期刊
METHODS
卷 156, 期 -, 页码 66-78出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2018.12.007
关键词
A-to-I RNA editing; ICE-seq; High-throughput sequencing
资金
- Ministry of Education, Science, Sports, and Culture of Japan (MEXT)
- Japan Society for the Promotion of Science (JSPS)
In A-to-I RNA editing, adenosine is converted to inosine in double-stranded regions of RNAs. Inosine, an abundant epitranscriptomic mark, contributes to a wide range of biological processes by regulating gene expression post-transcriptionally. To understand the effect of A-to-I RNA editing on regulation of the epitran-scriptome, accurate mapping of inosines is necessary. To this end, we established a biochemical method called inosine chemical erasing sequencing (ICE-seq) that enables unbiased and reliable identification of A-to-I RNA editing sites throughout the transcriptome. Here, we describe our updated protocol for ICE-seq in the human transcriptome.
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