Article
Immunology
Thyago Bispo Leonel, Joel Jose Megale Gabrili, Carla Cristina Squaiella-Baptistao, Trent M. Woodruff, John D. Lambris, Denise V. Tambourgi
Summary: This study analyzed the effects of Bothrops jararaca snake venom on the complement system and innate immunity. The venom was found to activate the complement system and induce inflammatory responses. Inhibition of the complement system may represent a potential therapeutic approach for Bothrops jararaca envenoming.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mauricio Frota Camacho, Daniel R. Stuginski, Debora Andrade-Silva, Milton Y. Nishiyama-Jr, Richard H. Valente, Andre Zelanis
Summary: Snake venom protein synthesis in the venom gland undergoes highly regulated processes, which occur within a defined period and specific cellular locations. Subcellular proteome analysis of B. jararaca venom gland identified a conserved core proteome among different life stages and between sexes. The highly abundant proteins in the venom gland showed similarities with highly expressed genes in human salivary glands, suggesting a conserved core signature of salivary gland secretory epithelium. The newborn venom gland displayed a unique expression signature, potentially reflecting the ontogenetic development constraints and contributing to venom proteome diversity.
Article
Food Science & Technology
Carolina Yukiko Kisaki, Stephanie Santos Suehiro Arcos, Fabio Montoni, Wellington da Silva Santos, Hamida Macedo Calacina, Ismael Feitosa Lima, Daniela Cajado-Carvalho, Emer Suavinho Ferro, Milton Yutaka Nishiyama-Jr, Leo Kei Iwai
Summary: This study investigated quantitative proteomic changes in breast tumor cell lines following treatment with Bothrops jararaca snake venom, showing differential expression of proteins related to cancer cell metabolism, immune response, and inflammation. The data suggest that sub-toxic doses of B. jararaca venom have the potential to modulate cancer-related protein targets in cancer cells, illustrating a novel biochemical strategy to identify therapeutic targets for cancer cell growth and survival.
Article
Pharmacology & Pharmacy
Roney Vander dos Santos, Giovanna Grillo, Henrique Fonseca, Danijela Stanisic, Ljubica Tasic
Summary: 90% of snakebite envenomation in Brazil is caused by Bothrops snakes from the Viperidae family, with a serine protease from Bothrops jararaca chosen for study. The study found that hesperetin can act as a mixed inhibitor for the serine protease from Bothrops jararaca snake venom, potentially helping to reverse on-site blood clotting problems after snakebite accidents. Further research is needed to determine the full impact of hesperetin in this context.
Article
Food Science & Technology
Lilian Rumi Tsuruta, Ana Maria Moro, Denise V. Tambourgi, Osvaldo Augusto Sant'Anna
Summary: Oral tolerance is a process of specific suppression of immune responses through prior oral administration of an antigen, with unique immunological importance. This study found that inducing oral tolerance to B. jararaca venom in mice led to differences in antibody levels, as well as variations in cross-reactivity with toxins of other Bothrops species.
Article
Biochemistry & Molecular Biology
Rafael J. Borges, Guilherme H. M. Salvador, Henrique B. Campanelli, Daniel C. Pimenta, Mario de Oliveira Neto, Isabel Uson, Marcos R. M. Fontes
Summary: Phospholipases A(2) are commonly found in venomous snake families and can cause serious consequences in snakebites, requiring prompt antivenom administration. Bothrops jararacussu venom contains BthTX-II as a major component, with two variants a and b showing structural and functional differences. The variants exist as monomers in neutral conditions and dimers in acidic environments, maintaining catalytic activity in both states.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Pharmacology & Pharmacy
Ganesh H. Sampat, Kashinath Hiremath, Jagadeesh Dodakallanavar, Vishal S. Patil, Darasaguppe R. Harish, Prakash Biradar, Rashmi K. Mahadevamurthy, Manish Barvaliya, Subarna Roy
Summary: Snake bite is a neglected disease that affects millions of people worldwide. Snake venom, composed of proteins and enzymes, has diverse pharmacological actions. Traditional medicine, using plant-derived chemicals, can neutralize the adverse effects of snake venom. Small molecules and secondary metabolites have been found effective in inhibiting snake venom enzymes.
