期刊
MICROCHIMICA ACTA
卷 185, 期 11, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-018-3028-7
关键词
Tungsten diselenide nanosheets; Electrochemical biosensor; Sandwiched structure; Protein; Signal enhancement
资金
- National Natural Science Foundation of China [21475115]
- Henan Provincial Science and technology innovation team [C20150026]
- Nanhu Scholars Program of XYNU
- Henan Science and Technology Cooperation Project [172106000064]
A sensitive aptamer/protein binding-triggered sandwich assay for thrombin is described. It is based on electrochemical-chemical-chemical redox cycling using a glassy carbon electrode (GCE) that was modified with WSe2 and gold nanoparticles (AuNPs). The AuNPs are linked to thrombin aptamer 1 via Au-S bonds. Thrombin is first captured by aptamer 1 and then sandwiched through the simultaneous interaction with AuNPs modified with thrombin-specific aptamer 2 and signalling probe. Subsequently, the DNA-linked AuNP hybrids result in the capture of streptavidin-conjugated alkaline phosphatase onto the modified GCE through the specific affinity reaction for further signal enhancement. As a result, a linear range of 0-1 ng mL(-1) and a detection limit as low as 190 fg mL(-1) are accomplished. The specificity for thrombin is excellent. Conceivably, this strategy can be easily expanded to other proteins by using the appropriate aptamer.
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