期刊
MICROCHIMICA ACTA
卷 185, 期 11, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-3022-0
关键词
Mycotoxin; Rapid detection; Upconversion nanoparticles; Wheat, beer
资金
- Key Research and Development Program of Jiangsu Province [BE2016306]
- National Natural Science Fund of China [NSFC 31772086]
- China Postdoctoral Science Foundation [2016T90430]
- Jiangsu Province Postdoctoral Science Foundation [1601087B, 1701097B]
- Young Elite Scientists Sponsorship Program by CAST [2017QNRC001]
- Fundamental Research Funds for the Central Universities [JUSRP21826]
An aptamer-based test strip is described for visual and instrumental determination of the mycotoxin ochratoxin A (OTA). It is based on the use of NaYF4:Yb,Er upconversion nanoparticles (UCNPs) as a label for the aptamer and on the competition between OTA and its complementary sequence for an OTA-specific aptamer. To improve the analytical performance, the optical properties of the UCNPs, the fluidity of the UCNP-aptamer conjugate, and the migration rate on the nitrocellulose membranes were investigated. Under the optimal experimental conditions and by using a 980-nm laser, the relative fluorescence intensity (test line value/control line value) is proportional to the logarithm of the OTA concentration over a range from 5 to 100 ng(.)mL(-1) (R-2 = 0.9955). The limit of the detection is 1.86 ng(.)mL(-1). This aptamer based flow assay can be performed within 15 min and has no serious cross-sensitivity to potentially interfering species. It was successfully applied to the determination of OTA in spiked wheat and beer samples.
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