Article
Biochemistry & Molecular Biology
Jinxing Song, Mengxiang Wang, Yongkun Du, Bo Wan, Angke Zhang, Yuhang Zhang, Guoqing Zhuang, Pengchao Ji, Yanan Wu, Gaiping Zhang
Summary: African swine fever virus (ASFV) is a serious threat to pigs and boars, causing significant production and economic losses. A dominant ASFV-specific linear B cell epitope was identified using immune informatics techniques, which is important for understanding host immunity and developing diagnostic guidelines and vaccine designs. The confirmation of dominant epitopes through serological analysis is feasible.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Mei Huang, Hanghui Zheng, Weixiong Tan, Chengwei Xiang, Niran Fang, Wenting Xie, Lianghai Wen, Dingxiang Liu, Ruiai Chen
Summary: Immunization of pigs with SFV replicon-based vaccine candidates can promote specific T cell and humoral immunity against ASFV. Individual heterogeneity in response to the vaccine requires personalized analysis. Signaling pathways mediated by Toll-like receptors, C-type lectin receptors, IL17 receptors, NOD-like receptors, and nucleic acid sensors are correlated with antibody production and IFN-γ secretion in PBMCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Lulu Wang, Dan Fu, Weldu Tesfagaber, Fang Li, Weiye Chen, Yuanmao Zhu, Encheng Sun, Wan Wang, Xijun He, Yu Guo, Zhigao Bu, Dongming Zhao
Summary: African swine fever (ASF) is a highly contagious disease that poses a significant threat to the world pig industry. Researchers have made significant progress in developing vaccines against ASF, and a diagnostic method has been established to differentiate between wild-type ASFV-infected and vaccinated animals.
Article
Virology
Sabal Chaulagain, Gustavo A. Delhon, Sushil Khatiwada, Daniel L. Rock
Summary: The study revealed that ASFV CD2v activates the NF-kappa B signaling pathway, inducing IFN signaling and apoptosis in swine lymphocytes/macrophages, which may contribute to the lymphocyte apoptosis observed in lymphoid tissue during ASFV infection in pigs.
Article
Microbiology
Chun Miao, Sicheng Yang, Junjun Shao, Guangqing Zhou, Yunyun Ma, Shenghui Wen, Zhuo Hou, Decai Peng, HuiChen Guo, Wei Liu, Huiyun Chang
Summary: African swine fever virus (ASFV) is a highly lethal viral disease (ASF) in pigs that causes significant losses in China and other countries. Developing a vaccine and diagnosis technology for ASFV is crucial in controlling its spread. This study generated 17 monoclonal antibodies (mAbs) targeting the p72 protein of ASFV and identified seven linear B cell epitopes. Although these mAbs did not show neutralizing activity, they provided new insights into the antigenic regions of ASFV p72 and had potential diagnostic value.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Anusyah Rathakrishnan, Ana L. Reis, Vlad Petrovan, Lynnette C. Goatley, Katy Moffat, Yuan Lui, Mai T. Vuong, Shinji Ikemizu, Simon J. Davis, Linda K. Dixon
Summary: A study constructed an attenuated genotype of African swine fever virus by introducing amino acid substitutions in the CD2v protein to reduce its binding ability to red blood cells and virus persistence in blood. The vaccinated pigs showed moderate replication of the virus and 100% protection against the virulent African swine fever virus. The results support further evaluation of this vaccine candidate.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Microbiology
Siyuan Liu, Peiyang Ding, Yongkun Du, Dongna Ren, Yilan Chen, Minghui Li, Xueke Sun, Siqiao Wang, Zejie Chang, Ruiqi Li, Gaiping Zhang
