期刊
JOURNAL OF CLINICAL INVESTIGATION
卷 129, 期 1, 页码 93-105出版社
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI121341
关键词
-
资金
- National Institute of Allergy and Infectious Disease, National Institutes of Health [U19AI082724, P01AI097092, U19AI109946, U19AI057266, HHSN272201400005C, P30CA014599, T32GM007281, T32AI007090]
- Complex Systems Scholar Award from the James S. McDonnell Foundation
- Centers of Excellence for Influenza Research and Surveillance grant [HHSN272201400005C]
Vaccines are among the most effective public health tools for combating certain infectious diseases such as influenza. The role of the humoral immune system in vaccine-induced protection is widely appreciated; however, our understanding of how antibody specificities relate to B cell function remains limited due to the complexity of polyclonal antibody responses. To address this, we developed the Spec-seq framework, which allows for simultaneous monoclonal antibody (mAb) characterization and transcriptional profiling from the same single cell. Here, we present the first application of the Specseq framework, which we applied to human plasmablasts after influenza vaccination in order to characterize transcriptional differences governed by B cell receptor (BCR) isotype and vaccine reactivity. Our analysis did not find evidence of long-term transcriptional specialization between plasmablasts of different isotypes. However, we did find enhanced transcriptional similarity between clonally related B cells, as well as distinct transcriptional signatures ascribed by BCR vaccine recognition. These data suggest IgG and IgA vaccine-positive plasmablasts are largely similar, whereas IgA vaccine-negative cells appear to be transcriptionally distinct from conventional, terminally differentiated, antigen-induced peripheral blood plasmablasts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据