期刊
JOURNAL OF INFECTIOUS DISEASES
卷 219, 期 8, 页码 1294-1306出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiy615
关键词
Klebsiella pneumoniae; Sap transporter; liver abscess; intestinal cell; virulence
资金
- Ministry of Science and Technology
- National Taiwan University
- National Taiwan University Hospital
- E-Da Hospital
- E-Da Hospital-National Taiwan University Hospital Joint Research Program
Klebsiella pneumoniae is an important human pathogen causing hospital-acquired and community-acquired infections. Systemic K. pneumoniae infections may be preceded by gastrointestinal colonization, but the basis of this bacterium's interaction with the intestinal epithelium remains unclear. Here, we report that the K. pneumoniae Sap (sensitivity to antimicrobial peptides) transporter contributes to bacterial-host cell interactions and in vivo virulence. Gene deletion showed that sapA is required for the adherence of a K. pneumoniae blood isolate to intestinal epithelial, lung epithelial, urinary bladder epithelial, and liver cells. The sapA mutant was deficient for translocation across intestinal epithelial monolayers, macrophage interactions, and induction of proinflammatory cytokines. In a mouse gastrointestinal infection model, sapA yielded significantly decreased bacterial loads in liver, spleen and intestine, reduced liver abscess generation, and decreased mortality. These findings offer new insights into the pathogenic interaction of K. pneumoniae with the host gastrointestinal tract to cause systemic infection. Klebsiella pneumoniae Sap transporter-mediated cell adherence, intestinal epithelial translocation, macrophage interactions, in vivo colonization, liver abscess formation, and mouse mortality. Findings of the current study elucidate the pathogenic interactions of K. pneumoniae with gastrointestinal tract to cause systemic infection.
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