy Odent, Jean-Marie Raquez, Sandra Van Vlierberghe, Lieven Thorrez
Summary: In this study, Gelatin Methacryloyl (GelMA) is modified with hydrophilic 2-acryloxyethyltrimethylammonium chloride (AETA) and 3-sulfopropyl acrylate potassium (SPA) ionic comonomers to create a piezoionic hydrogel. The modified GelMA exhibits increased ionic conductivity and electrical response to mechanical stress, making it suitable for tissue engineering of skeletal muscles. The viability of murine myoblasts on the piezoionic hydrogels confirms their biocompatibility and the potential for utilizing piezo-effects in tissue engineering.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Dermatology
Hongyu Wang, Jiaming Wan, Zhiqiang Zhang, Ruixing Hou
Summary: Advancements in GelMA hydrogels are revolutionizing regenerative medicine and tissue engineering, with its potential applications in wound healing and skin regeneration. Challenges remain in scaling up 3D bioprinting techniques and developing advanced bioinks.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Engineering, Biomedical
Cigdem Bilici, Asena G. Tatar, Efsun Senturk, Caner Dikyol, Bahattin Koc
Summary: Recent studies have shown promising outcomes in 3D bioprinting of GelMA hydrogels with cell-laden structures for tissue engineering applications. However, the reliance on photo-induced gelation processes and the lack of alternative crosslinking methods have posed challenges. This study presents a novel approach using embedded bioprinting and bisulfite-initiated radical polymerization to form cell-laden GelMA hydrogel constructs, resulting in printed structures with high cell viability.
Article
Medicine, Research & Experimental
Ming Li, Dichao Huang, Hailin Yan, Haiyang Li, Liping Wang, Jianghui Dong
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2020)
Article
Engineering, Electrical & Electronic
Jianghui Dong, Ru-Jhang Jhu, Liping Wang, Cho-Pei Jiang, Cory J. Xian
MICROSYSTEM TECHNOLOGIES-MICRO-AND NANOSYSTEMS-INFORMATION STORAGE AND PROCESSING SYSTEMS
(2020)
Article
Medicine, Research & Experimental
Jiadong Pan, Miaozhong Li, Yaopeng Huang, Jianghui Dong, Xin Wang, Liping Wang
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2020)
Article
Medicine, Research & Experimental
Xiangyuan Wen, Hai Huang, Canbin Wang, Jianghui Dong, Xuezhi Lin, Fuming Huang, Hua Wang, Liping Wang, Shicai Fan
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
(2020)
Article
Orthopedics
Canbin Wang, Yuhui Chen, Liping Wang, Di Wang, Cheng Gu, Xuezhi Lin, Han Liu, Jiahui Chen, Xiangyuan Wen, Yuancheng Liu, Fuming Huang, Lufeng Yao, Shicai Fan, Wenhua Huang, Jianghui Dong
BMC MUSCULOSKELETAL DISORDERS
(2020)
Article
Cell Biology
Qunhu Zhang, Jie Li, You Li, Hui Che, Ying Chen, Jianghui Dong, Cory J. Xian, Dengshun Miao, Liping Wang, Yongxin Ren
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Oncology
Haiyan Cui, Yuanxiong Cheng, Yi He, Weiying Cheng, Wenqu Zhao, Haijin Zhao, Fiona H. Zhou, Liping Wang, Jianghui Dong, Shaoxi Cai
MOLECULAR MEDICINE REPORTS
(2020)
Article
Cell Biology
Haiqiang Lan, Tao Huang, Jiangwei Xiao, Zhaohong Liao, Jun Ouyang, Jianghui Dong, Cory J. Xian, Jijie Hu, Liping Wang, Yu Ke, Hua Liao
Summary: The study explores the antigenicity and immunogenicity of pH-magnetic dual-responsive nanocomposites in vivo. Results showed that CDMNP-PEG-CD induced a temporary blood monocyte response and was effectively cleared from the body through the urine system. Introduction of beta-CD and PEG/beta-CD polypseudorotaxane on SOMNP limited particle intramuscular dispersion and attenuated beta-CD-induced T cell response in implanted muscle.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Polymer Science
Wei-Chun Lin, Fang-Yu Fan, Hsing-Chung Cheng, Yi Lin, Yung-Kang Shen, Jing-Shiuan Lai, Liping Wang, Muhammad Ruslin
Summary: In this research, a shape-memory composite material was created using 3D printing technology. The study investigated the effects of various processing parameters on the deformation angle and recovery angle of the material. The results revealed that heating temperature was the most significant factor influencing both the deformation and recovery angle of the stimulus-responsive composite material.
Article
Endocrinology & Metabolism
Yu-Wen Su, Derick S. K. Wong, Jian Fan, Rosa Chung, Liping Wang, Yuhui Chen, Claire H. Xian, Lufeng Yao, Liang Wang, Bruce K. Foster, Jiake Xu, Cory J. Xian
Summary: This study identified the critical roles of BMP signaling in injury site bony repair and hypertrophic degeneration of the adjacent growth plate in a growth plate drill-hole repair model. Suppression of BMP signaling may attenuate undesirable bony repair at the injury site and suppress premature hypertrophy while potentially rescuing chondrogenesis at the adjacent growth plate.
Article
Chemistry, Multidisciplinary
Zipeng Yang, Chang-Peng Xu, Yuhui Chen, Wenqiang Li, Liping Wang, Zi-Guo Yuan
Summary: The study found that moderate-strength mechanical hydrogel stimulation may reduce AI-induced FLS inflammation via the NK-.B pathway. Different mechanical strength hydrogels were used to regulate synovium inflammation and reduce inflammatory factors and metalloproteinase expression in synovial tissues and intra-articular synovia.
Article
Pharmacology & Pharmacy
Cheng Yang, Yuhui Chen, Hai Huang, Shicai Fan, Chengliang Yang, Liping Wang, Wenqiang Li, Wenxin Niu, Jianwen Liao
Summary: The ROS-sensitive hydrogel prepared in this study showed strong free radical scavenging ability, accelerated wound healing, and promoted tissue regeneration in experiments.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Huaji Jiang, Jialiang Zhong, Wenjun Li, Jianghui Dong, Cory J. Xian, Yung-Kang Shen, Lufeng Yao, Qiang Wu, Liping Wang
Summary: The study found that GEN can promote osteogenesis of BMSCs by activating the BMP and Wnt/β-catenin pathways, providing a novel strategy for the treatment of osteoporosis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