Summary: In this study, the extracellular region of ASFV CD2v protein was successfully expressed in the 293F expression system, and four monoclonal antibodies targeting this protein were prepared. These monoclonal antibodies showed good reactivity to ASFV and recognized an important linear epitope. The identified epitope was highly conserved in the Chinese epidemic strain of ASFV and Georgia2008/1 strain, indicating a potential protective effect.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mohamed Imdhiyas, Suvam Sen, Nagendra Barman, Lukumoni Buragohain, Yashpal Malik, Sachin Kumar
Summary: This study used computational methods to identify B- and T-cell epitopes for the p30 and p54 proteins of the African swine fever virus (ASFV). By analyzing amino acid sequences, predicting structures, and performing molecular dynamics simulations, multiple linear and discontinuous B-cell epitopes were identified, as well as T-cell epitopes related to SLA-1, 2, and 3. These findings are important for understanding the immune characteristics of ASFV proteins and designing diagnostics and vaccines.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Immunology
Qi Gao, Yunlong Yang, Weipeng Quan, Jiachen Zheng, Yizhuo Luo, Heng Wang, Xiongnan Chen, Zhao Huang, Xiaojun Chen, Runda Xu, Guihong Zhang, Lang Gong
Summary: African swine fever virus poses serious threats to the swine industry, with the mortality rate at 100% and no effective vaccine available. CD2v and MGF360-505R genes are involved in modulating the immune response. Deficiency in CD2v and MGF360-505R in ASFV leads to decreased apoptosis in cells by inhibiting the NF-kappa B signaling pathway and IL-1 beta mRNA transcription, suggesting a potential novel strategy for ASFV replication in the host.
Article
Immunology
Wenming Liu, Hengchun Li, Bo Liu, Tianxing Lv, Chenchen Yang, Si Chen, Liqiang Feng, Liangxue Lai, Ziyuan Duan, Xinwen Chen, Pingchao Li, Suhua Guan, Ling Chen
Summary: African swine fever (ASF) is a highly contagious disease that poses a serious threat to the global pig industry. This study explored the safety and immunogenicity of an adenovirus-vectored multi-antigen cocktail vaccine against ASF. The vaccine, administered through simultaneous intramuscular and intranasal administration, effectively stimulated immune responses and provided protection against ASF in mice and swine. Further evaluation of this vaccination strategy is needed for safe and effective control of ASF.
EMERGING MICROBES & INFECTIONS
(2023)
Review
Virology
Xiaojie Zheng, Shengming Nie, Wen-Hai Feng
Summary: African swine fever is a highly contagious and deadly viral disease that has spread globally, causing economic losses. The lack of an effective vaccine makes understanding the virus's immune evasion mechanisms crucial for vaccine development.
Article
Veterinary Sciences
Suphachai Nuanualsuwan, Tapanut Songkasupa, Prakit Boonpornprasert, Nutthakarn Suwankitwat, Walaiporn Lohlamoh, Chackrit Nuengjamnong
Summary: The African swine fever virus can be indirectly transmitted through contaminated objects, feed, pork, and pig products. Heat treatment of swill can effectively eliminate the virus, but there are inconsistent recommendations regarding the temperature and time of heat treatment. This study assessed the thermal inactivation of ASFV in different swill formulae and developed a model to predict inactivation at other temperatures.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Review
Microbiology
Alexander Schaefer, Giulia Franzoni, Christopher L. Netherton, Luise Hartmann, Sandra Blome, Ulrike Blohm
Summary: African swine fever virus (ASFV) poses a threat to global pig populations, causing a deadly hemorrhagic disease. While myeloid cells are the primary target for ASFV, T cell responses are also impacted by the infection. The current understanding of the complex responses and the development of a commercially available vaccine are still limited.
