Article
Genetics & Heredity
Biao Hu, Tianju Liu, Zhe Wu, Sem H. Phan, Karen Blyth
Summary: P53 can suppress the transcription and translation of C/EBP beta gene, and regulate the translation of LAP and LIP isoforms of C/EBP beta through the expression of eIF4E and eIF4E-BP1; furthermore, the 5'UTR region plays an important role in the differential control of LAP and LIP translation of C/EBP beta.
Article
Biochemistry & Molecular Biology
JanWillem Duitman, Leonie Hartl, Joris J. T. H. Roelofs, Maarten F. Bijlsma, C. Arnold Spek
Summary: C/EBP delta is downregulated in pancreatic ductal adenocarcinoma (PDAC) cells and associated with decreased patient survival and pro-tumorigenic properties. Systemic C/EBP delta facilitates pancreatic cancer metastasis, potentially through C/EBP delta-PAFR-dependent tumor cell extravasation.
Article
Biochemistry & Molecular Biology
HaiXu Song, Jiahao Liu, Xiaoxiang Tian, Dan Liu, Jiayin Li, Xiaojie Zhao, Zhu Mei, Chenghui Yan, Yaling Han
Summary: The transcription factor CCAAT/enhancer-binding protein ? (C/EBP?) plays a crucial role in the differentiation and cell cycle control of megakaryocytes (MKs), potentially aiding in the treatment of thrombocytopenia and other platelet-related disorders.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Cell Biology
Lixiang Sun, Xiaowei Zhang, Shuai Wu, Youxi Liu, Christian F. Guerrero-Juarez, Wenjie Liu, Jinwen Huang, Qian Yao, Meimei Yin, Jiacheng Li, Raul Ramos, Yanhang Liao, Rundong Wu, Tian Xia, Xinyuan Zhang, Yichun Yang, Fengwu Li, Shujun Heng, Wenlu Zhang, Minggang Yang, Chi-Meng Tzeng, Chao Ji, Maksim V. Plikus, Richard L. Gallo, Ling-juan Zhang
Summary: By conducting single-cell RNA sequencing on mouse skin, researchers have classified dermal fibroblasts into different non-adipogenic and adipogenic cell states. They have identified IL-1-NF-kB and WNT-b-catenin signaling pathways as regulators of adipogenesis. Neutrophils and WNT activation play roles in wound-induced adipogenesis and lipolysis, respectively, while sustaining WNT activation inhibits adipogenesis and contributes to myofibroblast formation.
Article
Biochemistry & Molecular Biology
Angelica Giuliani, Jacopo Sabbatinelli, Stefano Amatori, Laura Graciotti, Andrea Silvestrini, Giulia Matacchione, Deborah Ramini, Emanuela Mensa, Francesco Prattichizzo, Lucia Babini, Domenico Mattiucci, Elena Marinelli Busilacchi, Maria Giulia Bacalini, Emma Espinosa, Fabrizia Lattanzio, Antonio Domenico Procopio, Fabiola Olivieri, Antonella Poloni, Mirco Fanelli, Maria Rita Rippo
Summary: This study found that the expression of MeCP2 protein is downregulated during adipogenic differentiation of hBMSCs. This downregulation is not dependent on MeCP2 DNA methylation or mRNA levels, but on miRNA regulation. miR-422a and miR-483-5p are upregulated during adipogenic differentiation of hBMSCs. Modulation of miR-422a and miR-483-5p levels can affect MeCP2 expression and adipogenic differentiation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Oncology
Li-Mei Zhang, Mo Li, Chen-Chen Tian, Tong-Tong Wang, Shu-Fang Mi
Summary: This study revealed that downregulation of CEBPA in ovarian cancer predicted poor prognosis. Knockout of CEBPA promoted various features of cancer in ovarian cancer cells, while overexpression of CEBPA suppressed these features.