PHARMACOLOGICAL REPORTS
(2023)
Article
Infectious Diseases
Camila Martos Thomazini, Ana Teresa Azevedo Sachetto, Cynthia Zaccanini de Albuquerque, Vania Gomes de Moura Mattaraia, Ana Karina de Oliveira, Solange Maria de Toledo Serrano, Ivo Lebrun, Katia Cristina Barbaro, Marcelo Larami Santoro
Summary: Envenomation by snakebites, particularly from the lance-headed snake Bothrops jararaca in southeastern Brazil, causes a fall in blood platelet counts. The venom disrupts blood platelets in a complex manner, with botrocetin and other venom compounds playing a role in this process. Von Willebrand factor is affected by the venom, but it is not the main mechanism leading to decreased platelet counts.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Toxicology
Felipe Silva de Franca, Joel Jose Megale Gabrili, Laurence Mathieu, Francois Burgher, Joel Blomet, Denise V. Tambourgi
Summary: The study investigated the systemic events in human whole-blood model induced by Bothrops lanceolatus venom, revealing a strong inflammatory reaction characterized by complement activation and release of lipid mediators that may favor thrombi formation.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Immunology
Fernanda V. Kondo, Wafa H. K. Cabrera, Orlando G. Ribeiro, Marcelo De Franco, Jose Ricardo Jensen, Gisele Picolo, Morena B. Sant'Anna, Monica Spadafora-Ferreira, Andrea Borrego, Olga M. Ibanez, Nancy Starobinas
Summary: In this study, the role of mast cells and the interference of host genetic background in the inflammation induced by Bothrops jararaca venom (BjV) were investigated using mouse strains selected for maximal or minimal acute inflammatory response (AIR). The findings suggest that mast cells play an important role in pain, neutrophil migration, and reactive oxygen species (ROS) production triggered by BjV, particularly in mouse strains that are more susceptible to the action of BjV.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Carlos Alberto-Silva, Samyr Machado Querobino, Cesar Augusto Melo-Silva, Maricilia Silva Costa, Luis Vicente Franco Oliveira, Stella Regina Zamuner
Summary: This study investigated the impact of low molecular weight peptides in the venom of Bothrops jararaca snake on lung mechanics and inflammation in mice. The results showed that both Bj-CV and Bj-PF injections led to changes in lung function and increased muscle and pulmonary inflammation.
Article
Biochemistry & Molecular Biology
Victor David, Luciana Serrao Wermelinger, Flavia Serra Frattani, Antonio Gilcler Ferreira Lima, Yasmyn Fernandes Silva Santos, Paulo Antonio de Souza Mourao, Fabio Ceneviva Lacerda Almeida, Eleonora Kurtenbach, Russolina Benedeta Zingali
Summary: This study expresses and analyzes the rJararacin protein, which has been found to effectively inhibit the adhesion of B16F10 cells and platelets to the fibronectin matrix, and inhibit platelet aggregation induced by ADP, collagen, and thrombin. Furthermore, rJararacin can effectively prevent platelet aggregation and thrombus formation in vitro and in vivo. These results suggest that rJararacin has the potential as an αIIbβ3 antagonist to prevent arterial thrombosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemical Research Methods
Joeliton S. Cavalcante, Weslley Ruan G. Borges da Silva, Laudiceia Alves de Oliveira, Ingrid Mayara C. Brito, Kevin S. Muller, Ivynna Suellen J. Vidal, Lucilene Delazari dos Santos, Roberta Jeane Bezerra Jorge, Cayo Almeida, Carla de Lima Bicho
Summary: This study investigated the edema and plasma protein expression changes induced by B. erythromelas venom in mice, revealing potential biomarkers and therapeutic targets for monitoring and treating snakebites.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Joeliton dos Santos Cavalcante, Cayo Antonio Soares de Almeida, Milan Avila Clasen, Emerson Lucena da Silva, Luciana Curtolo de Barros, Aline Diogo Marinho, Bruno Cesar Rossini, Celso Luis Marino, Paulo Costa Carvalho, Roberta Jeane Bezerra Jorge, Lucilene Delazari dos Santos
Summary: This study explores the pathophysiological effects of Bothrops leucurus snakebite and the organism's reaction. By analyzing plasma proteins, it was found that B. leucurus venom triggers a thromboinflammation scenario and is related to inflammation, complement and coagulation systems, platelets and neutrophils, lipid and retinoid metabolism, oxidative stress and tissue repair.