Article
Microbiology
Rui Jia, Gaiping Zhang, Yilin Bai, Hongliang Liu, Yumei Chen, Peiyang Ding, Jingming Zhou, Hua Feng, Mingyang Li, Yuanyuan Tian, Aiping Wang
Summary: In this study, monoclonal antibodies against the ASFV CD2V protein were developed, and B cell epitopes were identified. This provides efficient epitopes for the development of ASFV subunit vaccines.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Michele D. Zajac, Neha Sangewar, Shehnaz Lokhandwala, Jocelyne Bray, Huldah Sang, Jayden McCall, Richard P. Bishop, Suryakant D. Waghela, Rakshith Kumar, Tae Kim, Waithaka Mwangi
Summary: The pp220 polyprotein of ASFV contains multiple epitopes that induce robust immune responses in pigs and are conserved among different ASFV genotypes, making it a promising candidate for a subunit vaccine.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Olga Zakharova, Fedor Korennoy, Ivan Iashin, Nadezhda N. Toropova, Andrey E. Gogin, Denis Kolbasov, Galina Surkova, Svetlana M. Malkhazova, Andrei A. Blokhin
Summary: Leptospirosis, a zoonotic infectious disease caused by pathogenic bacteria, shows seasonality and is influenced by socio-economic factors like human population density and agricultural production. Climatic factors have a weaker influence on the disease incidence. Future climate projections suggest a potential increase in leptospirosis cases in the Russian Arctic.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Galina Koltsova, Andrey Koltsov, Sergey Krutko, Natalia Kholod, Edan R. Tulman, Denis Kolbasov
Summary: Research shows that the CD2v (EP402R) protein plays a crucial role in inducing protective immunity against virulent African swine fever virus challenges in homologous live-attenuated vaccines. Experimental vaccination/challenge studies in pigs have revealed that the virus with a specific deletion of the EP402R gene fails to provide protection against deadly viral challenges.
Article
Veterinary Sciences
Olga Zakharova, Ilya A. Titov, Andrey E. Gogin, Timofey A. Sevskikh, Fedor Korennoy, Denis Kolbasov, Levon Abrahamyan, Andrey A. Blokhin
Summary: African swine fever (ASF) is a viral contagious disease that affects domestic pigs and wild boars, causing significant economic damage globally. The intensive spread of ASF in the Russian Far East region since 2019 raises concerns for wildlife population and food resources. The study aims to determine the genotype of ASF virus, analyze the spatio-temporal patterns of outbreaks, and assess the potential reduction in regional fauna due to expected depopulation of wild boars.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Yaroslava Chalenko, Olga Kolbasova, Elena Pivova, Mariam Abdulkadieva, Olga Povolyaeva, Egor Kalinin, Denis Kolbasov, Svetlana Ermolaeva
Summary: This study investigated the interaction between L. monocytogenes and sheep cells, and found that the invasion efficiency was influenced by the InlB factor. Different InlB isoforms may contribute to the hypervirulence of certain clonal groups towards ruminants, and L. monocytogenes can effectively multiply once it enters sheep cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alexey D. Sereda, Anna S. Kazakova, Viktor V. Dmitrenko, Denis Kolbasov
Summary: This report evaluates the immunobiological properties of a candidate live vaccine against African swine fever (ASF) using two tests. The results demonstrate that attenuated virus strains can modulate the cellular link of protective immunity without negatively affecting the humoral immune response.
Article
Infectious Diseases
Pavel A. Andriyanov, Pavel A. Zhurilov, Daria D. Kashina, Anastasia Tutrina, Elena A. Liskova, Irina Razheva, Denis Kolbasov, Svetlana A. Ermolaeva
Summary: This study reports the first isolation of E. anophelis strain in Russia and its isolation from raw cow's milk. The strain displayed resistance to multiple antimicrobials and carried various antibiotic resistance determinants and virulence factors.
Article
Multidisciplinary Sciences
Alexey D. Sereda, Anna S. Kazakova, Sanzhi G. Namsrayn, Mikhail E. Vlasov, Denis V. Kolbasov
Summary: African swine fever is an infectious disease without a registered effective vaccine. Experimental vaccines, particularly live attenuated ones, have been intensively developed in recent years. However, the variability in experimental approaches and the lack of correlation between virus genotyping and cross-protective efficacy make it challenging to interpret and compare trial results.
Article
Virology
Alexey D. Sereda, Mikhail E. Vlasov, Galina S. Koltsova, Sergey Y. Morgunov, Dmitry A. Kudrjashov, Irina P. Sindryakova, Olga L. Kolbasova, Valentina M. Lyska, Andrei Y. Koltsov, Sergey P. Zhivoderov, Elena Y. Pivova, Vladimir M. Baluishev, Andrey E. Gogin, Denis V. Kolbasov
Summary: The African swine fever virus (ASFV) is causing a pandemic that is threatening the global pig industry. A laboratory-attenuated ASFV strain, Katanga-350, shows promise as a potential basis for developing a recombinant vaccine against ASF viruses belonging to seroimmunotype I.
