Article
Chemistry, Analytical
Limei Zhang, Zhang Zhang, Jingling Xie, Yuanqing Zhao, Gang Tian, Hui Jiang, Hualin Tao, Jinbo Liu
Summary: A novel fluorescent sensor combining toehold-mediated target invasion and DSN-assisted cyclic signal amplification was developed for sensitive and selective detection of miRNAs. The method showed a high sensitivity with a low limit of detection and a wide linear range, demonstrating great potential for clinical molecular diagnostics and biological research.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Zi-Tao Zhong, Ghazala Ashraf, Wei Chen, Lai-Bo Song, Shu-Jie Zhang, Bo Liu, Yuan-Di Zhao
Summary: Duplex-specific nuclease (DSN) is a promising tool for bioanalysis due to its unique capability to cleave DNA within duplexes. This study proposes a new sensing strategy by coupling DSN with DNA aptamer sequence for diverse analytes detection with signal amplification and cycling. The method shows satisfactory performance in detecting adenosine triphosphate, thrombin, and alpha-fetoprotein.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Yuanyuan Yao, Hongding Zhang, Tongtong Tian, Yixin Liu, Rendan Zhu, Ji Ji, Baohong Liu
Summary: A new detection method for miRNA-21 was proposed in this study, achieving ultra-sensitive and quantitative analysis through DSN signal amplification strategy and SERS technology. The method showed excellent performance in detecting miRNA-21 and was successfully applied for monitoring the expression levels of miRNA-21 in different cancer cell lines and human serum.
Article
Chemistry, Analytical
Xin Zhou, Hongmei Cao, Yong Zeng
Summary: In this study, a pneumatically automated microfluidic reactor device was designed to integrate analyte extraction/enrichment and DSNSA-mediated miRNA detection in a streamlined analysis workflow, achieving rapid and robust on-chip detection of miR-21 with reduced consumption of DSN enzyme. The optimized workflow demonstrated a limit-of-detection of 35 amol and has potential applications in clinical diagnosis and beyond.
Article
Chemistry, Analytical
Ghazala Ashraf, Zi-Tao Zhong, Muhammad Asif, Ayesha Aziz, Laibo Song, Shujie Zhang, Bo Liu, Wei Chen, Yuan-Di Zhao
Summary: In this study, a novel approach has been proposed to extend the sensing application of duplex specific nuclease (DSN) by coupling RNA aptamer. The method achieves target detection through fluorescent signal enhancement by cleaving hybridized probes with DSN. The assay has a low detection limit and can be applied for real-time detection of thrombin release from live cells.
Article
Chemistry, Analytical
Meiqing Liu, Haoran Li, Yanwei Jia, Pui-In Mak, Rui P. Martins
Summary: A method based on duplex-specific nuclease for direct detection of SARS-CoV-2 from virus RNA was developed, showing a detection sensitivity of 500 pM virus RNA. This simple and rapid RNA detection method is expected to complement the detection of viruses mutated at the PCR primer-binding regions.
Article
Biophysics
Yuecan Zhao, Junzhu Xiang, Hao Cheng, Xiaojuan Liu, Feng Li
Summary: The development of a novel flexible photoelectrochemical biosensor for miRNA determination showed promising potential for ultrasensitive disease diagnosis. The biosensor exhibited excellent analytical performance, selectivity, stability, flexibility, and practical applicability, making it a valuable tool for bioassays and early disease detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Xiao-Long Zhang, Sha-Sha Li, Yi-Jia Liu, Wei-Wei Liu, Ling-Qi Kong, Ya-Qin Chai, Xi-Liang Luo, Ruo Yuan
Summary: An ingenious three-dimensional DNA walker with high walking efficiency is developed by limiting the reaction space, providing a rapid and sensitive detection method for miRNA. Compared to traditional DNA walkers, this immobilized DNA walker shows improved kinetic and thermodynamic favorability, resulting in faster reaction speed and higher walking efficiency. By utilizing this walker, a sensitive sensing platform for rapid miRNA-21 detection is constructed with a limit of detection of 19.8 aM, allowing for highly sensitive biomarker detection in cancer cell lysates.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Xiao Zhao, Rong Liao, Yueming Tan, Wenfang Deng, Qingji Xie
Summary: In this study, a highly sensitive self-powered biosensing platform for miRNA detection was developed. The platform integrates a glucose/O2 biofuel cell, CRISPR-Cas12a cleavage, and DSN-assisted target recycling, enabling ultrasensitive sensing of miRNA-155.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Khouloud Djebbi, Biao Shi, Ting Weng, Mohamed Bahri, Mohamed Amin Elaguech, Jin Liu, Chaker Tlili, Deqiang Wang
Summary: Researchers have developed a highly sensitive and selective biosensor for detecting miRNA. By studying miRNA-21 as a model, they investigated the factors affecting DSN-assisted target recycling and successfully detected miR-21 as low as 170 aM under optimized conditions. This approach exhibits high selectivity and improved miRNA detection sensitivity through the synergistic effect between DSN enzyme activity and neutral DNA spacer.