Article
Microbiology
Wan-Shan Yang, Wayne W. Yeh, Mel Campbell, Lung Chang, Pei-Ching Chang
Summary: KDM4A is a histone lysine demethylase known as an oncogene in various cancers, mediating important epigenetic regulation in tumorigenesis. Through the use of KSHV as a model, it was discovered that KIKAT/LINC01061 triggers the shifting of KDM4A, potentially acting as an epigenetic mechanism in gene transactivation.
Article
Genetics & Heredity
Yibo Wang, Kai Hang, Li Ying, Jiaqi Wu, Xiaoyong Wu, Weijun Zhang, Lijun Li, Zhongxiang Wang, Jinwu Bai, Xiang Gao, Deting Xue, Zhijun Pan
Summary: Chaperone-mediated autophagy (CMA) protein LAMP2A plays a crucial role in regulating the adipo-osteogenesis process of MSCs. Co-expression analyses revealed the relationships between LAMP2A and other relevant genes. In vitro and in vivo experiments demonstrated that LAMP2A positively regulates mMSC osteogenesis, suppresses adipo-osteogenesis, and promotes fracture healing in a mouse model.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Review
Cell Biology
Twan J. J. de Winter, Roeland Nusse
Summary: Wnt/β-catenin signaling pathway plays a role in suppressing differentiation of MSCs into adipocytes, promoting cell proliferation, and later promoting osteogenesis. Studies have shown that during the differentiation of MSCs into preadipocytes, Wnt signaling is regulated by endogenous Wnt inhibitors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Neena Lala-Tabbert, Hamood AlSudais, Francois Marchildon, Dechen Fu, Nadine Wiper-Bergeron
Summary: The study shows that the transcription factor C/EBP beta is necessary to maintain quiescence of satellite cells in uninjured muscle. Loss of C/EBP beta in satellite cells promotes premature exit from quiescence, while forced expression of C/EBP beta inhibits proliferation by upregulating quiescence-associated genes. Additionally, caveolin-1 is identified as a direct transcriptional target of C/EBP beta required for cell cycle exit in muscle satellite cells expressing C/EBP beta.
Article
Physiology
Pengyuan Chen, Wanzi Hong, Ziying Chen, Flora Gordillo-Martinez, Siying Wang, Hualin Fan, Yuanhui Liu, Yining Dai, Bo Wang, Lei Jiang, Hongjiao Yu, PengCheng He
Summary: The transcription factor C/EBP alpha plays a crucial role in the osteochondrogenic transdifferentiation of vascular smooth muscle cells (VSMCs) and vascular calcification. C/EBP alpha regulates the expression of the osteochondrogenic gene SOX9, and its overexpression or knockdown affects VSMC calcification. Silencing SOX9 expression significantly inhibits phosphate- and calcium-induced VSMC calcification.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Immunology
Liyan Dong, Jiaxing Zhu, Anyi Deng, Junping Wei, Jiawei Li, Xinru Mao, Zhenghu Jia
Summary: The study aims to elucidate the correlation between histone demethylase and gastric cancer. The results show that histone modification plays an important role in gastric cancer, including downstream gene expression regulation and epigenetic effects. Histone methyltransferase and histone demethylases are involved in the regulation of histone methylation status, which in turn affects various biological processes involved in the recognition of histone methylation modification. This is especially important in the regulation of chromatin function and the occurrence of gastric cancer and embryonic development.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Simranjeet Kaur, Nok Yin Tam, Michael A. McDonough, Christopher J. Schofield, Wei Shen Aik
Summary: ALKBH5 is an enzyme responsible for demethylating RNA and has emerged as a potential target for cancer treatment. This study provides insight into the substrate recognition and demethylation mechanism of ALKBH5, and reveals its preference for specific sequences.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Immunology
Rohit Chandwani, Terry C. Fang, Scott Dewell, Alexander Tarakhovsky
