期刊
JOURNAL OF LEUKOCYTE BIOLOGY
卷 105, 期 1, 页码 177-186出版社
WILEY
DOI: 10.1002/JLB.3A0718-251R
关键词
carbon tetrachloride; CXCL2; mannan-binding lectin; neutrophil; sterile liver injury
资金
- National Natural Science Foundation of China [81671568, 81571608, 81771771]
- Science and Technology Planning Project of Guangdong Province [2016A020215106]
- Natural Science Foundation of Guangdong Province [2017A030313542, 2017A030313597]
Noninfectious liver injury, including the effects of drugs and diet, is a major cause of liver diseases worldwide. The innate inflammatory response to hepatocyte death plays a crucial role in the outcome of liver injury. Mannan-binding lectin (MBL) is a pattern recognition molecule of the innate immune system, which is primarily produced by liver. MBL deficiency occurs with high frequency in the population and is reported associated with predisposition to infectious diseases. We here observed that genetic MBL ablation strongly sensitizes mice to sterile liver injury induced by carbon tetrachloride (CCl4). Aggravated liver damage was shown in CCl4-administrated MBL-/- mice, as evidenced by severe hepatocyte death, elevated serum alanine aminotransferase and lactate dehydrogenase activity, and enhanced production of inflammatory cytokines. Mechanistic studies established that MBL deficiency caused increased chemokine CXCL2 production from liver macrophages upon CCl4 stimulation, thereby promoting the hepatic recruitment of neutrophils and subsequent liver damage. Furthermore, MBL-mediated protection from CCl4-induced liver injury was validated by administration of an MBL-expressing liver-specific adeno-associated virus, which effectively ameliorated the hepatic damage in CCl4-treated MBL-/- mice. We propose that MBL may be exploited as a new therapeutic approach in the treatment of chemical-induced sterile liver injury in patients with MBL deficiency.
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