Article
Chemistry, Analytical
Yunyun Ning, Cuiling Zhang, Chunyan Wang, Chuqing Zhou, Nana Gong, Qiang Wang, Ye Zhu
Summary: In this study, a highly sensitive and specific electrochemical strategy was proposed for accurate detection of miRNA-21. The strategy utilized DSN-assisted target recycling and DNA self-assembled FeNxC nanocatalytic network, and achieved ultrasensitive detection of miRNA-21.
ANALYTICA CHIMICA ACTA
(2022)
Article
Spectroscopy
Qingyou Cai, Fanfan Wang, Jingying Ge, Zhiguo Xu, Mei Li, Hui Xu, Hua Wang
Summary: In this study, a G-wire-based self-quenched fluorescence probe and its application in ultrasensitive microRNA detection were reported. The approach combined target-activated isothermal cascade amplification for signal amplification. The results showed that the approach achieved detection of miRNA within a wide linear range and demonstrated selectivity and sensitivity in human spiked serum samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Yuetong Yang, Derong Kong, Yungen Wu, Yiheng Chen, Changhao Dai, Chang Chen, Junhong Zhao, Shi Luo, Wentao Liu, Yunqi Liu, Dacheng Wei
Summary: Graphene field-effect transistors (GFETs) offer simple and label-free diagnostics for sensitive and specific detection of microRNAs (miRNAs). Researchers have developed a GFET biosensor based on tetrahedral DNA nanostructures (TDN) that facilitates catalytic hairpin assembly (CHA) reaction for ultrasensitive detection of miRNA-21. The biosensor exhibits excellent analytical performance and has been successfully applied in complex serum environments and cell lysates, showing promising potential in bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuetong Yang, Derong Kong, Yungen Wu, Yiheng Chen, Changhao Dai, Chang Chen, Junhong Zhao, Shi Luo, Wentao Liu, Yunqi Liu, Dacheng Wei
Summary: A graphene field-effect transistor (GFET) biosensor based on a tetrahedral DNA nanostructure (TDN)-assisted catalytic hairpin assembly (CHA) reaction (TCHA) has been developed, allowing sensitive and specific detection of miRNA-21. The TDN structures in the biosensing interface facilitate the CHA reaction and prevent unwanted entanglements, aggregation, and adsorption of probes on the graphene channel. The biosensor exhibits ultra-sensitive detection of miRNA-21, with a detection limit of 5.67 x 10(-19) M, three orders of magnitude lower than that achieved by graphene transistors with single-stranded DNA probe functionalization of the channel. Furthermore, the biosensor demonstrates excellent performance in terms of selectivity, stability, and reproducibility, showing promising potential in bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ensheng Xu, Yahui Feng, Haitang Yang, Peng Li, Lu Kong, Wei Wei, Songqin Liu
Summary: The study developed an ultrasensitive multiple miRNAs detection strategy based on SDA and ACHA reactions, showing a linear relationship in the range of 0.1 fM to 10 fM for miRNA-122 and miRNA21 with low detection limits. The electrochemical sensor can be applied to detect other miRNAs by changing template strands, and the electrode can be regenerated by incubating with specific DNA strands.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Junji Li, Zi-Xuan Liu, Yi-Xuan Li, Guofang Shu, Xue-Ji Zhang, Robert S. Marks, Dan Shan
Summary: In this study, a tailored metal-organic framework transducer material Cu BDC(NH2)@2-MI was synthesized using 2-methylimidazole as a competitive ligand, which allowed for the modulation of its morphology and catalytic activity. The 2-MI-doped MOF exhibited well-tuned morphology and showed efficient performance in electrochemical H2O2 reduction. This novel design could potentially enable the utilization of proprietary MOFs materials beyond the laboratory.