Summary: This study identifies the histone demethylase Kdm4d/JMJD2d as a positive regulator of type I interferon responses. It demonstrates that the demethylase affects both enhancer transcription and dynamic H3K9me2 modification at associated promoters, thereby modulating transcriptional responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Endocrinology & Metabolism
Siresha Bathina, Reina Armamento-Villareal
Summary: Fractures associated with Type 2 diabetes (T2DM) are a public health concern in an obese and aging population. Patients with T2DM have normal or better bone mineral density but are at a higher risk for fractures. The main issue in T2DM is a reduction in bone formation due to dysfunction in osteoblast differentiation and survival. Obesity and T2DM are also associated with increased adipogenesis, potentially at the expense of reduced osteogenesis and myogenesis. Understanding the complex mechanisms involved in bone pathophysiology in obesity and T2DM is important for developing pharmacological targets to improve bone health.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Baoli Wang, Hongting Jin, Bing Shu, Ranim R. Mira, Di Chen
SCIENTIFIC REPORTS
(2015)
Article
Cell & Tissue Engineering
Yan Xie, Xiaochen Wang, Xiaowen Wu, Lijie Tian, Jie Zhou, Xiaoxia Li, Baoli Wang
Article
Biochemistry & Molecular Biology
Meng Chen, Liying Shan, Ying Gan, Lijie Tian, Jie Zhou, Endong Zhu, Hairui Yuan, Xiaoxia Li, Baoli Wang
Summary: MTSS1 plays a role in osteoblast differentiation and bone homeostasis by regulating Src-Wnt/beta-catenin signaling.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell & Tissue Engineering
Xiaoli Shi, Yunzhu Cen, Liying Shan, Lijie Tian, Endong Zhu, Hairui Yuan, Xiaoxia Li, Ying Liu, Baoli Wang
Summary: The study revealed that NDRG1 negatively regulated cell growth and modulated the differentiation of marrow stromal progenitor cells towards adipocytes, impacting the osteoblast differentiation. NDRG1 interacted with LRP6 and inactivated the canonical Wnt/beta-catenin signaling cascade in stromal progenitor cells. In vivo transfection of Ndrg1 siRNA prevented inactivation of Wnt signaling and improved osteoblasts reduction and fat accumulation in ovariectomized mice.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Jie Zhou, Junying Yang, Yuan Dong, Yaru Shi, Endong Zhu, Hairui Yuan, Xiaoxia Li, Baoli Wang
Summary: Our study has for the first time established the direct role of OSMR in regulating osteogenic differentiation of marrow stromal progenitor cells through ERK-mediated autophagy signaling. OSMR thus contributes to bone homeostasis through dual regulation of osteoblasts and osteoclasts. It also suggests that OSMR may be a potential target for the treatment of metabolic disorders such as osteoporosis.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Haijian Sun, Shuang Wang, Zheng Yang, Lijie Tian, Xiaoxia Li, Jie Zhou, Baoli Wang
Summary: In this study, the researchers found that CHRDL1 acts as an antagonist of BMPs by binding to them and blocking their interaction with BMP receptors. They revealed that CHRDL1 suppresses osteoblast differentiation while promoting adipocyte differentiation. Mechanistic exploration showed that CHRDL1 stabilizes IGFBP3 and differentially modulates AKT/mTORC1 signaling, thereby regulating the differentiation of osteoblasts and adipocytes.
Article
Biochemistry & Molecular Biology
Shan Zhang, Yanru You, Yachong Li, Hairui Yuan, Jie Zhou, Lijie Tian, Ying Liu, Baoli Wang, Endong Zhu
Summary: Foxk1 promotes adipocyte differentiation by facilitating nuclear translocation and upregulating the transcriptional activity of the Ppar gamma 2 promoter.
Article
Cell & Tissue Engineering
Xiaowen Wu, Xiaochen Wang, Liying Shan, Jie Zhou, Xin Zhang, Endong Zhu, Hairui Yuan, Baoli Wang
Summary: The loss of NFIX gene affects endochondral ossification and mineralization in bone, with NFIX inducing osteoblast differentiation and impairing adipocyte formation by regulating HMGA1 expression. HMGA1 plays a role in osteoblast commitment and mediates NFIX function by activating canonical Wnt signaling pathways.
