Review
Biotechnology & Applied Microbiology
Zhimin Yang, Ping Yi, Zhongyue Liu, Wenchao Zhang, Lin Mei, Chengyao Feng, Chao Tu, Zhihong Li
Summary: Tremendous advances in tissue engineering and regenerative medicine have shown the potential of 3D bioprinting using hydrogels to solve bone and joint defects. The encapsulation of stem cells in hydrogels can improve their uncontrolled proliferation, migration, and differentiation. This study focuses on the characterization and application of stem cell-laden hydrogel-based 3D bioprinting for bone and cartilage tissue engineering, highlighting the relationship between biophysical properties and the regeneration of bone and cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Kalindu Perera, Ryan Ivone, Evelina Natekin, Cheryl. A. Wilga, Jie Shen, Jyothi U. Menon
Summary: Cartilage defects cause joint pain, swelling, and stiffness, affecting patients' quality of life. Current treatment options have limitations, leading to an increased interest in cartilage tissue engineering. 3D bioprinting is a promising method to generate functional structures for cartilage repair, providing flexibility and support needed for implantation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Polymer Science
Sivaraj Mehnath, Velluchamy Muthuraj, Murugaraj Jeyaraj
Summary: The synthesis of a snail shell-derived bioceramic/polymeric 3D scaffold was investigated in this study. The scaffold showed improved mechanical strength, compressive strength, and porosity due to the incorporation of hydroxyapatite (HAP). The HAP grafting on the surface of the scaffold promoted mineralization and facilitated the attachment and proliferation of cells. Additionally, the scaffold exhibited biocompatible properties and demonstrated antibacterial activity against gram-positive/gram-negative bacteria, making it suitable for bone tissue engineering applications.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Engineering, Biomedical
Gagan K. Jalandhra, Thomas G. Molley, Tzong-tyng Hung, Iman Roohani, Kristopher A. Kilian
Summary: This study developed a unique printing platform that integrates soft and hard materials concurrently through freeform printing of mineralized constructs within tunable micro-gel suspensions containing living cells. By tuning the microgel stiffness and filler content, chondrogenesis and osteogenesis can be differentially directed within the same construct, enabling the fabrication of osteochondral interfaces in a single step. This versatile one-pot biofabrication approach has the potential to aid in bone disease modeling and tissue engineering.
ACTA BIOMATERIALIA
(2023)
Article
Materials Science, Multidisciplinary
Irene Chiesa, Carmelo De Maria, Giovanni Vozzi, Riccardo Gottardi
Summary: Each year, thousands of patients face life-threatening ear, nose, and throat disorders, such as tracheal stenosis, or conditions that affect their psychosocial well-being, such as microtia. Tissue engineering offers an exciting alternative by creating bioartificial constructs using three-dimensional scaffolds and human cells that can grow and develop. Bioprinting technologies, which utilize additive manufacturing, are particularly useful in addressing the complex geometries of ear, nose, and throat, allowing for the creation of patient-specific scaffolds with high design flexibility and repeatability.
Article
Engineering, Biomedical
Xin Sun, Zhenjiang Ma, Xue Zhao, Wenjie Jin, Chenyu Zhang, Jie Ma, Lei Qiang, Wenhao Wang, Qian Deng, Han Yang, Jinzhong Zhao, Qianqian Liang, Xiaojun Zhou, Tao Li, Jinwu Wang
Summary: 3D bioprinted scaffolds loaded with MSNs/BMP-4 can effectively improve bone repair in diabetic patients by enhancing cell viability, promoting osteogenesis, and regulating macrophage polarization to improve the inflammatory microenvironment.
BIOACTIVE MATERIALS
(2021)
Review
Pharmacology & Pharmacy
Markel Lafuente-Merchan, Sandra Ruiz-Alonso, Fatima Garcia-Villen, Idoia Gallego, Patricia Galvez-Martin, Laura Saenz-del-Burgo, Jose Luis Pedraz
Summary: This article reviews the advantages of using 3D bioprinting techniques for osteochondral regeneration, discusses the commonly used biomaterials, cell types, and active molecules, and considers the most recent promising results.
