Article
Biotechnology & Applied Microbiology
Jinggui Nie, Danting Chen, Jing Ye, Yanbin Lu, Zhiyuan Dai
Summary: In this study, a preparative separation protocol for three terpenoids from Sargassum fusiforme was established using high-speed countercurrent chromatography combined with preparative high-performance liquid chromatography. The isolated compounds showed purities above 95% and their chemical structures were identified by H-1 and C-13 nuclear magnetic resonance spectroscopy.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Chemistry, Analytical
Yujie Li, Qiangqiang Jia, Min Zhang, Lu Kang, Zihan Li, Yu Liu, Hongyang Zhang, Ping Hu
Summary: In this study, three glucaric acids were successfully isolated and purified from Leonurus japonicus Houtt. using a combination of high-speed countercurrent chromatography and semi-preparative high-performance liquid chromatography. Cation exchange resin chromatography was applied to remove alkaloids and enrich the glucaric acid fractions. The obtained compounds were identified as 2-syringyl glucaric acid, 2,4-disyringyl glucaric acid, and 3,4-disyringyl glucaric acid, with the latter being reported for the first time.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Yueqi Wang, Liping Guo, Chunming Liu, Sainan Li
Summary: The study systematically examined the active components in T. repens, evaluated their activity, separated and purified them, and identified potentially effective inhibitors. The method used was simple, fast, and efficient, providing a theoretical basis and technical platform for the development of natural medicines.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Xiaohua Jiang, Yueyuan Chen, Xuerong Yang, Xiaojie Yan, Fenglai Lu, Zhangbin Liu, Dianpeng Li
Summary: In this study, an efficient strategy using high-speed countercurrent chromatography combined with preparative high-performance liquid chromatography was developed to separate diterpenoid compounds from complex crude extracts of Salvia bowleyana Dunn roots. The results demonstrated that this method can effectively isolate diterpenoids, including minor components, from complex natural products.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Multidisciplinary Sciences
Theresa Anna Lehmair, Peter Poschlod, Christoph Reisch
Summary: This study aimed to compare genetic and epigenetic variation patterns in different habitats in Central European grasslands and identify their drivers. Through the analysis of genetic and epigenetic variation of Trifolium pratense L., it was found that there were low levels of genetic and epigenetic differentiation among populations and between habitat types, and the two were not interdependent. Genetic variation was significantly isolated by habitat dissimilarity, while epigenetic variation was affected by environment.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Chemistry, Analytical
Yi Tao, Lin Chen, Faxiang Pu, Zhangfu Xie, Shengqiang Tong, Jizhong Yan
Summary: High-speed countercurrent chromatography was utilized for the first time to preparatively separate the anti-tumor compound antroquinonol from solid fermentation culture of Antrodia camphorata, achieving a high purity of 97.12% through optimized extraction conditions and solvent system.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Analytical
Liu-Qing Sheng, Jia-Lei Ma
Summary: Two antimalaria alkaloids, febrifugine and isofebrifugine, were successfully separated from total alkaloids of Dichroa febrifuga roots by one-step preparative countercurrent chromatography with a selected biphasic solvent system. The separated alkaloids had high purity levels, providing valuable experimental data for drug research.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Plant Sciences
Zhenfei Yan, Lijun Sang, Yue Ma, Yong He, Juan Sun, Lichao Ma, Shuo Li, Fuhong Miao, Zixin Zhang, Jianwei Huang, Zengyu Wang, Guofeng Yang
Summary: In this study, a high-quality de novo genome assembly for red clover was obtained, with improved assembly quality compared to previously published genomes. The annotated gene models can serve as important resources for molecular genetic breeding and legume evolution studies. Additionally, the evolutionary relationships between red clover and closely related species were analyzed, providing a foundation for studying the evolution of clover and legumes, genomic analysis of forage grass, and improvement of agronomic traits.
Article
Chemistry, Medicinal
Danting Chen, Yating Jin, Di Hu, Jing Ye, Yanbin Lu, Zhiyuan Dai
Summary: A method for batch preparation of fucoxanthin from brown algae was established using ultrasonic-assisted extraction and elution-extrusion countercurrent chromatography, resulting in high yield and high purity. The optimal conditions for preparation were determined, and fucoxanthin was successfully obtained from Sargassum fusiforme, Laminaria japonica, and Undaria pinnatifida. The study demonstrated the applicability of the method to brown algae, improving the utilization value of raw materials.