JOURNAL OF PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Diego Dantas Almeida, Vincent Louis Viala, Pedro Gabriel Nachtigall, Michael Broe, H. Lisle Gibbs, Solange Maria de Toledo Serrano, Ana Maria Moura-da-Silva, Paulo Lee Ho, Milton Yutaka Nishiyama-Jr, Inacio L. M. Junqueira-de-Azevedo
Summary: Venom is a key adaptive innovation in snakes, and the recruitment of nonvenom genes to become part of the toxin arsenal is a significant evolutionary question. Through genomic analysis of the pitviper Bothrops jararaca, it was found that certain toxin families expanded near their nonvenomous ancestors, suggesting a locally assembled venom arsenal where clinically relevant toxins expanded through gene duplications or co-options.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Norival Alves Santos-Filho, Gabriela Marinho Righetto, Marina Rodrigues Pereira, Julia Pinto Piccoli, Larissa Mathias Teizen Almeida, Thaina Cristina Leal, Ilana Lopes Baratella Cunha Camargo, Eduardo Maffud Cilli
Summary: A stable and shorter peptide, synthesized by replacing a cysteine residue with a lysine, showed superior antimicrobial activity compared to the original peptide, suggesting that the amino acid substitutions enhanced the peptide's antibacterial properties.
Article
Biochemistry & Molecular Biology
Natasha Marques Cassani, Igor Andrade Santos, Victoria Riquena Grosche, Giulia Magalhaes Ferreira, Marco Guevara-Vega, Rafael Borges Rosa, Lindomar Jose Pena, Nilson Nicolau-Junior, Adelia Cristina Oliveira Cintra, Tiago Patriarca Mineo, Robinson Sabino -Silva, Suely Vilela Sampaio, Ana Carolina Gomes Jardim
Summary: This study demonstrates that two phospholipases A2 (PLA2) isolated from snake venom can inhibit the activity of Zika virus, with a strong effect on the entry of the virus into host cells. These findings suggest that these PLA2s could serve as potential templates for the development of antiviral drugs against Zika virus in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Infectious Diseases
Thais P. Nascimento, Alexandre Vilhena Silva-Neto, Djane Clarys Baia-da-Silva, Patricia Carvalho da Silva Balieiro, Antonio Alcirley da Silva Baleiro, Jacqueline Sachett, Lisele Brasileiro, Marco A. Sartim, Flor Ernestina Martinez-Espinosa, Fan Hui Wen, Manuela B. Pucca, Charles J. Gerardo, Vanderson S. Sampaio, Priscila Ferreira de Aquino, Wuelton M. Monteiro
Summary: Snakebite envenomations during pregnancy in the Brazilian Amazon increase the risk of fetal and neonatal deaths, despite the low incidence of snakebites in pregnant women.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Pharmacology & Pharmacy
Natalia Vitoria Bitencourt, Gabriela Marinho Righetto, Ilana Lopes Baratella Cunha Camargo, Mariana Ortiz de Godoy, Rafael Victorio Carvalho Guido, Glaucius Oliva, Norival Alves Santos-Filho, Eduardo Maffud Cilli
Summary: Recent studies have found that the peptide [des-Cys(11),Lys(12),Lys(13)-(p-BthTX-I)(2)K] (p-Bth) is a potent analog with enhanced antimicrobial activity and inhibitory effects against SARS-CoV-2. The dimerization and dendrimerization of p-Bth are crucial for its antibacterial activity, while the inhibitory activity against PLpro remains consistent regardless of the structure. These findings highlight the importance of dimerization and dendrimerization in the development of antimicrobial peptides.