Review
Chemistry, Applied
Sumit Murab, Aastha Gupta, Malgorzata Katarzyna Wlodarczyk-Biegun, Anuj Kumar, Patrick van Rijn, Patrick Whitlock, Sung Soo Han, Garima Agrawal
Summary: This review highlights the importance of alginate-based hydrogel inks for fabricating 3D printed scaffolds for bone and cartilage regeneration. The fundamentals of direct extrusion 3D bioprinting method are discussed, and a comprehensive overview of various alginate-based hydrogel ink formulations that have been used so far is provided. The requirements of hydrogel inks and 3D printed scaffolds to achieve similarity to the native tissue environment are summarized.
CARBOHYDRATE POLYMERS
(2022)
Article
Materials Science, Multidisciplinary
Alperen Abaci, Gulden Camci-Unal, Murat Guvendiren
Summary: Three-dimensional (3D) bioprinting is an emerging technology that can fabricate functional tissues and organs, replicating native tissue function. With its precise positioning of cellular materials and utilization of medical images, 3D bioprinting has enormous potential in biomedical applications, such as tissue engineering and regenerative medicine. It is a rapidly progressing field that has shown clinically relevant uses.
Article
Biochemistry & Molecular Biology
Kevin Behan, Alexandre Dufour, Orquidea Garcia, Daniel Kelly
Summary: Articular cartilage has limited healing potential, leading to the development of degenerative diseases such as osteoarthritis. Current clinical strategies aim to regenerate articular cartilage, but recreating a fully functional, load-bearing tissue remains a significant challenge due to a lack of biomaterials that mimic native tissue and provide appropriate regeneration cues.
Review
Cell & Tissue Engineering
Carlos M. Chiesa-Estomba, Ana Aiastui, Iago Gonzalez-Fernandez, Raquel Hernaez-Moya, Claudia Rodino, Alba Delgado, Juan P. Garces, Jacobo Paredes-Puente, Javier Aldazabal, Xabier Altuna, Ander Izeta
Summary: This study conducted a systematic review on the role of 3D bioprinting scaffolding for nasal cartilage defects repair, with a focus on cellular sources, types of scaffold materials, biochemical evaluation, histological analysis, in-vitro and in-vivo studies, and animal models used. The results showed high heterogeneity in mechanical evaluation, common patterns of collagen expression in scaffolds, and potential limitations in the translational investigation due to heterotopic scaffold implantation and lack of quantitative histological data. Further research is needed to address these issues in the growing field of 3D bioprinting for nasal cartilage defects repair.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Yiting Ze, Yanxi Li, Linyang Huang, Yixin Shi, Peiran Li, Ping Gong, Jie Lin, Yang Yao
Summary: Mature vasculature is crucial for the survival of bioengineered tissue constructs. However, creating fully vascularized tissue constructs remains a great challenge in tissue engineering. Indirect 3D bioprinting has gained attention for its ability to create complex vascular network-like channels in thick tissue constructs while maintaining endothelial cell activity. Biodegradable materials play an important role in tissue engineering, especially in the selection of scaffolds and sacrificial materials in indirect 3D bioprinting.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Alan Avila-Ramirez, Kevin Catzim-Rios, Carlos Enrique Guerrero-Beltran, Erick Ramirez-Cedillo, Wendy Ortega-Lara
Summary: This study presents the preparation of a novel ink for bone and cartilage tissue restoration, focusing on the synthesis of bioceramic materials, integration into a biopolymeric base matrix, and characterization of functional composite materials for future manufacturing using techniques such as 3D printing.
Review
Engineering, Biomedical
Mehran Khajehmohammadi, Negar Bakhtiary, Niyousha Davari, Soulmaz Sarkari, Hamidreza Tolabi, Dejian Li, Behafarid Ghalandari, Baoqing Yu, Farnaz Ghorbani
Summary: This review focuses on the factors that should be considered during the fabrication of cell-laden protein-based hydrogels (PBHs) for bioprinting, including biophysical and biochemical factors. It also discusses the key considerations for bioprinting PBHs and their impact on tissue regeneration.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Cell & Tissue Engineering
Robert Choe, Eoin Devoy, Erfan Jabari, Jonathan D. Packer, John P. Fisher
Summary: Osteoarthritis is a common musculoskeletal disorder that causes joint pain and deformity, significantly affecting patients' quality of life. The osteochondral unit is composed of three layers, each contributing to unique biomechanical properties. Understanding the role of the osteochondral interface is important for tissue engineering advancements.
TISSUE ENGINEERING PART B-REVIEWS
(2021)