Article
Biochemical Research Methods
Honglei Bao, Wenyu Sun, Yang Jin, Chaoyue Wang, Xiang Wang, Hengmian Sun, Chu Chu, Shengqiang Tong
Summary: Maslinic acid and corosolic acid were successfully separated from Eriobotrya japonica leaves with high purity using a two-step countercurrent chromatographic separation technique. Two biphasic solvent systems were selected based on the partition performance of the main structural isomeric pentacyclic triterpenes. The recoveries of the two isomers were over 90%, demonstrating the effectiveness of the separation method.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemistry & Molecular Biology
Weidong Dai, Maria Ramos-Jerz, Dongchao Xie, Jiakun Peng, Peter Winterhalter, Gerold Jerz, Zhi Lin
Summary: In this study, two epimeric EPSF compounds, R-EGCG-cThea and S-EGCG-cThea, were successfully isolated from white tea using CPC. Two different biphasic solvent systems were used for pre-fractionation experiments, resulting in high purity recovery of the compounds. Further bioactivity evaluation showed inhibition effects on alpha-glucosidase with specific IC50 values for R- and S-EGCG-cThea.
Article
Chemistry, Analytical
Yuan-Hang Chang, Lian Yu, Han Yang, Jian Xu, Yu-Jie Fu
Summary: This research investigated an integrated method for the extraction and separation of naphthoquinones and diarylheptanes from green walnut husks. The results showed that this method achieved high efficiency in a short time, providing a time and solvent-saving approach for the extraction of natural products from plants.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemical Research Methods
Yan Zhou, Hu Shan, Haitao Lu
Summary: An efficient method using high-speed countercurrent chromatography (HSCCC) was developed for the separation and purification of three flavonoids from Oroxylum indicum. The optimal extraction conditions were determined through single-factor and orthogonal experiments, leading to the successful one-step separation of oroxin A, oroxin B, and baicalein with high purities and recoveries. The chemical structures of the compounds were confirmed, and their potential as scavengers of free radicals was demonstrated.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Biochemical Research Methods
Shunjie Zhang, Haijun Chen, Xiaodie Deng, Hailu Chen, Chanjuan Guo, Li Wan, Aihua Peng, Lijuan Chen
Summary: Counter-current chromatography (CCC) is a widely used liquid-liquid separation technique, and high aspect ratio rectangular horizontal (RH) tubing has shown advantages in improving resolution and throughput. This study verifies the high stationary phase retention and throughput of RH tubing on a semi-preparative CCC apparatus, and successfully manufactures a lighter and larger volume semi preparative bobbin with thin-wall RH tubing.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Multidisciplinary
Ju -Zhao Liu, Le-Le Wen, Xiao-Li Tian, Yu-Jie Fu, Qi Cui
Summary: In this study, a green and efficient ultrasonic-assisted extraction method was optimized for the extraction of polyphenols from Hibiscus manihot L. flowers. A two-step high-speed counter-current chromatography method was established to isolate and prepare eight polyphenols with purities exceeding 95%. The proposed methods provide a cost-effective and powerful strategy for the extraction and preparation of high-purity polyphenols from H. manihot L. flowers.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Biochemical Research Methods
Yao Qin, Lina Chen, Xiaojing Yang, Ying Tang, Senlin Li, Chunming Liu
Article
Biochemical Research Methods
Sainan Li, Senlin Li, Chengyu Liu, Chunming Liu, Yuchi Zhang
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2017)
Article
Chemistry, Analytical
Sainan Li, Jianxu Zhang, Senlin Li, Chunming Liu, Shu Liu, Zhiqiang Liu
JOURNAL OF SEPARATION SCIENCE
(2017)
Article
Chemistry, Analytical
Senlin Li, Sainan Li, Yu Huang, Chunming Liu, Lina Chen, Yuchi Zhang
JOURNAL OF SEPARATION SCIENCE
(2017)
Article
Chemistry, Medicinal
Ying Tang, Senlin Li, Sainan Li, Xiaojing Yang, Yao Qin, Chunming Liu, Yuchi Zhang
MEDICINAL CHEMISTRY RESEARCH
(2017)
Article
Food Science & Technology
Chunming Liu, Sainan Li, Rong Tsao, Senlin Li, Yuchi Zhang
JOURNAL OF FUNCTIONAL FOODS
(2017)
Article
Electrochemistry
Senlin Li, Xin Zhang, Hui Chen, Haiman Hu, Jingdong Liu, Yurong Zhang, Yongxin Pan, Yuanhui Zheng
ELECTROCHIMICA ACTA
(2020)
Article
Chemistry, Physical
Senlin Li, Wenjing Zhang, Jingdong Liu, Yurong Zhang, Yuanhui Zheng
