Article
Food Science & Technology
Han Du, Tao Ping, Wei Wu, Qingli Yang
Summary: This study prepared a simple and sensitive DNA tetrahedral fluorescence signal amplifier for sandwich detection of Staphylococcus aureus. The amplifier improved the fluorescence detection sensitivity and selectivity of S. aureus, and it may also be applicable to the detection of other food pathogens or environmental microorganisms in the future.
Article
Chemistry, Analytical
Lin Luo, Bao-Zhu Jia, Xiao-Qun Wei, Zhi-Li Xiao, Hong Wang, Yuan-Ming Sun, Yu-Dong Shen, Hong-Tao Lei, Zhen-Lin Xu
Summary: This study developed a fluorescence immunoassay for acrylamide detection, utilizing the fluorescent signal of carbon dots through a simple sample derivatization process with a detection limit lower than the legal limit set by WHO. The method demonstrated sensitivity and specificity to acrylamide, showing potential as an effective tool for monitoring acrylamide contamination in foods.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Spectroscopy
Qin Ouyang, Yongcun Yang, Shujat Ali, Li Wang, Huanhuan Li, Quansheng Chen
Summary: The study developed a rapid, sensitive, and specific aptasensor based on FRET for detecting Staphylococcus aureus in food. Under optimized conditions, there was a significant linear correlation between fluorescence intensity and S. aureus concentration, demonstrating high detection sensitivity and accuracy.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Microbiology
Pengfei Heng, Jiakai Liu, Zhen Song, Chuan Wu, Xiuzhong Yu, Yang He
Summary: In this study, a rapid and easy-to-operate detection method for Staphylococcus aureus was developed. The assay showed high specificity and sensitivity, able to detect S. aureus in only 25 minutes with a lower limit of detection compared to traditional PCR. The method also demonstrated high accuracy in detecting S. aureus in various bacterial samples, making it useful for timely diagnosis and prevention of S. aureus infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Optics
Maryam Poudineh, Sahar Poudineh, Tofigh Jalalifar, Sina Vakili, Amirabbas Rostami, Mohammad Samare-Najaf, Homayoon Fazeli Kakhaki, Amirreza Dehghanian, Mohamad Sabaghan, Hamid Behrouj
Summary: The present study aimed to design a novel detection technique for Staphylococcus aureus Alpha-toxin using aptamer-localized surface plasmon resonance (LSPR). The results demonstrated the successful synthesis and distribution of gold nanoparticles (GNPs), and the optimized performance of the probe and aptamer in detecting different concentrations of alpha-toxin. The aptasensor showed a desired performance in terms of linear range, limit of detection, limit of quantification, percent recovery, and relative standard deviation.
Article
Food Science & Technology
Yue-Hong Pang, Yu-Xin Wang, Wan-Ming Chen, Xiao-Fang Shen, Yi Zhang
Summary: A method for rapid detection of Staphylococcus aureus using CSDPR was proposed in this study, with a detection limit of 9.5 x 10(4) CFU/mL and a reaction time of 15 minutes. The method was successfully applied to spiked milk and steak samples with consistent results compared to plate counting method.
Article
Chemistry, Multidisciplinary
Xiaoqi Tao, Ziyi Liao, Yaqing Zhang, Fei Fu, Mengqi Hao, Yang Song, Erqun Song
Summary: A one-step FRET sensor was developed for specific and sensitive detection of Staphylococcus aureus in food and serum samples. The sensor exhibited a good detection range and low detection limit, as well as good recovery rates and low relative standard derivations in real samples.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Kien Hong Trinh, Ulhas Sopanrao Kadam, Jinnan Song, Yuhan Cho, Chang Ho Kang, Kyun Oh Lee, Chae Oh Lim, Woo Sik Chung, Jong Chan Hong
Summary: The study successfully isolated DNA aptamers that can bind to fenitrothion through the SELEX process. The FenA2 aptamer exhibited high detection sensitivity and specificity, providing a potential label-free detection mode for real-time detection of fenitrothion. This opens up new possibilities for analytical tools in various samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Pernille Ommen, Line Hansen, Bente K. Hansen, Hieu Vu-Quang, Jorgen Kjems, Rikke L. Meyer
Summary: In this study, an aptamer-targeted liposomal drug delivery system was developed to eradicate Staphylococcus aureus biofilm infections in vitro.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Azam Bagheri Pebdeni, Maryam Mousavizadegan, Morteza Hosseini
Summary: The proposed dual detection sensor combines aptamer and antibiotic for effective recognition and quantification of Staphylococcus aureus. The sensor showed high specificity in detecting S. aureus using CuNCs, with potential applications for monitoring pathogenic bacteria.
Article
Chemistry, Analytical
Afang Zhu, Shujat Ali, Zhen Wang, Tianhui Jiao, Qin Ouyang, Quansheng Chen
Summary: A highly structured SERS biosensor was developed for the detection of Staphylococcus aureus. The biosensor utilized a chemically modified PDMS film as the SERS substrate, with self-assembled Au@Ag NPs for enhancing detection sensitivity. The bacterial surface was covered with Au@Ag NPs, resulting in more hot spots for improved detection. The biosensor achieved an ultra-low detection response of 16 cfu/mL and showed satisfactory results in the detection of S. aureus in tap water, milk, and fish samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Huanli Gao, Tingting Xu, Jiahuan Zhou, Orlando J. Rojas, Ming He, Xingxiang Ji, Hongqi Dai
Summary: This article introduces a system for the rapid and ultra-sensitive detection of the prevalent foodborne pathogen Staphylococcus aureus. By utilizing a dual-aptamer-based sandwich immunobiosensor, the system demonstrates specificity and anti-fouling capacity, with signal amplification through highly conductive gold nanoparticles. Experimental results show that the immunobiosensor has an extended detection range and low detection limit, while maintaining stability for two weeks.
Article
Biophysics
Yi Xu, Peihuan He, Waqas Ahmad, Md Mehedi Hassan, Shujat Ali, Huanhuan Li, Quansheng Chen
Summary: This study presents a novel strategy for the detection of Staphylococcus aureus using a hybridization chain reaction-assisted surface enhanced Raman scattering approach. The developed method demonstrated high sensitivity and rapid detection capability, making it suitable for food safety and biomedical research applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Qin Ouyang, Baoning Wang, Waqas Ahmad, Yongcun Yang, Quansheng Chen
Summary: In this study, a novel upconversion fluorescent biosensor was designed to detect Staphylococcus aureus using fluorescence resonance energy transfer. The biosensor showed a low limit of detection and a linear calibration plot under optimal conditions. The results from detecting S. aureus in real samples showed no significant difference compared to the conventional plate count approach.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Min Chen, Lu Han, Dandan Zhou, Liangyu Kong, Leiqing Pan, Kang Tu
Summary: A fluorescence sensor composed of ADA@UCNPs and MTX combined with PCR was developed for rapid, specific and ultrasensitive determination of Salmonella typhimurium. The sensor improved sensitivity for detection of Salmonella typhimurium through inner filter effect and PCR amplification process, achieving a detection limit of 11 CFU/mL. Additionally, the sensor was successfully applied to chicken samples with satisfactory results.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)