期刊
JOURNAL OF INFECTION AND CHEMOTHERAPY
卷 25, 期 4, 页码 314-317出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jiac.2018.09.005
关键词
CTX-M type ESBL-producing; Enterobacteriaceae; Japanese community; Asymptomatic healthy individuals; Molecular epidemiology
资金
- e-ASIA Joint Research program (e-ASIA JR)
- AMED, Japan [16jm0210048h0001]
- JSPS KAKENHI, Japan [17J08848, 17H04663]
- Grants-in-Aid for Scientific Research [17J08848, 17H04663] Funding Source: KAKEN
This study was performed to characterize CTX-M type extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae carriage in asymptomatic health individuals, which has not been well investigated, in a community of the Okinawa prefecture, Japan. Fecal samples were voluntary collected from asymptomatic healthy individuals who were going to take a routine medical checkup. The collected fecal samples were inoculated on MacConkey agar supplemented with 2 mu g/ml of cefotaxime and incubated at 37 degrees C. Randomly selected three lactose-fermented colonies per each sample were analyzed. Genetic relatedness among the CTX-M type ESBL-producing Enterobacteriaceae isolates were performed by pulsed-field gel electrophoresis (PFGE) after confirmation of ESBL phenotype and determination of bacterial species. Location of bla(CTX-M) was confirmed by S1-PFGE, I-CeuI-PFGE and the Southern blotting hybridization. ESBL-producing Enterobacteriaceae was isolated from 32 (12.2%) of the collected 263 fecal samples, and 96 ESBL-producing Enterobacteriaceae isolates were obtained. CTX-M type ESBL-producing Escherichia coli B2 were major (67 isolates, 72.0%) and 40 (59.7%) of the 67 CTX-M type ESBL-producing E. coli B2 were E. coli B2-ST131. Three CTX-M type ESBL-producing E. coli B2-ST131 isolates from asymptomatic healthy individuals showed similar PFGE band patterns as five CTX-M type ESBL-producing E. coli B2-ST131 isolates from a hospital locates in the same area of the target community. Chromosomally-transferred bla(CTX-M) was observed in 10.0% of the examined CTX-M type ESBL-producing Enterobacteriaceae isolates. We report current situation CTX-M type ESBL-producing Enterobacteriaceae carriage in asymptomatic healthy individuals of the Okinawa prefecture, Japan. In addition, our results indicated that worldwide distributed CTX-M type ESBL-producing E. coli B2-ST131 has been spread in a community. Therefore monitoring of ESBL-producing Enterobacteriaceae in healthy individuals is important. (C) 2018 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
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