Article
Virology
Natalia Kholod, Andrey Koltsov, Sergey Krutko, Edan R. Tulman, Sanzhi Namsrayn, Gerald E. F. Kutish, Sergey Belov, Alexey Korotin, Mikhail Sukher, Galina Koltsova
Summary: African swine fever (ASF) is a contagious disease caused by ASF virus (ASFV) with a lack of vaccines. Attenuated viruses were obtained from cultured cell lines, which provided protection against infection. A study on Congo-a (KK262) virus showed differences in replication and virulence. Genome sequencing of the attenuated KK262 strain revealed a deletion and genetic changes compared to the virulent homologue K49. These findings contribute to understanding ASFV attenuation and identification of potential virulence genes for vaccine development.
Article
Agriculture, Dairy & Animal Science
Andrey Koltsov, Mikhail Sukher, Natalia Kholod, Sanzhi Namsrayn, Sodnom Tsybanov, Galina Koltsova
Summary: Pork is an important source of protein in the human diet and swine diseases cause economic losses to the global pig industry. This article reports the successful isolation of the swinepox virus in Russia, which showed low pathogenicity in pigs. The study also demonstrates the use of nasal and oral swabs for PCR diagnosis and virus isolation of swinepox.
Article
Agriculture, Dairy & Animal Science
Andrey Koltsov, Sergey Krutko, Natalia Kholod, Mikhail Sukher, Sergey Belov, Alexey Korotin, Galina Koltsova
Summary: African swine fever (ASF) causes serious economic losses to the global pig industry. The development of effective and safe vaccines for ASF is actively ongoing. Experimental vaccines based on live attenuated strains of the ASF virus (ASFV) obtained by deleting viral genes responsible for virulence have shown the most effectiveness. However, the deletion of the same gene in different ASFV isolates can lead to different outcomes.
Article
Infectious Diseases
Olga I. Zakharova, Andrey A. Blokhin, Ivan V. Yashin, Olga A. Burova, Denis V. Kolbasov, Fedor I. Korennoy
Summary: This study conducts a retrospective analysis of African swine fever (ASF) outbreaks in the Nizhny Novgorod region of Russia, focusing on the factors contributing to the spread of ASF in domestic pig and wild boar populations. The study identifies significant environmental and sociodemographic factors associated with ASF outbreaks and highlights the importance of monitoring and control measures in the pig products supply chain. The findings can be applied not only in the Nizhny Novgorod region but also in other regions with similar conditions.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Agriculture, Dairy & Animal Science
Olga Kolbasova, Timofey Sevskikh, Ilya Titov, Denis Kolbasov
Summary: This study investigated the virological and genetic aspects of caprine arthritis encephalitis (CAE) virus in the Republic of Mordovia, Russian Federation. The presence of the viral genome and antibodies against it were detected in goat blood samples, confirming the positive result of PCR through nucleotide sequencing. The obtained sequence was found to be identical to small ruminant lentiviruses in the GenBank database. Phylogenetic analysis revealed a cluster with an isolate from the Tver region in 2008. This research provides important insights into the distribution and classification of SLRVs in different regions of the Russian Federation.
Review
Veterinary Sciences
Sezim Monoldorova, Galina Koltsova, Ilya Titov, Inkyu Yoo, Andrey Gogin, Jonguk Jeong, Hyeon-Seop Byeon, Seungri Yun, Yunhee Chang, Shin-Seok Kang, Denis Kolbasov, Bo-Young Jeon
Summary: African swine fever (ASF) is a highly lethal viral disease in swine for which an effective vaccine is urgently needed. Subunit vaccines targeting protective antigens of ASFV and DNA vaccines inducing cellular immunity have shown partial protection in pigs.
THAI JOURNAL OF VETERINARY MEDICINE
(2022)