Article
Chemistry, Multidisciplinary
Yongchao Song, Dongdong Wang, Zehua Li, Lirong Wang, Chuan Fan, Xuecheng He, Tailin Xu, Xueji Zhang
Summary: By integrating a jigsaw-like multifunctional mini-pillar platform, this study enables multi-mode sensing in individual microdroplets, including electrochemical, fluorescence, SERS, and colorimetric detection. The platform reduces false positive analysis and shows potential for in situ disease diagnosis and point-of-care testing.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Yuchun Qiao, Jinya Du, Rujiao Ge, Huiting Lu, Chaoxiong Wu, Jinze Li, Shuangshuang Yang, Shah Zada, Haifeng Dong, Xueji Zhang
Summary: This study developed microneedles made of gelatin methacryloyl and graphene oxide for the enrichment and sensitive detection of multiple microRNA biomarkers from skin interstitial fluid. The microneedles exhibited robust mechanical properties and fast sampling kinetics, enabling effective miRNA analysis and personalized diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Jeyaraj Jeyavani, Ashokkumar Sibiya, Narayanan Gopi, Shahid Mahboob, Khalid A. Al-Ghanim, Fahad Al-Misned, Zubair Ahmed, Mian Nadeem Riaz, Balasubramanian Palaniappan, Marimuthu Govindarajan, Baskaralingam Vaseeharan
Summary: Polypropylene microplastics are the main contaminant in aquatic environments, but research on their toxicity is limited. This study demonstrates that acute exposure to polypropylene microplastics causes immobility in Daphnia similis and enhances the generation of reactive oxygen species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Analytical
Yan Huang, Yiyi Zhang, Wenbin Hao, Huiting Lu, Haifeng Dong, Xueji Zhang
Summary: To achieve fast, convenient, and highly sensitive detection of microRNA (miRNA), palladium-gold (Pd-Au) bimetallic nanoplates with high photothermal effect were synthesized and combined with locked nucleic acid (LNA) detection probe to develop a photothermal lateral flow locked nucleic acid biosensor (P-LFLNAB) for miRNA detection. The P-LFLNAB enables sensitive and selective miRNA-21 detection with detection limit down to 0.094 pM using a smartphone with external thermal imager, which is 639.5 times lower than that of the conventional gold nanoparticles based colorimetric lateral flow biosensor and the most sensitive enzyme-free lateral flow biosensor for miRNA detection. Besides, the P-LFLNAB achieved single-base mismatch discrimination and can quantify signals in various cell lysates. This highly sensitive, selective and portable P-LFLNAB with smartphone readout has great potential in point-of-care test of miRNAs-related biomedical application.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Xiangdan Meng, Sirong Sun, Chenchen Gong, Junyan Yang, Zhou Yang, Xueji Zhang, Haifeng Dong
Summary: In this study, we report the design of a Ti-based metal-organic framework (MIL) and silver nanoparticles (Ag NPs) heterostructure, MIL@Ag. It is found that the introduction of Ag NPs significantly improves the electron transfer efficiency and O2 adsorption capacity of MIL, leading to enhanced reactive oxygen species (ROS) generation for sonodynamic therapy (SDT). Under ultrasound irradiation, the activated electrons and holes from MIL and Ag NPs produce superoxide radicals and hydroxyl radicals, respectively, improving the therapeutic efficiency of SDT. MIL@Ag-PEG-mediated SDT demonstrates effective cancer cell killing and bacterial infection eradication. Thus, MIL@Ag-PEG provides a promising strategy for enhancing SDT performance through rational heterostructure design of sonosensitizers.
