Article
Materials Science, Biomaterials
Caroline S. Taylor, Mehri Behbehani, Adam Glen, Pooja Basnett, David A. Gregory, Barbara B. Lukasiewicz, Rinat Nigmatullin, Frederik Claeyssens, Ipsita Roy, John W. Haycock
Summary: The use of nerve guidance conduits (NGCs) is an effective method for treating peripheral nerve injuries. However, NGCs lack the specific guidance cues found in nerve grafts and are not suitable for treating large gap injuries. The use of intraluminal aligned fiber guidance scaffolds has been shown to enhance neuronal cell neurite outgrowth and Schwann cell migration distances.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Caroline S. Taylor, Joseph Barnes, Manohar Prasad Koduri, Shamsal Haq, David A. Gregory, Ipsita Roy, Raechelle A. D'Sa, Judith Curran, John W. Haycock
Summary: Silane modification is a cost-effective method to modify existing biomaterials for tissue engineering. This study reports the deposition of CL11 onto PCL scaffolds for peripheral nerve regeneration. The modified fibers significantly support cell viability and differentiation, indicating their potential for improving nerve regeneration.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Lorena R. Lizarraga-Valderrama, Giulia Ronchi, Rinat Nigmatullin, Federica Fregnan, Pooja Basnett, Alexandra Paxinou, Stefano Geuna, Ipsita Roy
Summary: The study demonstrated that PHA-NGCs are highly promising in promoting peripheral nerve regeneration, showing comparable outcomes to autografts in functional evaluation and nerve morphology.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hongyun Xuan, Biyun Li, Feng Xiong, Shuyuan Wu, Zhuojun Zhang, Yumin Yang, Huihua Yuan
Summary: In this study, well-aligned poly (L-lactic acid) nanofibers with varied nano-porous surface structures were designed, showing that larger nano-pores were less affected by Staphylococcus aureus and more favorable for the proliferation and differentiation of neural stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Liangfu Jiang, Xingyu Ouyang, Dupiao Zhang, Gang Wang, Zhe Zhang, Wei Wang, Hede Yan
Summary: This study presents a Gel-Ppy modified nerve conduit with excellent biocompatibility and conductivity, and evaluates its performance in promoting nerve regeneration in vitro and in vivo. The results demonstrate that Gel-Ppy modified nerve conduit can significantly enhance Schwann cell proliferation, contribute to Schwann cell regeneration in vivo, and improve axon diameters and myelin sheath thickness, leading to the amelioration of muscle atrophy, nerve conduction, and motor function recovery. Further investigation indicates that the Gel-Ppy conduit facilitates nerve regeneration by upregulating the Rap1 pathway to induce neurite outgrowth. Therefore, Gel-Ppy modified nerve conduit can provide a favorable microenvironment for nerve regeneration and be popularized as a novel therapeutic strategy for peripheral nerve injury.
Article
Engineering, Biomedical
Xianhao Dong, Siyang Liu, Yueyue Yang, Shan Gao, Wenlei Li, Jiasong Cao, Ye Wan, Ziqi Huang, Guanwei Fan, Quan Chen, Hongjun Wang, Meifeng Zhu, Deling Kong
Summary: Research on nerve conduits composed of oriented microfiber-bundle cores and randomly organized nanofiber sheaths has shed light on the regulatory role of microfiber orientation in promoting peripheral nerve regeneration, elucidating a cascade of biological responses and ultimately enhancing nerve regeneration outcomes.
Article
Chemistry, Multidisciplinary
Yangnan Hu, Zhuoyue Chen, Hongyang Wang, Jiahui Guo, Jiaying Cai, Xiaoyan Chen, Hao Wei, Jieyu Qi, Qiuju Wang, Huisheng Liu, Yuanjin Zhao, Renjie Chai
Summary: Inspired by the structures of peripheral nerves, researchers have developed a conductive topological scaffold for nerve repair using modified butterfly wings. The scaffold showed increased neurite length and guided cellular orientation, and successfully repaired nerve defects in rats.
