期刊
JOURNAL OF PHYSIOLOGICAL SCIENCES
卷 69, 期 2, 页码 399-408出版社
SPRINGER JAPAN KK
DOI: 10.1007/s12576-018-00658-1
关键词
Monocarboxylates; Monocarboxylate transporter; SMCT; Lactate; PDZ; PDZK1
类别
资金
- JSPS (KAKENHI) [15590233, 18590900, 21390073, 26461258, 18K08200]
- Salt Science Research Foundation [0524, 0721]
- Nakatomi Foundation
- Gout Research Foundation of Japan
- Kyorin University School of Medicine
- Grants-in-Aid for Scientific Research [21390073, 18K08200, 15590233, 26461258, 18590900] Funding Source: KAKEN
Sodium-coupled monocarboxylate transporters SMCT1 (SLC5A8) and SMCT2 (SLC5A12) mediate the high- and low-affinity transport of lactate in the kidney, but their regulatory mechanism is still unknown. Since these two transporters have the PDZ-motif at their C-terminus, the function of SMCTs may be modulated by a protein-protein interaction. To investigate the binding partner(s) of SMCTs in the kidney, we performed yeast two-hybrid (Y2H) screenings of a human kidney cDNA library with the C-terminus of SMCT1 (SMCT1-CT) and SMCT2 (SMCT2-CT) as bait. PDZK1 was identified as a partner for SMCTs. PDZK1 coexpression in SMCT1-expressing HEK293 cells enhanced their nicotinate transport activity. PDZK1, SMCT1, and URAT1 in vitro assembled into a single tri-molecular complex and their colocalization was confirmed in the renal proximal tubule in vivo by immunohistochemistry. These results indicate that the SMCT1-PDZK1 interaction thus plays an important role in both lactate handling as well as urate reabsorption in the human kidney.
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