Summary: By designing a novel silica@gallium core-shell structure for the cathode of lithium-sulfur batteries, highly efficient electrocatalysis of lithium polysulfides was achieved, leading to a high reversible capacity and excellent cycling stability. The proposed mechanism involves a highly conductive electron-given surface provided by the liquid metal gallium shell on the silica surface in thick electrodes for reversible electrocatalysis of lithium polysulfides in lithium-sulfur batteries.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Physical
Xin Zhang, Haixin Chen, Hui Chen, Senlin Li, Yurong Zhang, Yuanhui Zheng
Summary: The performance of NTP anode material for ALIBs was improved by carbon coating and Fe-F co-doping, with further enhancements achieved by using stainless steel as a gasket. The NTP-Fe-0.1-F-0.1/C material showed outstanding electrochemical properties and long cycling lifetime, attributed to enhanced structural stability and electron/Li+ ion transfer kinetics.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Engineering, Multidisciplinary
Zhang WenJing, Li SenLin, Zhang YuRong, Wang XingHui, Liu JingDong, Zheng YuanHui
Summary: This research successfully prepared a quasi-solid-state electrolyte based on ethoxylated trimethylolpropane triacrylate through a one-step in-situ photopolymerization method. The electrolyte has a three-dimensional network structure, overcoming the contradiction between the plasticizer-related conductivity and the thickness-dependent mechanical performance of traditional solid-state electrolytes. The electrolyte demonstrates excellent ionic conductivity, high lithium ion transference number, and a wide electrochemical window. When applied in lithium-ion batteries, it significantly reduces interface resistance and enhances electrode/electrolyte interface stability, leading to improved initial discharge capacity and Coulombic efficiency.
SCIENCE CHINA-TECHNOLOGICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Senlin Li, Jian Shang, Meilin Li, Minwei Xu, Fanbin Zeng, Hang Yin, Yongbing Tang, Cuiping Han, Hui-Ming Cheng
Summary: Electroactive organic materials with tailored functional groups are crucial for aqueous Zn-organic batteries due to their green and renewable features. In this study, a new N-heteroaromatic material (HATN-PNZ) is designed and synthesized, and it demonstrates ultrahigh performance as a cathode for Zn-ion batteries. By optimizing the molecular structure, the material exhibits enhanced electrical conductivity, high structural stability, and impressive capacity, rate capability, and cycle life.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Physical
Hui Chen, Xipin Zhang, Senlin Li, Yuanhui Zheng
Summary: In this study, a free-standing sulfur cathode with efficient polysulfide adsorption and electrochemical catalysis is reported. A cationic polymer binder is used to produce a viscoelastic microstructure, facilitate lithium-ion transportation, and regulate polysulfides electrostatically. The resulting Li-S battery exhibits a high initial areal capacity of 7.09 mAh cm-2 (equivalent to 344.5 Wh kg-1 and 471.6 Wh l-1) and stable areal capacity of 4.48 mAh cm-2 (equivalent to 217.6 Wh kg-1 and 235.8 Wh l-1) during cycling at a low electrolyte/sulfur ratio of 4 mu L mg-1.
ACS ENERGY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Fanbin Zeng, Senlin Li, Sanlue Hu, Minling Qiu, Guobin Zhang, Meilin Li, Caiyun Chang, Hongliang Wang, Minwei Xu, Lirong Zheng, Yongbing Tang, Cuiping Han, Hui-Ming Cheng
Summary: Calcium ion batteries (CIBs) are potential energy storage devices due to their abundant resources and low cost. However, slow kinetics limit their performance. In this study, a solvation regulation strategy based on donor number (DN) was proposed to enhance the storage of Ca2+ in sodium vanadate (NVO) cathode. The use of solvent with low DN facilitated the migration of Ca2+ and promoted the insertion of Ca2+ into the NVO electrode, resulting in high capacity and high-rate performance.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Physical
Haiman Hu, Hui Chen, Wensong Wang, Senlin Li, Yurong Zhang, Jingdong Liu, Yuanhui Zheng
Summary: A nickel-catalyzed carbonization method was used to synthesize three-dimensional nickel@N-doped carbon-nanotube foams for lithium-sulfur batteries. The resulting cathode exhibited high sulfur loading, high initial capacity, and good cyclic stability, while also effectively suppressing the polysulfide shuttle effect and providing good conductivity.
JOURNAL OF ALLOYS AND COMPOUNDS
(2022)