Review
Chemistry, Multidisciplinary
Faranak Mankavi, Rana Ibrahim, Hongjun Wang
Summary: This article summarizes the recent advances in nerve guidance conduits (NGCs) for peripheral nerve regeneration and repair, including biomaterial innovations, structural design, advanced fabrication technologies, and cellular responses to biomimetic cues.
Article
Engineering, Biomedical
Papon Muangsanit, Victoria Roberton, Eleni Costa, James B. Phillips
Summary: Vascularisation is crucial for nerve tissue engineering to support long-term survival of implanted cells. Constructs containing endothelial cells have shown to promote axonal regeneration and vascularisation across peripheral nerve gaps.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Environmental
Hamed Alipour, Ahmad Saudi, Hosein Mirazi, Mohammad Hossein Kazemi, Omid Alavi, Zahra Zeraatpisheh, Sareh Abolhassani, Mohammad Rafienia
Summary: Vitamin C is an essential supplement that has been widely used for therapeutic purposes. This study developed electrospun fibers containing different concentrations of vitamin C for peripheral nerve tissue engineering. The results showed that increasing vitamin C content enhanced the mechanical properties and cell viability of the fibers. These findings suggest the potential applicability of vitamin C-containing scaffolds in nerve tissue engineering.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Nanoscience & Nanotechnology
Feng Xiong, Shuo Wei, Shuyuan Wu, Wei Jiang, Biyun Li, Hongyun Xuan, Ye Xue, Huihua Yuan
Summary: In this study, a biomimetic piezoelectric and conductive nanofiber scaffold was constructed for the repair of large peripheral nerve deficits, which promoted nerve cell differentiation and nerve fiber extension. The application of the scaffold in rat sciatic nerve injury prevented autotomy and facilitated nerve regeneration and functional recovery.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Pharmacology & Pharmacy
Yasaman Mozhdehbakhsh Mofrad, Amir Shamloo
Summary: Injectable conductive hydrogels are a promising treatment option for nervous system injuries, but research in this area is limited. This study developed a chitosan/beta-glycerophosphate/salt hydrogel with added conductive aligned nanofibers to improve its biochemical and biophysical properties, inspired by natural nerve tissue. The results showed that the degradation rate of the hydrogels is proportional to axon regrowth. Additionally, the mechanical and electrical properties of these hydrogels, as well as the interconnective structure of the scaffolds, were found to support cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Engineering, Biomedical
Qingqing Lu, Feng Zhang, Weinan Cheng, Xiang Gao, Zhaozhao Ding, Xiaoyi Zhang, Qiang Lu, David L. Kaplan
Summary: The study utilized modular assembly for fabricating nerve conduits with multifunctional guidance cues, which promoted cell proliferation and growth factor secretion, and demonstrated improved nerve regeneration in both cell and animal experiments for rat sciatic nerve defects.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Polymer Science
Anamika Singh, Parvaiz Ahmad Shiekh, Irfan Qayoom, Ekta Srivastava, Ashok Kumar
Summary: This study developed a therapeutic approach for treating traumatic peripheral nerve injuries after diabetic neuropathy by utilizing nerve guiding scaffold and stem cell-derived exosomes. Both in vitro and in vivo experiments demonstrated significant improvement in nerve injuries after diabetic peripheral neuropathy, suggesting the potential clinical application of this approach.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Engineering, Biomedical
Yaowei Xuan, Lin Li, Xuelai Yin, Dongming He, Siyao Li, Chenping Zhang, Yuan Yin, Wanlin Xu, Zhen Zhang
Summary: This study developed a nerve guidance conduit (NGC) based on bredigite (BRT) bioceramic, which could promote the repair of peripheral nerve injury. BRT bioceramic showed good biocompatibility and sustained release of beneficial ions for nerve repair. The experiments demonstrated that BRT-incorporating NGC could promote cell proliferation, myelination, and vascular formation, as well as improve electrophysiological performance and muscle atrophy in a rat sciatic nerve defect model.
ADVANCED HEALTHCARE MATERIALS
(2023)