期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 41, 页码 27594-27610出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b10417
关键词
biomimetic; apatite; peptide; nanofiber; bone extracellular matrix
资金
- National Natural Science Foundation of China [81371697, 81271183, 81570979]
- Peking University's 985 Grants
- Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences [ODBS-2014-001]
- Chongqing Research Program of Basic Research and Frontier Technology [cstc2016jcy-jA0199]
- Program for Innovation Team Building at Institutions of Higher Education in Chongqing
- Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education
- Outstanding Doctoral Scientific Research Fund of Chongqing Medical University
The design and development of functional biomimetic systems for programmed stem cell response is a field of topical interest. To mimic bone extracellular matrix, we present an innovative strategy for constructing drug-loaded composite nanofibrous scaffolds in this study, which could integrate multiple cues from calcium phosphate mineral, bioactive molecule, and highly ordered fiber topography for the control of stem cell fate. Briefly, inspired by mussel adhesion mechanism, a polydopamine (pDA)-templated nanohydroxyapatite (tHA) was synthesized and then surface-functionalized with bone morphogenetic protein-7-derived peptides via catechol chemistry. Afterward, the resulting peptide-loaded tHA (tHA/pep) particles were blended with polycaprolactone (PCL) solution to fabricate electrospun hybrid nanofibers with random and aligned orientation. Our research demonstrated that the bioactivity of grafted peptides was retained in composite nanofibers. Compared to controls, PCL-tHA/pep composite nanofibers showed improved cytocompatibility. Moreover, the incorporated tHA/pep particles in nanofibers could further facilitate osteogenic differentiation potential of human mesenchymal stem cells (hMSCs). More importantly, the aligned PCL-tHA/pep composite nanofibers showed more osteogenic activity than did randomly oriented counterparts, even under nonosteoinductive conditions, indicating excellent performance of biomimetic design in cell fate decision. After in vivo implantation, the PCL-tHA/pep composite nanofibers with highly ordered structure could significantly promote the regeneration of lamellar-like bones in a rat calvarial critical-sized defect. Accordingly, the presented strategy in our work could be applied for a wide range of potential applications in not only bone regeneration application but also pharmaceutical science.
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