Article
Chemistry, Analytical
Shuangshuang Yang, Jinya Du, Menglian Wei, Yan Huang, Yufan Zhang, Yeyu Wang, Jinze Li, Wei Wei, Yuchun Qiao, Haifeng Dong, Xueji Zhang
Summary: Rapid, simple, sensitive and reliable approaches for quantifying biogenic amines in food samples are essential for food safety. Here, the authors reported a Fe3O4@Au nanoparticles based multimodal readout LFIA for rapid quantification of putrescine and histamine. The assay showed high sensitivity and could be easily expanded to detect other biogenic amines.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Tingting Wu, Xushun Liu, Hanjun Chen, Ying Liu, Yu Cao
Summary: This study proposes an in situ exosomal miRNA sensing biochip based on multi-branched localized catalytic hairpin assembly (MLCHA) and photonic crystals (PCs). The MLCHA probes can penetrate exosomes nondestructively and generate amplified fluorescence signals upon recognition of the target miRNA. The fluorescence signal is further enhanced by PCs to improve sensitivity. The developed biosensor not only detects exosomal miRNA in a concentration-dependent manner but also distinguishes samples from cancer and healthy states, showing potential for non-invasive clinical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Mengyun Zhou, Yong Luo, Lirong Wang, Chuan Fan, Tailin Xu, Xueji Zhang
Summary: The outbreak of COVID-19 has posed a great challenge to global health systems. A rapid and sensitive POCT diagnostic platform for SARS-CoV-2 that can be deployed on-site is crucial in containing the epidemic. A portable microdroplet detection platform with temperature control and micro-stirring has been developed, enabling high-throughput and ultrafast COVID-19 diagnosis. The platform, utilizing a PN junction as the temperature controller and incorporating an ultrasonic micro-stirring unit, shows good linearity and low LOD, providing results in a short time. This integrated platform offers a promising tool for point-of-care diagnosis of COVID-19 in resource-limited settings.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Environmental
Kun Yu, Mengjie Li, Huining Chai, Qian Liu, Xin Hai, Mingwei Tian, Lijun Qu, Tailin Xu, Guangyao Zhang, Xueji Zhang
Summary: This study presents a portable colorimetric and electrochemical dual-mode sensor based on MOF-818 nanozyme for the detection of cellular secreted H2O2 and H2S. The sensor enables accurate detection of H2O2 and H2S through colorimetric and electrochemical methods. By combining with a smartphone and miniature electrochemical analyzer, it allows for in situ detection of H2O2 and H2S released from live cells, showcasing potential applications in various fields.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Tingyi Yan, Guangyao Zhang, Kun Yu, Huining Chai, Mingwei Tian, Lijun Qu, Haifeng Dong, Xueji Zhang
Summary: Maintaining normal levels of vitamin C is crucial for a healthy immune system. The development of real-time non-invasive wearable sensors for monitoring vitamin C in sweat shows promise in personalized health management. In this study, a smartphone light-driven enzyme-free wearable photoelectrochemical sensor was presented for precise monitoring of sweat vitamin C.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Multidisciplinary Sciences
Arsh E. Noor, Zaib Un Nisa, Salma Sultana, Khalid A. Al-Ghanim, F. Al-Misned, Mian Nadeem Riaz, Z. Ahmed, Shahid Mahboob
Summary: Heavy metal contamination is a serious global environmental issue that harms soil and plant growth. Wastewater has become an essential source of irrigation in developing countries and rural areas due to the scarcity of canal water. This study found that spinach irrigated with sanitary wastewater exceeded the permissible limits of heavy metal concentration, posing potential health risks to humans.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2023)
Article
Nanoscience & Nanotechnology
Ying Liu, Xin Wang, Hanjun Chen, Tingting Wu, Yu Cao, Zhihong Liu
Summary: Chemodynamic therapy (CDT) is a promising strategy for cancer treatment, but its efficiency is limited by insufficient intracellular H2O2 level. This study presents a method to enhance CDT by silencing the CAT gene to accumulate H2O2 in tumor cells. Cu-siRNA nanocomposites are fabricated and modified with HA for active tumor targeting. Cu+ is generated by reduction of released Cu2+ by GSH, which then catalyzes H2O2 to produce toxic hydroxyl radicals to kill tumor cells. CAT siRNA efficiently inhibits H2O2 consumption, leading to H2O2 accumulation. Cu-siRNA@ HA exhibits good biocompatibility and therapeutic efficiency, offering a new way to supply H2O2 in CDT with potential for clinical application.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Yaodong Hu, Shuaibing Yu, Nan Ma, Jinming Kong, Xueji Zhang
Summary: In this study, a Rose Bengal-Mediated photoinduced atom transfer radical polymerization (photo-ATRP) method was used to achieve highly sensitive detection of target DNA (tDNA), which is of great significance in the field of early disease screening.
Article
Chemistry, Multidisciplinary
Lian-Hua Xu, Wen-Ju Wang, Xue-Ji Zhang, Serge Cosnier, Robert S. Marks, Dan Shan
Summary: The complexity of synthesizing cobalt phosphides (CoP) has led researchers to focus on simplifying the preparation process. In this study, amino tris(methylene phosphonic acid) (ATMP, L1) and melamine (MA, L2) were assembled into two-dimensional organic nanostructures through supramolecular assembly. This simplified preparation process resulted in a catalyst (N-CoP/NC) with enhanced oxygen reduction reaction (ORR) and hydrogen evolution reaction (HER) performance. Density Functional Theory (DFT) calculations provided insight into the catalytic pathway of N-CoP/NC.