Article
Biochemistry & Molecular Biology
Fernanda Gobbi Amorim, Thiago Abrahao Silva, Gabriela de Oliveira Almeida, Damien Redureau, Hamilton Cabral, Loic Quinton, Suely Vilela Sampaio
Summary: We have successfully isolated the first phosphodiesterase from Bothrops jar-aracussu, which we named Bj-PDE. This enzyme showed stable enzymatic activity at different storage temperatures and was positively influenced by Ca2+ and Mg2+ ions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Marco A. Sartim, Renato C. Nogueira, Tavila T. A. Cavalcante, Lucas O. Sousa, Wuelton M. Monteiro, Adelia C. O. Cintra, Evandro M. Neto-Neves, Suely V. Sampaio
Summary: This study aimed to evaluate the hemodynamic effects of Crotalus durissus snake venom (CTX) using in vivo and ex vivo approaches in a rat model. The results showed that CTX reduced blood pressure, heart rate, and left ventricle function in vivo, but had no effect on ex vivo heart function. In vitro experiments demonstrated that CTX binds to myoblast cell line, and this binding can be mitigated by a specific antagonist. These findings are important for the clinical management of cardiovascular complications caused by Crotalus durissus snakebite.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Jonatan C. S. de Carvalho, Pedro V. da Silva-Neto, Diana M. Toro, Carlos A. Fuzo, Viviani Nardini, Vinicius E. Pimentel, Malena M. Perez, Thais F. C. Fraga-Silva, Camilla N. S. Oliveira, Augusto M. Degiovani, Fatima M. Ostini, Marley R. Feitosa, Rogerio S. Parra, Jose J. R. da Rocha, Omar Feres, Fernando C. Vilar, Gilberto G. Gaspar, Isabel K. F. M. Santos, Ana P. M. Fernandes, Sandra R. Maruyama, Elisa M. S. Russo, Vania L. D. Bonato, Cristina R. B. Cardoso, Marcelo Dias-Baruffi, Lucia H. Faccioli, Carlos A. Sorgi
Summary: COVID-19 is associated with immune dysregulation, and glucocorticoids (GCs) have attracted attention for their role in inhibiting pro-inflammatory cytokines. The endocannabinoid (eCB) system also regulates immune response. However, the effect of GCs on the lipid pathway in COVID-19 therapy is still unknown.
Review
Food Science & Technology
Zeca M. Salimo, Andre L. Barros, Asenate A. X. Adriao, Aline M. Rodrigues, Marco A. Sartim, Isadora S. de Oliveira, Manuela B. Pucca, Djane C. Baia-da-Silva, Wuelton M. Monteiro, Gisely C. de Melo, Hector H. F. Koolen
Summary: Malaria is a global public health problem caused by Plasmodium spp. and transmitted by female mosquitoes. Drug therapies and vector control are commonly used methods, but drug resistance is a challenge. Animal venoms have potential as a source for new antimalarial molecules. This review summarizes antimalarial toxins from animal venoms.
