期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 43, 页码 29261-29269出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b08542
关键词
RNA interference therapy; small interfering RNA; self-assembly; polyethylenimine; nanoparticle
资金
- National Basic Research Program of China (973 Program) [2015CB755500]
- National Natural Science Foundation of China (NSFC) [51203177, 81471778, 61302040, 81602499]
- National High Technology Research and Development Program of China (863 Program) [2014AA020708]
- Science and Technology Foundation of Shenzhen, China [SFG2012.566, SKC2012.237, JCYJ20140417113430662]
RNA interference (RNAi) therapy is a promising treatment for various diseases. However, its application is still restricted by the lack of efficient and safe delivery systems. A novel siRNA delivery vehicle based on epsilon-caprolactone-modified polyethylenimine (PEI-CL) is presented here. The PEI-CL macromolecules with different grafting degrees were synthesized via a simple ring-opening reaction. This macromolecule strongly protects the siRNA from degradation in serum and promotes the cellular uptake and endosomal escape detected via chemical exchange saturation transfer magnetic resonance analysis and fluorescence imaging. The in vivo measurement was performed with HCT-116 colon tumor xenograft that stably expressed luciferase. The data showed that the PEI-CL/siRNA nanocomplexes elicited strong RNAi response. More interestingly, enhanced gene transfection efficiency was achieved by simultaneous cotransfection with siRNA and DNA plasmid via this novel nanosystem. Overall, our study suggests the PEI-CL macromolecule with great promise for siRNA delivery.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据