Review
Biochemistry & Molecular Biology
Joeliton S. Cavalcante, Denis Emanuel Garcia de Almeida, Norival A. Santos-Filho, Marco Aurelio Sartim, Amanda de Almeida Baldo, Lisele Brasileiro, Polianna L. Albuquerque, Samella S. Oliveira, Jacqueline Almeida Goncalves Sachett, Wuelton Marcelo Monteiro, Rui Seabra Ferreira Jr
Summary: Snakebite envenoming is classified as a neglected disease due to its significant health impact in tropical and subtropical countries. Bothrops genus snakes in Latin America are the main culprits for snakebites, causing local and systemic inflammation, thrombotic and hemorrhagic events, and various complications. The interface between inflammation and coagulation in snakebite envenoming is still not well-studied.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Tavila Tatiane Amorim Cavalcante, Marcia Beatriz Silva de Souza, Juliana Costa Ferreira Neves, Hiochelson Najibe Santos Ibiapina, Fabiane Bianca Albuquerque Barbosa, Karolaine Oliveira Bentes, Eliane Campos Alves, Hedylamar Oliveira Marques, Monica Colombini, Suely Vilela Sampaio, Manuela Berto Pucca, Iran Mendonca da Silva, Luiz Carlos de Lima Ferreira, Vanderson de Souza Sampaio, Ana Maria Moura-da-Silva, Allyson Guimaraes Costa, Wuelton Marcelo Monteiro, Jacqueline Almeida Goncalves Sachett, Marco Aurelio Sartim
Summary: Bothrops snakebite envenomation is a significant health issue in the Brazilian Amazon. Secondary infections are common complications, which affect local manifestations and inflammatory response.
Article
Cell Biology
Diana Mota V. Toro, Pedro V. da Silva-Neto, Jonatan C. S. A. de Carvalho, Carlos A. M. Fuzo, Malena M. Perez, Vinicius E. Pimentel, Thais F. C. Fraga-Silva, Camilla N. S. R. Oliveira, Glaucia R. Caruso, Adriana F. L. Vilela, Pedro V. Nobre-Azevedo, Thiago V. Defelippo-Felippe, Jamille G. M. M. Argolo, Augusto M. Degiovani, Fatima M. R. Ostini, Marley R. S. Feitosa, Rogerio S. C. Parra, Fernando C. G. Vilar, Gilberto G. Gaspar, Jose J. R. da Rocha, Omar P. Feres, Gabriel P. Costa, Sandra R. C. Maruyama, Elisa M. S. Russo, Ana Paula M. Fernandes, Isabel K. F. M. Santos, Adriana T. Malheiro, Ruxana T. Sadikot, Vania L. D. Bonato, Cristina R. B. Cardoso, Marcelo Dias-Baruffi, Atila A. Trape, Lucia H. A. Faccioli, Carlos A. Sorgi, ImmunoCovid Consortium Grp
Summary: SARS-CoV-2 infection leads to distinct disease patterns with significant changes in host regulatory responses. Severe cases suffer from lung inflammation and systemic effects, while critically ill patients experience a lipid storm that affects inflammation and tissue damage. Sphingolipids play critical roles in various cellular processes and may serve as potential biomarkers and therapeutic targets for COVID-19.
Article
Biochemistry & Molecular Biology
Carolina P. Bernardes, Neife A. G. Santos, Tassia R. Costa, Danilo L. Menaldo, Flavia M. Sisti, Martin K. Amstalden, Diego L. Ribeiro, Lusania M. G. Antunes, Suely V. Sampaio, Antonio C. Santos
Summary: In this study, the snake-venom-based tripeptide BTX-I was chemically modified to enhance its neurotrophic and neuroprotective effects. The ethyl-esterified peptide BTX-II showed significant improvement in promoting neuritogenesis and upregulating proteins and genes associated with cognitive improvements.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Adelia Cristina Oliveira Cintra, Tassia Rafaella Costa, Gabriel Neves Cezarette, Fabiola Attie de Castro, Daniel Carvalho Pimenta, Suely Vilela Sampaio
Summary: Venom peptides from Bothrops atrox snake venom, particularly the novel peptide Batroxin I, showed selective cytotoxicity towards HepG2 liver tumor cells and induced apoptosis by intrinsic pathway activation. The amino acid sequence of Batroxin I was determined and it belonged to the class of bradykinin-enhancing peptides and cell penetration peptide. This peptide could potentially be used as a template for drug development and as an adjuvant to treat cancer and other diseases.
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
(2023)